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Promega Webinars

Personalized Seminars for Your Lab

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. All presentations will be given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

Introduction to Nucleic Acid Purification: Purification Basics and Their Application to Different Sample Types

Genomics

Tuesday, February 09, 2016

Paraj Mandrekar

Nucleic acid purification is the first step in genomics research. Come learn about the basics of nucleic acid purification, approaches taken for purification of DNA or RNA and how purification chemistries are applied to common nucleic acid purification workflows.

Understanding Luminescent Reporter Assay Design

Cell-Based Assays

Tuesday, March 08, 2016

Carl Strayer, PhD

Luminescent reporter assays are powerful research tools for a variety of applications. This webinar explains critical aspects of reporter assay design to help you implement a robust assay that delivers meaningful results.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Complete FcγRIIIa Effector Function Analysis for mAb Biologics with High (V158) and Low (F158) Affinity Reporter Bioassays

Drug Discovery

Tuesday, February 24, 2015

Richard Somberg, Ph.D.

This webinar describes reporter-based ADCC assays that quantitatively measure Fc effector function for therapeutic antibodies using ADCC as MOA and how they can provide precise, accurate and reproducible data.

An Innovative System for Improving DNA Isolation from Diverse Plant Species

Genomics

Tuesday, February 10, 2015

Chris Moreland and Dr. Jackson Moeller

The extraction of genomic DNA from plants often involves tedious manual methods that produce suboptimal gDNA yields and purity. This webinar will introduce a new automated chemistry for the extraction of gDNA from plants that overcome the hassles of manual purification while dramatically increasing yields and purity.

Validation Studies on DNA Mixtures Using the PowerPlex® Fusion 6C System

Genetic Identity

Thursday, February 05, 2015

Dr. Antonio Alonso Alonso

This presentation will describe the validation studies conducted with the PowerPlex® Fusion 6C System using an Applied Biosystems® 3500 Genetic Analyzer for fragment detection, with special emphasis on the analysis of DNA mixtures. DNA mixture analysis included two DNA mixture series of female (major component)/male (minor component) and 15 body fluid mixed samples from previous GEDNAP (http://www.gednap.de) and GHEP-ISFG (http://www.gep-isfg.org) proficiency exercises.

It's All in the Details (or Small RNA): Simplified and Improved miRNA Purification from Tissue

MDx

Tuesday, January 27, 2015

Douglas Horejsh, Ph.D.

This webinar will discuss the growing field of miRNA research, the roles miRNA play in cells and a new solution for miRNA purification that simplifies purification without the use of any organic pre-processing.

Tracking Cellular Protein Localization and Movement in Cells with a Flexible Fluorescent Labeling Technology

Proteomics

Tuesday, January 13, 2015

Chad Zimprich, B.S.

This webinar will demonstrate how the HaloTag(R) Technology is uniquely suited to protein imaging analysis from simple cellular localization experiments to highly complex trafficking studies.

Factors to Consider When Choosing Cell-Based Assays for Use with 3D Cultures

Cell-Based Assays

Tuesday, December 16, 2014

Terry Riss, Ph.D.

This webinar is sponsored by Corning and presented by Promega describing the advantages and disadvantages of various 3D culture methods.

Efficiently Build Relevant In Vitro Models Using Human Stem Cell-Derived Tissue Cells, High Performance Transfection and Novel Multiplexed Reporter Techniques

Cell-Based Assays

Tuesday, December 09, 2014

Matthew Robers and Brian Jarecki, Ph.D.

This webinar will discuss how highly pure human tissue cells and new transfection techniques can be used to build relevant human cell models that recapitulate disease phenotypes and enable rapid, quantitative analysis of biological pathways.

Successfully Overcoming the Challenges of Working with FFPE Samples

Genomics

Tuesday, November 11, 2014

Trista Schagat, Ph.D.

Optimizing FFPE analysis, from extraction to downstream applications.

Massively Parallel Sequencing and STR Typing

Genetic Identity

Monday, November 10, 2014

Dr. Bruce Budowle

Massively Parallel (Next-Gen) Sequencing tools that meet forensic science needs and requirements for implementation are being developed. This webinar presents the results of studies using prototype NGS STR Multiplex System with Illumina's TruSeq™ DNA LT Sample Preparation Kit on the MiSeq™ system. These results demonstrate that this STR multiplex system can generate reliable STR profiles with a sensitivity that competes with current CE-based methods.

The 6 x 5 LC-MS/MS Peptide Reference Mix and Software Analysis Tool

Proteomics

Tuesday, October 14, 2014

Mike Rosenblatt, Ph.D.

This webinar introduces the 6 x 5 LC-MS/MS Peptide Reference Mix as a standard for performance assessment of both the LC and MS portions of your workflow. Also featured is a free software package for historical analysis of instrument performance.

A Real-Time Cytotoxicity Assay That Delivers More Relevant Data

Cell-Based Assays

Tuesday, September 09, 2014

Andrew L. Niles

This webinar describes how a new, real-time assay can be conveniently employed to reveal the kinetics and magnitude of response for any individual cytotoxic profile without the limitations of conventional approaches. When combined with value-added multiplex chemistries, these data can provide important information for defining mechanism of action.

Enhance Laboratory Efficiency Through Workflow Integration

Genomics

Tuesday, August 12, 2014

Chris Moreland, MBA

Are you or your lab looking for ways to (i) improve your genomic workflows, (ii) cut costs, and/or (iii) increase the consistency and reliability of your results? If so, please join this webinar to learn more about the new Maxwell® RSC automated benchtop nucleic acid purification system which is designed to enhance laboratory and workflow efficiency while also cutting total costs associated with nucleic acid extraction.

Monitoring Mass Spec Instrument Performance and Method Development with Whole Cell Human and Yeast Protein Reference Extracts

Proteomics

Tuesday, July 08, 2014

Sergei Saveliev, Ph.D.

To address the need for a complex protein reference material suitable for mass spectrometry analysis, we developed intact and pre-digested whole cell yeast and human protein extracts. This webinar will outline the development and testing of these reference extracts with emphasis on LC/MS compatibility, material reproducibility and amenability to efficient mass spec analysis as measured by high numbers of peptide and protein identifications.

Customized and Automated Solutions to Overcome Challenges in Your Lab

MDx

Tuesday, June 24, 2014

Kevin Kershner

Having issues with an automated DNA/RNA purification platform or trouble purifying amplifiable DNA/RNA from tough samples? If so, it’s highly likely Promega can develop a custom, automated chemistry solution for you. Attend this webinar to learn how our Field Support Scientists can work with you directly in your lab to develop the solution you need.

Monitoring Protein:Protein Interactions in Living Cells Using NanoBRET™ Technology

Cell-Based Assays

Tuesday, June 10, 2014

Dr. Danette L. Daniels

Current methodology geared towards identifying small molecule inhibitors of protein:protein interactions usually rely upon physical disruption of individual protein domains or fragments in vitro, which might not necessarily reflect the physiological context of the cell. In this webinar we present an improved BRET assay for monitoring protein:protein interactions in living cells with examples including higher order complexes.

The Fundamentals of CE-Based DNA Analysis and Related Interpretation Issues

Genetic Identity

Wednesday, June 04, 2014

Bruce R. McCord, Ph.D

As forensic laboratories advance to newer and more sensitive instrumentation, it is useful to review how low-level STR data is generated and interpreted. In this webinar, Dr. McCord focuses on the underlying instrumental and chemical factors that affect the appearance of low-level DNA samples and mixtures, and provides tips on how to appropriately address these challenges.

Making Ultrasensitive Endogenous NanoLuc® Luciferase Reporter Cell Lines Using CRISPR

Drug Discovery

Tuesday, May 20, 2014

Eric Rhodes, Horizon Discovery Group, UK

Gene editing techniques like CRISPR hold great promise in moving from over expression systems to actual monitoring of endogeneous transcript levels. This webinar will discuss the utility of a small, extremely bright luciferase as a means of monitoring endogeneous transcript levels as well as discussing relevant points to consider when building such model systems.

Customized and Automated Solutions to Overcome Challenges In Your Lab

Genomics

Tuesday, May 13, 2014

Kevin Kershner

Having issues with an automated DNA/RNA purification platform or trouble purifying amplifiable DNA/RNA from tough samples? If so, it’s highly likely Promega can develop a custom, automated chemistry solution for you. Attend this webinar to learn how our Field Support Scientists can work with you directly in your lab to develop the solution you need.

A Fusion Tag for Complete Protein Characterization

Proteomics

Tuesday, April 08, 2014

Rob Chumanov, Ph.D., MBA

Learn about a Promega Technology that is used for protein purification, functional imaging, and protein-protein interaction discovery work. This webinar will compare our HaloTag® Technology to traditional approaches including fluorescent proteins, GST, HIS and Flag Tags. We will also provide simple approaches for getting started with the technology platform.

Reliable PCR Components for Molecular Diagnostic (MDx) Assays

MDx

Tuesday, March 25, 2014

Terri McDonnell, MBA, PMP

Introducing the new family of cGMP-manufactured GoTaq® MDx DNA Polymerase and Promega's growing portfolio of MDx products designed for use as components of PCR-based assays.

Overview of 3D Cell Culture Model Systems and Factors to Consider When Choosing and Validating Cell-Based Assays for Use With 3D Cultures

Cell-Based Assays

Tuesday, March 11, 2014

Terry Riss, Ph.D.

This webinar will describe an overview of various 3D cell culture model systems including advantages and disadvantages of each model. Also described will be examples of cell-based assays originally designed for monolayer cultures with optimized protocols for use with 3D cultures.

Dealing with Reverse Transcription and Amplification Inhibitors: Reagent Choice Matters

Genomics

Tuesday, February 11, 2014

Rod Pennington, Ph.D.

Depending on the nucleic acid purification method, purified nucleic acids may contain any number of compounds with the potential to affect downstream reactions such as reverse transcription or PCR amplification. With the goal of helping you improve these enzymatic reactions, we will discuss the various types and sources of potential inhibitors and their potential impacts including data contrasting the performance of different downstream reagents in the presence of inhibitors.

In Vitro, Cell-Free Protein Expression - How It Helps Speed Up Your Research

Proteomics

Tuesday, January 14, 2014

Gary Kobs

In vitro, cell-free protein expression is a convenient method for rapidly producing protein in 1-4 hours. This webinar will provide an overview of the various cell-free expression systems available and methods to label and detect the expressed proteins. We will illustrate use of the in vitro expressed proteins with an emphasis on the characterization of protein:protein and protein:DNA interactions.

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