The E. coli S30 T7 High-Yield Protein Expression System is designed to express up to 500µg/ml of protein in 1hr from plasmid vectors containing a T7 promoter and a ribosome binding site. The system provides an extract that contains T7 RNA polymerase for transcription and all other necessary components in the system are optimized for translation.
Protein is expressed in only one hour not days as with cell-free based expression.
No requirement to purchase additional reagents.
Express up to 500µg/ml of protein for multiple applications.
Convenient screening protocol for high-throughput needs.
Detect expressed proteins by coomassie staining or incorporation of a fluorescence or biotinylated modified tRNA.
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