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Int. Congr. Ser. 1239, 609–11. Evaluation of Powerplex™ 16 for typing of degraded DNA samples. 2003

Glock, B., Reisacher, R.B.K.. Rennhofer, S.O., Tröscher, D., Dauber, E.M. and Mayr, W.R.

Notes: The authors evaluated the ability of the PowerPlex® 16 System to amplify degraded DNA samples and compared the results with those obtained using the AmpFlSTR® SGM Plus® kit. They amplified DNA from six whole blood samples that had been stored at 4°C or room temperature for 20 days, DNA isolated from six pairs of archived whole blood samples, and from corresponding plasma samples that had been stored below –20°C for 1–3 years and were assumed to be degraded. Amplifications were assembled and performed using protocols optimized in their laboratory. For the whole blood and plasma samples stored below –20°C, the PowerPlex® 16 System gave eight full profiles and the SGM Plus® kit gave six full profiles. No differences were observed between the two kits with DNA extracts stored at 4°C or room temperature. As expected, the frequency of allele dropout increased as the amplified fragment length increased. (3876)

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J. Forensic Sci. 47, 773-785. Validation of a 16-locus fluorescent multiplex system. 2003

Krenke, B.E., Tereba, A., Anderson, S.J., Buel, E., Culhane, S., Finis, C.J., Tomsey, C.S., Zachetti, J.M., Masibay, A., Rabbach, D.R., Amiott, E.A. and Sprecher, C.J.

Notes: In this paper, researchers from both Promega and state crime laboratories provide an evaluation of the Powerplex® 16 System loci (CODIS, Penta D, Penta E, and amelogenin). Data from several laboratories using a variety of thermal cyclers and the ABI PRISM® 310 Genetic Analyzer or ABI PRISM® 377 DNA Sequencer are presented. The Powerplex® 16 System was able to consistently genotype samples from as little as 0.0625-2ng of genomic DNA template. Other factors such as reaction volume, annealing temperature, titration of AmpliTaq Gold® DNA Polymerase, magnesium, or primer pairs, and 1ng DNA mixtures were analyzed using the Powerplex® 16 System.  The researchers also present data on stutter analysis and cross reactivity of primer sets on primate DNA templates for each loci. (2675)

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Nat. Biotechnol. 21(10), 1233-1237. Large-scale genotyping of complex DNA. 2003

Kennedy, G.C., Matsuzaki, H., Dong, S., Liu, W.M., Huang, J., Liu, G., Su, X., Cao, M., Chen, W., Zhang, J., Liu, W., Yang, G., Di, X., Ryder, T., He, Z., Surti, U., Phillips, M.S., Boyce-Jacino, M.T., Fodor, S.P. and Jones, K.W.

Notes: Terminal Deoxynucleotidyl Transferase was used to biotin end-label PCR products generated from adaptor-ligated genomic DNA fragment templates. The labeled probes were then hybridized to microarrays spotted with SNP alleles. The Terminal Deoxynucleotidyl Transferase reaction was performed for 4 hours at 37°C using 15-20 units TdT, TdT reaction buffer and 18μM biotin-ddATP. (2758)

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Int. Congr. Ser. 1239, 125–30. PowerPlex™ 16 analysis in the Japanese population. 2003

Hashiyada, M., Itakura, Y. and Nata, M.

Notes: The authors generated population data from 508 unrelated Japanese individuals using the PowerPlex® 16 System. DNA was collected as buccal swabs and isolated using Chelex® resin followed by phenol:chloroform extraction and ethanol precipitation. For each PowerPlex® 16 reaction, 2ng of DNA was amplified using a GeneAmp® PCR System 9700, and amplified products were detected using the ABI PRISM® 377 DNA Sequencer. (3843)

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J. Clin. Microbiol. 41, 3064–3069. Invasive disease due to nontypeable Haemophilus influenzae among children in Arkansas. 2003

O’Neill, J.M., St. Geme III, J.W., Cutter, D., Adderson, E.E., Anyanwu, J., Jacobs, R.F. and Schutze, G.E.

Notes: The Wizard® Genomic DNA Purification System was used to isolate genomic DNA from clinically isolated Haemophilus influenzae samples.  The genomic DNA was then digested with EcoRI and used in Southern blots to identify the presence of various Haemophilus influenzae adhesin genes. (3102)

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Nucl. Acids Res. 31 (7), e35. Light-directed 5´-->3´ synthesis of complex oligonucleotide microarrays. 2003

Albert, T.J., Norton, J., Ott, M., Richmond, T., Nuwaysir, K., Nuwaysir, E.F., Stengele, K.P. and Green, R.D.

Notes: The PolyATtract® System 1000 was used to isolate mRNA from mouse spleen. The researchers used 3μg of the isolated poly(A)+ RNA in reverse transcriptase reactions to make cDNAs. The cDNAs were ultimately used as templates in in vitro transcription reactions to produce biotin labeled cRNAs that were used as targets in microarray analysis experiments.  (2743)

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Croat. Med. J. 42(3), 260-266. DNA typing from skeletal remains: evaluation of multiplex and megaplex STR systems on DNA isolated from bone and teeth samples. 2001

Alonso, A., Andelinovic, S., Martin, P., Sutlovic, D., Erceg, I., Huffine, E., de Simon, L.F., Albarran, C., Definis-Gojanovic, M., Fernandez-Rodriguez, A., Garcia, P., Drmic, I., Rezic, B., Kuret, S., Sancho, M. and Primorac, D.

Notes: The authors report use of the PowerPlex® 16 System for STR analysis of DNA from skeletal remains. (2279)

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J. Forensic Sci. 46(1), 111-115. DNA typing results from two urban subpopulations of Pakistan. 2001

Rahman, Z., Afroze, T. and Weir, B.S.

Notes: The GenePrint® CSF1PO, TPOX, TH01 Multiplex (CTT) was used in this population study. With this system amplified products are detected by silver staining following denaturing polyacrylamide gel electrophoresis. (2269)

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J. Forensic Sci. 46(3), 736-761. Allele frequencies for fourteen STR loci of the PowerPlex™ 1.1. and 2.1 multiplex systems and Penta D locus in caucasians, african-americans, hispanics, and other populations of the United States of America and Brazil. 2001

Levedakou, E.N., Freeman, D.A., Budzynski, M.J., Early, B.E., McElfresh, K.C., Schumm, J.W., Amin, A.S., Kim, Y.K., Sprecher, C.J., Krenke, B.E., Silva, D.A., McIntosh, T.M., Grubb, J.C., Johnston, L.J., Sailus, J.S., Ban, J.D., Crouse, C.A. and Nelson, M.S.

Notes: This paper details allele frequencies for the loci contained in the PowerPlex™ 1.1 and 2.1 Systems. (2262)

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J. Forensic Sci. 46(3), 647-660. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. 2001

Moretti, T.R., Baumstark, A.L., Defenbaugh, D.A., Keys, K.M., Smerick, J.B. and Budowle B.

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex® Systems were evaluated, along with other commercially available STR typing systems. (2267)

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Mol. Diag. 6, 55-61. Analysis of the factor V Leiden mutation using the READIT Assay. 2001

Rhodes, R.B., Lewis, K., Shultz, J., Huber, S., Voelkerding, K.V., Leonard, D.G., Tsongalis, G.J. and Kephart, D.D.

Notes: A variety of methods exist for the detection of single-nucleotide polymorphisms (SNPs) present in amplified segments of genomic DNA. The authors show the application of Promega's READIT® SNP Genotyping System, a novel SNP scoring tool, for analysis of the factor V Leiden mutation. (2139)

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J. Clin. Microbiol. 38(8), 2829-2836. Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon 2000

Park, S., Wong, M., Marras, S. A. E., Cross, E.W., Kiehn, T.E., Chaturvedi, V., Tyagi, S., and Perlin, D.S.

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

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J. Forensic Sci. 45(4), 886-890. A novel approach to obtaining reliable PCR results from luminol treated bloodstains. 2000

Della Manna, A. and Montpetit, S.

Notes: In this study, the PowerPlex® 1.1 System was used for STR typing of DNA isolated from luminol treated bloodstains. (2252)

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J. Forensic Sci. 45(3), 677-683. Validation of the PowerPlex 1.1™ loci for use in human identification. 2000

Greenspoon, S.A., Lytle, P.J., Turek, S.A., Rolands, J.M., Scarpetta, M.A. and Carr, C.D.

Notes: This paper describes validation of Promega's PowerPlex® 1.1 System. (2259)

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Forensic Sci. Int. 129, 43–50. A report of the 2000 and 2001 paternity testing workshops of the English Speaking Working Group of the International Society for Forensic Genetics. 2000

Hallenberg, C. and Morling, N.

Notes: These authors present the results of the 2000 and 2001 Paternity Testing Workshops, which were held to compare DNA typing results and laboratory practices between laboratories. Participating labs received a questionnaire about their processes and blood samples for DNA analysis. The results were compiled, and 91% (2000) and 86% (2001) of labs used PCR-based methods for DNA typing, and typing errors occurred in 0.3% (2000) and 0.1% (2001) of the submitted results. The PCR-based STR-typing kits used in this study included the PowerPlex® 16 System and F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein). (3834)

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Appl. Environ. Microbiol. 65, 2369-2375. High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting. 1999

Duim, B., Wassenaar, T.M., Rigter, A., Wagenaar, J.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 109 cells/ml. The isolated DNA was used for AFLP analysis. (1203)

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Genetics 151, 785-795. Multiple-trait quantitative trait loci analysis using a large mouse sibship. 1999

Jackson, A.U., Fornes, A., Galecki, A., Miller, R.A., Burke, D.T.

Notes: 20ng mouse genomic DNA was amplified with primers specific for 83 SSLP (single sequence length polymorphism) marker loci for genome-wide genotyping. Amplification products were separated on a denaturing gel and were visualized by silver staining using the SILVER SEQUENCE™ DNA Sequencing System. (0965)

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Am. J. Hum. Genet. 62, 599-609. Identification of novel mutations in the ryanodine-receptor gene (RYR1) in malignant hyperthermia: genotype-phenotype correlation. 1998

Manning, B. M. , Quane, K. A. , Ording, H. , Urwyler, A. , Tegazzin, V. , Lehane, M. , O'Halloran, J. , Hartung, E. , Giblin, L. M. , Lynch, P. J. , Vaughan, P. , Censier, K. , Bendixen, D. , Comi, G. , Heytens, L. , Monsieurs, K. , Fagerlund, T. , Wolz, W. , Heffron, J. J. , Muller, C. R. , McCarthy, T. V.

Notes: First-strand cDNA was synthesized by use of the Reverse Transcription System and random primers. (0728)

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Biotechniques 23, 742-747. Multiplex systems for the amplification of short tandem repeat loci: Evaluation of laser fluorescence detection. 1997

Ricciardone, M.S., Lins, A.M., Schumm, J.W.and Holland, M.M.

Notes: In this study, amplification products obtained using two fluorescent multiplex systems (the CSF1PO, TPOX, TH01, vWA Multiplex and the F13A01, FESFPS, F13B, LPL Multiplex) were analyzed using 3 different detection instruments. Concordant results were obtained with all three detection methods. (2270)

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J. Forensic Sci. 42(4), 701-707. Validation studies of the CTT STR multiplex system. 1997

Budowle, B., Moretti, T.R., Keys, K.M., Koons, B.W. and Smerick, J.B.

Notes: This validation study evaluated the utility of the GenePrint® CSF1PO, TPOX, TH01 Multiplex (Silver Stain Detection) for forensic casework analyses. (2289)

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Proc. Natl. Acad. Sci. USA 94, 10273-10278. DNA typing in thirty seconds with a microfabricated device. 1997

Schmalzing, D., Koutny, L., Adourian, A., Belgrader, P., Matsudaira, P. and Ehrlich, D.

Notes: In this paper the GenePrint® CSF1PO, TPOX, TH01, vWA Multiplex (Fluorescein) (CTTv) was used to amplify samples that were subsequently analyzed using a microfluidic electrophoresis device. (2255)

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J. Forensic Sci. 41(1), 155-162. Using multiplex PCR amplification and typing kits for the analysis of DNA evidence in a serial killer case. 1996

Hochmeister, M.N., Budowle, B., Eisenberg, A., Borer, U.V. and Dirnhofer, R.

Notes: This is the first report of the use of commercially available STR typing kits for the analysis of DNA evidence in a serial killer case and the analysis has been admitted in court. The GenePrint® CSF1PO, TPOX, TH01 Multiplex (silver stain detection) was one of the str typing systems used. (2261)

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Br. J. Haematol. 89(3), 633-636. Non-radioactive SSCP for genotyping human platelet alloantigens. 1995

Peyruchaud, 0, Nurden, A, Bourre, F.

Notes: Promega's Taq DNA Polymerase was used in this study. (1957)

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J. Forensic Sci. 40(4), 701-705. Confirmation of the identity of human skeletal remains using multiplex PCR amplification and typing kits. 1995

Hochmeister, M.N., Budowle, B., Borer, U.V., Rudin, O., Bohnert, M. and Dirnhofer, R.

Notes: This is the first report of a case using commercially available multiplex STR kits to confirm the identity of skeletal remains. One of the systems used was the GenePrint® CSF1PO, TPOX, TH01 Multiplex (silver stain detection). (2260)

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