Applications of a Smaller Brighter More Versatile Luciferase Reporter
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Kyle received his Ph.D. in Biochemistry from the University of Kansas Medical Center where he studied the interactions of prolactin with its receptor using techniques like cell-based assays, protein expression, and molecular biology. Prior to joining Promega, he did a post-doc at the University of Texas Southwestern Medical Center in the Molecular Department. Kyle studied the interaction of diphtheria toxin with its receptor, heparin-binding EGF-like growth factor, using techniques like site-directed mutagenesis, transfection and cell-based assays. Kyle first worked for five and a half years in the Promega Technical Services department helping researchers troubleshoot and refine their assays. Next, Kyle moved into product development working closely with R&D and recently joined the newly formed North American branch office focusing on cellular analysis and personal automation.
Kyle Hooper, Ph.D.
- See speaker bio
- Original Webinar Date: Tuesday, November 13, 2012
NanoLuc™ Luciferase, a 19kDa, monomeric luciferase gives a brighter signal and enables more sensitive measurement. The NanoLuc™/Nano-Glo™ Assay is ~100-fold brighter than either firefly or Renilla luciferase in vitro and in cells.
This seminar will address:
- Characteristics and uses of NanoLuc™ Luciferase
- Strategies for handling the bright luminescence
- Considerations for choosing Firefly or NanoLuc™ Luciferase
Applications of NanoLuc™ Luciferase as a fusion partner for:
- Protein translocation/degradation
- Protein/Protein interactions (utilizing BRET)
- Intracellular biosensors (utilizing BRET)