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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for therapeutic antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

Use of ADCC Reporter Bioassays in Influenza Vaccine Development

Drug Discovery

Tuesday, May 10, 2016

Dr. Harmjan Kuipers, Janssen Pharmaceutical, Infectious Diseases and Vaccines

This webinar will discuss the application of Promega’s ADCC reporter bioassays in influenza vaccine development with special emphasis on the setup and use and of a murine FcγRIV version of this assay.

RNA-Based Gene Fusion Detection: Next Generation Sequencing Applications and Sample Workflow Improvements

Genomics

Tuesday, April 26, 2016

Joshua Stahl, M.Sc., ArcherDx, Inc.

This webinar will detail the advantages of gene fusion detection via RNA-Seq methods and enhancements that can be gained through improvements in nucleic acid purification protocols.

SoftGenetics GeneMarker®HID: A User’s Perspective

Genetic Identity

Wednesday, April 20, 2016

Jessica Seifarth

SoftGenetics introduced themselves to the forensic DNA community by offering a new alternative in STR analysis, GeneMarker®HID. Since their initial launch, GeneMarker®HID has been gaining traction by continuously developing new advancements. Papers have already been published illustrating the software’s capabilities of generating accurate and reliable results. This webinar will focus on a user’s capabilities within the GeneMarker®HID software. The ultimate goal is to illustrate the ease of use, benefits, and additional features for a forensic DNA analyst.

Bioactivity Assays: Putting the Puzzle Together

Drug Discovery

Tuesday, April 12, 2016

Ulrike Herbrand, PhD, Charles River Laboratories

In past years, the requirement for bioactivity testing changed from later phase lot release and stability testing to additional applications like biocomparability testing for follow-on biologics, accelerated stress condition testing and confirmation of successful production scale up. Therefore a suitable bioassay is needed at a very early time point. This webinar will look at a wide variety of therapeutic proteins and illustrate some best practices in method development that results in reliable, reproducible and precise assays, and discuss which assays may be best for your product and development program.

Isolation of Circulating Tumor DNA from Archived Plasma Samples for Downstream Digital Droplet PCR and Next Generation Sequencing Approaches

Genomics

Tuesday, March 22, 2016

Andrew Benjamin Nixon, PhD, MBA, Duke University School of Medicine

This webinar will explore circulating cell-free DNA workflows for biomarker discovery and downstream NGS analysis of tumor samples in a research environment.

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