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Abstract for Apo-ONE® Homogeneous Caspase-3/7 Assay:
A Flexible Conduit Uniting the Drug Discovery and Development
Processes
Andrew L. Niles, Jean Humpal-Winter and Michael Bjerke, Promega
Corporation
Modulation of the apoptotic cell death process continues to be
the focus of intensive pharmaceutical research targeted at the
elimination of human infirmities as diverse as reperfusion ischemia,
neurodegeneration, and cancers. Exciting advances in automation and
screening technologies have drastically improved assay throughput,
but have rarely provided an integrated means to identify new
chemical entity actives as well as characterize their biological
attributes in a convenient format. Herein, we detail the
implementation of our Apo-ONE® Homogeneous Caspase-3/7 Assay in
apoptotic screen development with adherent and suspension cells, in
a mock HTS screen for actives, in a secondary screen for candidate
compound potency, and finally in an in vitro toxicology testing
application. These successful demonstrations with the Biomek® 2000
automated module, emphasize the assay’s broad flexibility and
sensitivity in the drug discovery and development process. |
