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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

RNA Extraction from Formalin Fixed Paraffin Embedded (FFPE) Tissue Enabling Next Generation Detection of Gene Fusions

MDx

Tuesday, January 24, 2017

Dr. William Rehrauer
University Wisconsin-Madison

Learn about the detection of novel RNA fusions via new next generation sequencing library prep methods, automated extraction of total nucleic acid and the utility of this research in the field of molecular oncology.

To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

Genomics

Tuesday, February 14, 2017

Doug Wieczorek, PhD

Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Successfully Overcoming the Challenges of Working with FFPE Samples

Genomics

Thursday, November 19, 2015

Trista Schagat, PhD

Formalin-fixed paraffin-embedded (FFPE) tissue samples are highly valuable, but difficult starting material for nucleic acid analysis. This webinar will discuss the different steps in molecular analysis using FFPE as a starting material and provide recommendations for optimizing each step from extraction through downstream applications.

Introduction to Real-Time PCR: Basic Principles and Chemistries

Genomics

Tuesday, November 10, 2015

Leta Steffen, PhD

Real-time PCR (qPCR) is the workhorse of most gene expression analysis. This webinar will provide an overview of basic qPCR principles compared to traditional PCR and common terms used in assay design and analysis.

Monitoring Protein:Protein Interactions in Living Cells Using a Small, Bright and Reversible Complementation System

Cell-Based Assays

Tuesday, October 27, 2015

Brock F. Binkowski, PhD.

This webinar will discuss NanoLuc® Binary Technology (NanoBiT™) for the study of intracellular protein interactions, which can be followed in real-time using a non-lytic assay protocol.

Antibody Internalization Assay Using a pH Sensor Fluorescent Dye

Proteomics

Tuesday, October 13, 2015

Nidhi Nath, PhD.

A novel approach is presented using a pH sensor fluorescent dye (pHAb dyes) is presented to identify antibodies suitable for receptor mediated internalization.

Circulating Cell-Free Nucleic Acids: Characteristics, Purification and Applications

MDx

Thursday, September 24, 2015

Douglas White

This webinar will describe a new automated circulating cell-free DNA purification method plus tips and tricks to improve success in various downstream applications. (Rescheduled from June to September.)

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