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Abstract for MAO-Glo™ Assay: Fast and Easy Luminescent Assay to
Measure Monoamine Oxidase
M.P. Valley1, W. Zhou2, E.M. Hawkins1, J. Shultz1, T. Good2, D.
Good2, K.V. Wood1
1Promega Corporation, 2800 Woods Hollow Rd. Madison, WI 53711,
2Promega Biosciences Inc, 277 Granada Dr., San Luis Obispo, CA 93401
The MAO-Glo™ Assay, a luminescent method for sensitive
quantitation of monoamine oxidase (MAO) activity, enables rapid
assessment of potential inhibition caused by new chemical entities.
The homogeneous assay format is compatible with laboratory
automation, or can be performed manually (e.g., 96 samples in under
90 minutes). The assay is suitable for both MAO A and MAO
B, and data generated for known MAO inhibitors yielded Ki and IC50
values consistent with published literature. The signal-to-noise
ratio is >1000 and Z' values ~ 0.95. Data concerning compounds from
the LOPAC library known as MAO A inhibitors or substrates were
extracted from the screen. |
