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Promega Webinars

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Welcome to the Promega Webinar Series, an ongoing communication designed to keep you informed. Learn about basic concepts, tips and techniques to help your research, or understand how products were designed and how to implement in your lab. Most presentations are given by Promega scientists and you will have an opportunity to interact with our Technical Service Scientist directly during live events via chat. The webinars are free though we do ask that you register for the events. Registration allows us to send you the URL for the webinar.

MicroRNA Analysis Paired with Novel Cell Health Assays: A Complete Workflow

Genomics

Tuesday, February 28, 2017

Brad Hook, PhD

MicroRNAs (miRNAs) are small-noncoding RNA molecules that regulate gene expression post-transcriptionally. Amplification and overexpression of individual oncomiRs (miRNAs associated with cancer) or genetic loss of tumor suppressor miRNAs are associated with human cancer and are sufficient to drive tumorigenesis in mouse models. A global decrease in miRNA levels has been observed in human cancers and linked to genetic and epigenetic alterations in components of the miRNA biogenesis machinery. Learn more about how to analyze miRNAs in human cancer cells as we present data on a two studies that use new novel assays to monitor cell health and purify total RNA (including miRNAs) from the same cells.

Factors to Consider for Designing and Optimizing Assays Applied to 3D Cultures

Cell-Based Assays

Tuesday, March 14, 2017

Terry Riss, PhD

3D cell culture models present a challenge for using assay chemistries and imaging methods that were originally designed for measuring events from 2D monolayers of cells. In this webinar, we will present factors to consider when designing and optimizing assays applied to 3D cell culture models, case studies describing design of reagents to overcome the limitations inherent with detecting markers from large 3D culture models, and applications of novel real-time detection methods.

Optimizing and Qualifying Reporter Gene Bioassays for Immune Checkpoint Receptors

Drug Discovery

Tuesday, March 28, 2017

Zhijie Jey Cheng, PhD

Reporter gene assays for functional screening and development of antibody drug candidates are gaining popularity due to the significant advantages they offer over traditional methods that rely on human primary immune cells. We will discuss development of a series of MoA-based reporter gene bioassays for therapeutic antibodies targeting co-inhibitory receptors, co-stimulatory receptors and combination bioassays that target immune checkpoint receptors.

Post-Translational Modification Enzymes: How to Interrogate Key Drug Targets

Cell-Based Assays

Tuesday, April 11, 2017

Hicham Zegzouti, PhD

Post-translational modifications (PTMs), the addition of functional groups to proteins, play a significant role in regulating cellular biology. Examples include phosphorylation, a common mechanism in regulating enzymatic activity, and the addition of carbohydrates to many eukaryotic proteins, which promotes proper folding, improves stability and serves in regulatory functions. A key objective of research and drug discovery surrounding PTMs is to study the regulation of these enzymes and find specific modulators of their activity. Although mass spectrometry, eastern and western blotting detection technologies have been used traditionally, none are amenable to high throughput or as sensitive as bioluminescent detection of nucleotide-based substrates for such enzymes.

Did you miss one of our webinars? Simply select the appropriate link below and view the recorded webinar. It will not be interactive, but you will see the chat questions the original attendees asked. For additional information on the products discussed in the webinar, explore our links to videos and other resources.

If there is an area you would like to see covered, you can request a topic of your choice.

If you are experiencing issues opening the webinar recordings, please be sure that you have the latest Adobe Flash Player installed.

What Factors Do You Need to Consider When Bringing Automation Into Your Lab?

Genomics

Tuesday, July 26, 2016

Shaun Peterson

Considering laboratory automation? Successful automation projects can increase efficiency by reducing failures, eliminating protocol bottlenecks and eliminating tedious tasks freeing lab members for other activities. Learn about laboratory automation, considerations for choosing automation and how Promega can assist you in automating nucleic acid extraction and downstream applications in your laboratory.

Streamline Your Antibody Enrichment Using Scalable Magnetic Bead-Based Chemistries

Proteomics

Tuesday, July 12, 2016

Samantha Lewis, PhD

Learn about scalable magnetic bead-based antibody enrichment options to increase efficiency and throughput for antibody purification, conjugation, and pharmacokinetic studies.

Advances in Genetically Modified Food and Ingredient Authentication Testing

Genomics

Tuesday, June 28, 2016

Hemanth Shenoi, PhD

Join us for this webinar that will highlight current and advanced techniques for the detection of genetically modified (GM) events in food and food ingredients and the importance of high quality DNA to the process.

Live-Cell Quantification of Target Engagement and Compound Residence Time Using a Novel BRET Assay

Cell-Based Assays

Tuesday, June 14, 2016

Matthew Robers

This webinar will present a novel BRET-based method for measuring target engagement that significantly increases the type of data (permeability, equilibrium affinity, and residence time) at intra-cellular kinase and epigenetic targets in a live cell context.

Tools to Improve Protein Sample Preparation for Mass Spec

Proteomics

Tuesday, May 24, 2016

Sergei Saveliev, PhD

This webinar will discuss common shortcomings of protein mass spec sample preparation and provide solutions to these shortcomings.

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