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Abstract for A Rapid and Sensitive "Mix-and-Measure" Luminescent
Cell Viability Assay
Richard A. Moravec, Michael T. Beck, Nancy Murphy, Mary Sobol,
Rita R. Hannah, Keith V. Wood and Terry L. Riss
Promega Corporation, 2800 Woods Hollow Rd, Madison, WI 53711
We have developed a simple and highly sensitive homogeneous assay
(CellTiter-Glo™ Assay) for quantifying viable cells in multi-well
plates. The assay is based on detection of adenosine triphosphate
(ATP), which is an indicator of viability present in all cells. The
homogeneous “add-mix-measure” protocol is performed by adding a
single reagent directly to cells cultured in serum-containing
medium, mixing, and recording luminescence with a luminometer or CCD
imaging device. The luminescence is proportional to ATP
concentration and correlates to the number of viable
cells over 3-4 logs depending on the type of multiwell plates and
cells used. Assay sensitivity is sufficient to detect 4 cells/well
in a 384 well format. The bioluminescent “glow” signal can be read
within 10 minutes of reagent addition and has a half-life of ~5
hours, allowing flexibility for batch or continuous automated
processing. We will demonstrate the usefulness of the CellTiter-Glo™
Assay for high-throughput cytotoxicity
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