Notes: A variety of methods exist for the detection of single-nucleotide polymorphisms (SNPs) present in amplified segments of genomic DNA. The authors show the application of Promega's READIT^® SNP Genotyping System, a novel SNP scoring tool, for analysis of the factor V Leiden mutation. (2139)

Notes: The authors report use of the PowerPlex^® 16 System for STR analysis of DNA from skeletal remains. (2279)

Notes: The GenePrint^® CSF1PO, TPOX, TH01 Multiplex (CTT) was used in this population study. With this system amplified products are detected by silver staining following denaturing polyacrylamide gel electrophoresis. (2269)

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex^® Systems were evaluated, along with other commercially available STR typing systems. (2267)

Notes: These authors present the results of the 2000 and 2001 Paternity Testing Workshops, which were held to compare DNA typing results and laboratory practices between laboratories. Participating labs received a questionnaire about their processes and blood samples for DNA analysis. The results were compiled, and 91% (2000) and 86% (2001) of labs used PCR-based methods for DNA typing, and typing errors occurred in 0.3% (2000) and 0.1% (2001) of the submitted results. The PCR-based STR-typing kits used in this study included the PowerPlex® 16 System and F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein). (3834)

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard^® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

Notes: The Wizard^® Genomic DNA Purification Kit was used to isolate genomic DNA from Campylobacter sp. Prior to isolation, the bacteria were washed in TE and resuspended in 1ml of TE at ~1 x 10⁹ cells/ml. The isolated DNA was used for AFLP analysis. (1203)

Notes: First-strand cDNA was synthesized by use of the Reverse Transcription System and random primers. (0728)

Notes: This validation study evaluated the utility of the GenePrint^® CSF1PO, TPOX, TH01 Multiplex (Silver Stain Detection) for forensic casework analyses. (2289)

Notes: Promega's Taq DNA Polymerase was used in this study. (1957)