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Promega Corporation

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J. Forensic Sci. 46(3), 647-660. Validation of short tandem repeats (STRs) for forensic usage: performance testing of fluorescent multiplex STR systems and analysis of authentic and simulated forensic samples. 2001

Moretti, T.R., Baumstark, A.L., Defenbaugh, D.A., Keys, K.M., Smerick, J.B. and Budowle B.

Notes: In this study, the amplification and typing conditions for the 13 core CODIS loci and their forensic applicability were evaluated. These loci are CSF1PO, FGA, TH01, TPOX, vWA, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, and D21S11. The PowerPlex® Systems were evaluated, along with other commercially available STR typing systems. (2267)

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Forensic Sci. Int. 124(1), 47-54. STR primer concordance study. 2001

Budowle, B., Masibay, A., Anderson, S.J., Barna, C., Biega, L., Brenneke, S., Brown, B.L., Cramer, J., DeGroot ,G.A., Douglas, D., Duceman, B., Eastman, A., Giles, R., Hamill, J., Haase, D.J., Janssen, D.W., Kupferschmid, T.D., Lawton, T., Lemire, C., Llewellyn, B., Moretti, T., Neves, J., Palaski, C., Schueler, S., Sgueglia, J., Sprecher, C., Tomsey, C. and Yet, D.

Notes: This study reports that the primers used in the PowerPlex® 16 System are reliable for typing reference samples that are used in the FBI CODIS database. The authors also report allele frequency data for the Penta D and Penta E loci. (2275)

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J. Forensic Sci. 46(4), 998-999. Allele frequencies for the PowerPlex® 16 STR loci in an Albanian population sample from northern Italy. 2001

Robino, C., Gino, S. and Torre, C.

Notes: The authors performed a population study using the PowerPlex® 16 System. (2277)

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J. Forensic Sci. 45(6), 1360-1362. Promega Corporation reveals primer sequences in its testing kits. 2000

Masibay, A., Mozer, T.J. and Sprecher, C.

Notes: This paper reveals the primer sequence for Promega's PowerPlex 1.1, amelogenin, FFFL and PowerPlex 1.2 Systems. (2265)

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J. Forensic Sci. 45(3), 677-683. Validation of the PowerPlex 1.1™ loci for use in human identification. 2000

Greenspoon, S.A., Lytle, P.J., Turek, S.A., Rolands, J.M., Scarpetta, M.A. and Carr, C.D.

Notes: This paper describes validation of Promega's PowerPlex® 1.1 System. (2259)

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Electrophoresis 21(2), 347-350. Short tandem repeat analysis in Japanese population. 2000

Hashiyada, M.

Notes: This paper describes a Japanese population study of the loci contained in the PowerPlex 1.1 and 2.1 Systems. (2287)

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Am. J. Forensic. Med. Pathol. 21(2), 97–100. Isolation and identification of female DNA on postcoital penile swabs. 2000

Cina, S.J., Collins, K.A., Pettenati, M.J. and Fitts, M.

Notes: In this study, DNA isolates were typed using the PowerPlex® 1.1 System. (2286)

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Arch. Pathol. Lab. Med. 124, 1083–1086. Isolation and identification of male and female DNA on a postcoital condom. 2000

Cina, M.S., Collins, K.A., Fitts, M. and Pettenati, M.J.

Notes: The authors swabbed internal and external surfaces of a postcoital codom to screen for the presence of female cells and isolated DNA from the swabs using standard organic extraction. The PowerPlex® 1.1 System was used to amplify 8 STR loci plus amelogenin and identify the source of DNA from both surfaces. Amplification products were detected using a Hitachi FMBIO® II fluorescence detection system. Only female DNA was detected on the external surface, and only male DNA was detected on the internal surface. (2285)

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Forensic Sci. Int. 129, 43–50. A report of the 2000 and 2001 paternity testing workshops of the English Speaking Working Group of the International Society for Forensic Genetics. 2000

Hallenberg, C. and Morling, N.

Notes: These authors present the results of the 2000 and 2001 Paternity Testing Workshops, which were held to compare DNA typing results and laboratory practices between laboratories. Participating labs received a questionnaire about their processes and blood samples for DNA analysis. The results were compiled, and 91% (2000) and 86% (2001) of labs used PCR-based methods for DNA typing, and typing errors occurred in 0.3% (2000) and 0.1% (2001) of the submitted results. The PCR-based STR-typing kits used in this study included the PowerPlex® 16 System and F13A01, FESFPS, F13B, LPL Multiplex (Fluorescein). (3834)

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J. Forensic Sci. 44(6), 1243-1256. TWGDAM validation of a nine-locus and a four-locus fluorescent STR multiplex system. 1999

Micka KA, Amiott EA, Hockenberry TL, Sprecher CJ, Lins AM, Rabbach DR, Taylor JA, Bacher JW, Glidewell DE, Gibson SD, Crouse CA, Schumm JW

Notes: This paper describes validation of the PowerPlex® 1.1 System, Amelogenin and the FFFFL Fluorescent STR Systems according to the recommendations of the Technical Working Group on DNA Analysis Methods (TWGDAM). (2266)

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Electrophoresis 19(1), 76-79. Sex determination of forensic samples by polymerase chain reaction of the amelogenin gene and analysis by capillary electrophoresis with polymer matrix. 1998

Pouchkarev, V.P., Shved, E.F. and Novikov, P.I.

Notes: The GenePrint® Sex Identification System-Amelogenin was used in combination with a capillary electrophoresis system to correctly identify subnanogram quantities of male and female DNA. (2288)

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Proc. Natl. Acad. Sci. USA 94, 10273-10278. DNA typing in thirty seconds with a microfabricated device. 1997

Schmalzing, D., Koutny, L., Adourian, A., Belgrader, P., Matsudaira, P. and Ehrlich, D.

Notes: In this paper the GenePrint® CSF1PO, TPOX, TH01, vWA Multiplex (Fluorescein) (CTTv) was used to amplify samples that were subsequently analyzed using a microfluidic electrophoresis device. (2255)

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Proceedings from First European Symposium on Human Identification , 90-104. Automated fluorescent detection of STR multiplexes - development of the GenePrint® PowerPlex® and FFFL Multiplexes for forensic and paternity applications. 1997

Schumm, J., Becker, S. and Sablowski, T.

Notes: The authors discuss development of the GenePrint® PowerPlex® and FFFL Multiplexes for forensic and paternity applications. (2247)

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