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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. All presentations will be given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

It's All in the Details (or Small RNA): Simplified and Improved miRNA Purification from Tissue

MDx

Tuesday, January 27, 2015

Douglas Horejsh, Ph.D.

This webinar will discuss the growing field of miRNA research, the roles miRNA play in cells and a new solution for miRNA purification that simplifies purification without the use of any organic pre-processing.

Validation Studies on DNA Mixtures Using the PowerPlex® Fusion 6C System

Genetic Identity

Thursday, February 05, 2015

Dr. Antonio Alonso Alonso

This presentation will describe the validation studies conducted with the PowerPlex® Fusion 6C System using an Applied Biosystems® 3500 Genetic Analyzer for fragment detection, with special emphasis on the analysis of DNA mixtures. DNA mixture analysis included two DNA mixture series of female (major component)/male (minor component) and 15 body fluid mixed samples from previous GEDNAP (http://www.gednap.de) and GHEP-ISFG (http://www.gep-isfg.org) proficiency exercises.

An Innovative System for Improving DNA Isolation from Diverse Plant Species

Genomics

Tuesday, February 10, 2015

Chris Moreland and Dr. Jackson Moeller

The extraction of genomic DNA from plants often involves tedious manual methods that produce suboptimal gDNA yields and purity. This webinar will introduce a new automated chemistry for the extraction of gDNA from plants that overcome the hassles of manual purification while dramatically increasing yields and purity.

Complete FcγRIIIa Effector Function Analysis for mAb Biologics with High (V158) and Low (F158) Affinity Reporter Bioassays

Drug Discovery

Tuesday, February 24, 2015

Richard Somberg, Ph.D.

Promega has developed a pair of reporter-based ADCC assays that quantitatively measure Fc effector function for therapeutic antibodies using ADCC as MOA. We will discuss the benefits of using frozen, thaw-and-use cells for convenience, time and cost savings, and improved run-to-run assay variation.We will discuss the ease of use of the assays and how they can provide precise, accurate and reproducible data.

A Next-Generation Dual-Luciferase Reporter Assay Using Firefly and NanoLuc® Luciferase

Cell-Based Assays

Tuesday, March 10, 2015

Christopher T. Eggers, Ph.D.

This webinar introduces a new homogeneous dual reporter assay for firefly and NanoLuc(R) luciferases that offers greatly improved sensitivity, versatility, and ease-of-use.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Getting to “DNA-free”: The Quest for Forensic-Grade Certification

Genetic Identity

Thursday, November 14, 2013

Rohaizah James, Ph.D.

In this webinar, we discuss the proposed ISO/CD 18385 guidelines for minimizing the risk of human DNA contamination events in the manufacturing process, as well as proposed pass/fail parameters for Forensic DNA Grade certification.

To NanoDrop® or Not to NanoDrop®: Choosing the Most Appropriate Method for Nucleic Acid Quantitation

Genomics

Tuesday, November 12, 2013

Douglas Wieczorek, Ph.D.

Success or failure in DNA/RNA analysis applications often comes down to whether or not the appropriate amount of input nucleic acid is used, but nucleic acid quantitation methods reveal different information about a sample. This webinar will review absorbance, fluorescent nucleic acid-binding dyes and qPCR quantitation methods and present the advantages and disadvantages of each method. With this information, you'll be able to choose the appropriate quantitation method based on your sample type and downstream application.

Overcoming Key Challenges of Protein Mass Spectrometry Sample Preparation with ProteaseMAX™ Surfactant

Proteomics

Tuesday, October 15, 2013

Dr. Sergei Saveliev

Surfactants such as SDS and Triton X-100 are commonly used during mass spec protein sample preparation, but these compounds are incompatible with reverse phase chromatography and mass spec and must be removed before use. This webinar will cover an easy-to-use mass spec-compatible surfactant that does not require removal prior to analysis. We will demonstrate its use and advantages for in-gel protein digestion, re-solubilization of protein pallets and other key mass spec protein sample preparation steps.

Improving Genetic Analysis: From FFPE Tissue DNA Extraction to MSI Analysis

MDx

Tuesday, September 24, 2013

Rebecca Gorshe

Many molecular genetic analyses start with DNA extracted from FFPE samples, but extraction of amplifiable DNA from these samples is difficult. This webinar will discuss methods to improve molecular genetic analysis starting with FFPE samples. Read additional information below.

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