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Abstract for Automating Promega CellTiter-Glo^® Luminescent Cell Viability and Caspase-Glo^® 3/7 Assays in Low-Volume 384 and 1536-Well Formats

Tracy Worzella, M.S.¹, Brad Larson, B.A.¹, Michael Bjerke, M.S.¹, Aoife Gallagher, Ph.D.² and Eric Matthews, M.S.^3
1Promega Corporation, Madison, WI, ²Deerac Fluidics, Dublin, Ireland, ³BMG LABTECH, Offenburg, Germany

We have demonstrated the scalability of the CellTiter-Glo^® and Caspase-Glo^® 3/7 assays for high-throughput cell-based screening in low-volume 384- and 1536-well formats. The Deerac Fluidics Equator™ NS 808 Eight Tip Low Volume Dispenser was used for non-contact delivery of sample and reagent. The multifunctional, PMT-based BMG LABTECH PHERAstar reader was used for signal detection. Excellent Z’-factor, %CV, and linear range data was obtained. Together, these scalable assays and instrumentation offer ideal tools for HTS users studying drug effects on cells during the drug discovery process.

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