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Abstract for P450-Glo™ Luminescent Cytochrome P450 Assays
James J. Cali, Mary Sobol, Dongping Ma, Susan Frackman,
Promega
Corporation,
2800 Woods Hollow Rd., Madison, WI 53711
P450-Glo™ Luminescent P450 Assays utilize derivatives of beetle
luciferin as P450 enzyme substrates. Luciferin is a substrate for
the light generating reaction of firefly luciferase. The derivatives
are inactive with luciferase but are metabolized by P450s to
luciferin, which in turn reacts with luciferase to make light. A
homogenous luciferase mixture is added directly to P450 reactions
that use luciferin derivatives as substrates. The amount of
luciferin produced by a P450 is directly proportional to light
output so light is used to measure P450 activity. Substrates are
provided for CYP1A1, 1A2, 1B1, 2C8, 2C9, 2C19, 2D6, 2J2, 3A4, 3A7,
4A11, 4F3B, 4F12 and 19. P450-Glo™ Assays detect P450 activities in
recombinant preparations, liver microsomes and cultured cells.
P450-Glo™ Assays provide a simple, homogenous and robust
luminescent assay format for rapid screening of multiple compounds. |
