|
Download
poster
449kb
pdf? |
|
Abstract for Automated Miniaturization of Promega’s Kinase-Glo®
Luminescent Kinase Assay in Low-Volume 384- and 1536-Well Formats
Brad Larson, B.A.1, Tracy Worzella, M.S.1, Michael Bjerke, M.S.1,
Aoife Gallagher, Ph.D.2 and Eric Matthews, M.S.3
1Promega Corporation, Madison, WI, 2Deerac Fluidics, Dublin, Ireland,
3BMG Labtech
Protein kinases play a vital role in cellular activity, ranging from cell
proliferation to cell death. Their substrates encompass a wide range of chemical
structures such as proteins, peptides, sugars, lipids, etc. They are also implicated in
many
diseases, including cancer, inflammatory responses, and Alzheimer’s
disease.
This makes them ideal targets for high-throughput screening of potential
drug
targets. Because of increased demands on screening facilities, the
ability to
miniaturize assays has become imperative. We have developed automated
Kinase-Glo® methods in low-volume 384- and 1536-well plate formats using
the Deerac Fluidics Equator™ NS 808 nanoliter-scale noncontact liquid handler.
Luminescent kinase activity was measured with the BMG LABTECH PHERAstar
reader. The Kinase-Glo® Assay is a homogeneous method for measuring kinase
activity from virtually any kinase/substrate combination by quantifying
ATP
remaining in solution following a kinase reaction. Excellent Z′-factor
scores and
accurate IC50 values in reaction volumes as low as 2μl, coupled with the
extended
luminescent half-life of this reagent chemistry, make this assay ideal
for all high-throughput
applications. |
