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Forensic Sci. Int. 146 Suppl., S129–S131. Genetic study of 11 Y-STRs in the populations of Reggio Calabria, Catanzaro, Cosenza (Calabria--South of Italy). 2004

Barbaro, A., Cormaci, P., Falcone, G., Barbaro, A. and Rizzo, M.

Notes: Blood samples were collected from approximately 300 males in southern Italy. DNA was extracted, quantified using the AluQuant® Human DNA Quantitation System, and amplified using the PowerPlex® Y System. Amplification products were detected using the ABI PRISM® 310 genetic analyzer. The distribution of Y-STR haplotypes in three southern Italian populations was determined. (3639)

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Minerva Ginecol. 56(3), 189-96. Intracytoplasmic sperm injection. Accomplishments and qualms. 2004

Neri, Q.V., Tanaka, N., Wang, A., Katagiri, Y., Takeuchi, T., Rosenwaks, Z. and Palermo, G.D.

Notes: These authors assessed the risk of transmitting genetic defects to children after intracytoplasmic sperm injection (ICSI) for treatment of male factor infertility. Genomic DNA was isolated from blood of both parents and children using the Wizard® Genomic DNA Purification Kit. Multiplex amplification of 22 sequence tagged sites on the Y chromosome was then performed on 123 samples using the Y Chromosome Deletion Detection System, Version 1.1 and a prototype Multiplex E from Promega. (3117)

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Forensic Sci. Int. 134, 219–221. STR data for PowerPlex 16 System from Neuquen population, SW Argentina. 2004

Toscanini, U., Berardi, G. and Raimondi, E.

Notes: One hundred and eleven people from the Neuquen province in Argentina were typed with the PowerPlex™ 16 System. Allele frequencies for the population are provided. (3009)

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J. Forensic Sci. 49, 71–80. Validation and implementation of the PowerPlex™ 16 BIO System STR multiplex for forensic casework. 2004

Greenspoon, S.A., Ban, J.D., Pablo, L., Crouse, C.A., Kist, F.G., Tomsey, C.S., Glessner, A.L., Mihalacki, L.R., Long, T.M., Heidebrecht, B.J., Braunstein, C.A., Freeman, D.A., Soberalski, C., Bruesehoff, N., Amin, A.S., Elizabeth K. Douglas E.K. and Schumm, J.W.

Notes: A concordance study was performed by several independent agencies using the PowerPlex® 16 BIO System. The study tested the ability of the PowerPlex® 16 BIO System to type 653 samples previously typed using the PowerPlex® 1.1 and 2.1 Systems. The authors discuss stutter, sensitivity and precision. Studies were also performed with mixed samples, nonhuman DNA samples, and samples exposed to various environmental conditions. (3008)

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Int. Congr. Ser. 1239, 165–9. Allele distribution of 15 STRs in a population from Extremadura (Central-Western Spain). 2003

García-Hirschfeld, J. Farfán, M.J., Prieto, V., López-Soto, M., Torres, Y. and Sanz, P.

Notes: The authors generated population data from unrelated individuals in Extremadura in western Spain using the PowerPlex® 16 System and the AmpFlSTR® Profiler Plus™, COfiler® and SGM Plus® kits. DNA was collected as blood samples and isolated by phenol:chloroform extraction or with GFX genomic purification columns. Amplifications were performed as directed by the manufacturer, and amplified products were detected using an ABI PRISM® 310 Genetic Analyzer. (3844)

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Forensic Sci. Int. 135, 60-63. Allele frequencies for 15 STR loci in Van-Agri districts of the Eastern Anatolia region of Turkey. 2003

Cakir, A.H., Celebioglu, A., Altunbas, S. and Yardimci, E.

Notes: The PowerPlex® 16 System was used to type 116 individuals from the Van-Agri districts of the Eastern Anatolia region of Turkey. The study reports allele frequencies for this population as well as other statistical data. (2822)

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Forensic Sci. Int. 136, 86-88. Allelic frequencies at 12 STR loci in Colombian population. 2003

Benítez-Páez, A. and Reyes, H.O.

Notes: Allele frequencies for a Mestizo population in Columbia were determined with the PowerPlex® 16 System. DNA was purified using the Wizard® Genomic DNA Purification Kit.  (3003)

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Int. Congr. Ser. 1239, 609–11. Evaluation of Powerplex™ 16 for typing of degraded DNA samples. 2003

Glock, B., Reisacher, R.B.K.. Rennhofer, S.O., Tröscher, D., Dauber, E.M. and Mayr, W.R.

Notes: The authors evaluated the ability of the PowerPlex® 16 System to amplify degraded DNA samples and compared the results with those obtained using the AmpFlSTR® SGM Plus® kit. They amplified DNA from six whole blood samples that had been stored at 4°C or room temperature for 20 days, DNA isolated from six pairs of archived whole blood samples, and from corresponding plasma samples that had been stored below –20°C for 1–3 years and were assumed to be degraded. Amplifications were assembled and performed using protocols optimized in their laboratory. For the whole blood and plasma samples stored below –20°C, the PowerPlex® 16 System gave eight full profiles and the SGM Plus® kit gave six full profiles. No differences were observed between the two kits with DNA extracts stored at 4°C or room temperature. As expected, the frequency of allele dropout increased as the amplified fragment length increased. (3876)

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Int. Congr. Ser. 1239, 673–6. Evaluation of “in house” criteria for PCR-based analysis in immigration casework. 2003

van Eede, P.H., Keller, S. and de Lange, G.G.

Notes: The authors evaluated criteria for using STR analysis to resolve immigration cases by examining 156 one-parent child combinations and 105 two-parent child combinations from 28 families. STR analyses were performed in duplicate using the SMG Plus® kit and a combination of CTTv and FFFL Multiplexes. The authors concluded that the combination of these kits, which analyze a total of 21 STR loci, can be used to successfully resolve immigration casework. However, in 9% of the one-parent cases their criteria of a parental index of 1.000 or more was not met. Additional testing using the PowerPlex® 16 System reduced this from 9% to 1 out of 156 indices. (3858)

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Forensic Sci. Int. 133, 254-255. Genetic data on 11 STRs (CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317, F13B, LPL) in an Argentine northeast population. 2003

Martinez, G., Vazquez, E., Schaller, C. and Quevedo, N.

Notes: The GenePrint® STR CSF1PO, TPOX, TH01 Multiplex,   F13A01, FESFPS, vWA Multiplex and SilverSTR® III System (D7S820, D13S317, D16S539) were used to type 225 individuals from the Entre Rios State in Argentina. Allele frequencies and other statistical analysis are provided. (2824)

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Forensic Sci. Int. 135, 67-71. Genetic data on 12 STRs (F13A01, F13B, FESFPS, LPL, CSF1PO, TPOX, TH01, vWA, D16S539, D7S820, D13S317, D5S818) from four ethnic groups of Sao Paulo, Brazil. 2003

Bydlowski, S.P., de Moura-Neto, R.S., Soares, R.P., Silva, R., Debes-Bravo, A.A. and Morganti, L.

Notes: Nine hundred and sixteen individuals from four ethnic groups: European-derived, African-derived, Brazilian Mulattos and Asian-derived, were typed with the GenePrint® STR CSF1PO, TPOX, TH01 Multiplex,  F13A01, FESFPS, vWA Multiplex, and SilverSTR® III (D7S820, D13S317, D16S539) Systems.  Allele frequencies as well as statistics for each group are provided. (2825)

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Forensic Sci. Int. 134, 225-231. Genetic diversity in four tribal groups of western India: a survey of polymorphism in 15 STR loci and their application in human identification. 2003

Gaikwad, S. and Kashyap, V.K.

Notes: The PowerPlex™ 16 System was used to type 426 people from 4 tribal groups in Western India. The allele frequencies for the PowerPlex™ 16 System loci are provided for each of the tribal groups. (3011)

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Forensic Sci. Int. 132, 216-222. Genetic polymorphism revealed by 13 tetrameric and 2 pentameric STR loci in four Mongoloid tribal population 2003

Maity, B., Nunga, S.C. and Kashyap, V.K.

Notes: The PowerPlex® 16 System was used to genetically type 354 people from four distinct tribes (Hmar, Lai, Lusei, and Mara) in Northeastern India. Allele frequencies and population statistics are provided.  (3004)

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Forensic Sci. Int. 135, 64-66. Genetic profile of the Ecuadorian Mestizo population (Ecuador-South America) by using the Power Plex 16 System Kit. 2003

Gonzalez-Andrade, F., Sanchez, D. and Martinez-Jarreta, B.

Notes: The PowerPlex® 16 System was used to type 317 Mestizos in Ecuador.  Allele frequencies and statistical analysis of the data are provided. (2821)

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Int. Congr. Ser. 1239, 131–5. Multiplex STR genotyping: comparison study, population data and new sequence information. 2003

Alves, C., Gusmão, L., Pereira, L. and Amorim, A.

Notes: The authors generated allele frequency data for 100 unrelated individuals from North Portugal and 110 unrelated individuals from Mozambique using the PowerPlex® 16 System, GenePrint® CTTv Multiplex and Profiler Plus™ kit. Four inconsistencies in genotypes between the kits were detected, three for D5S818 and 1 for D8S1179, due to primer-binding site mutations. (3862)

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Int. Congr. Ser. 1239, 565–7. Mutation rates at 23 different short tandem repeat loci. 2003

Dauber, E.M., Bär, W., Klintschar, M., Neuhuber, F., Parson, W., Glock, B. and Mayr, W.R.

Notes: The authors examined almost 24,000 meioses to calculate the mutation rates at 23 STR loci, including CD4, CSF1PO, D2S1338, D3S1358, D5S818, D7S820, D8S1132, D8S1179, D12S391, D13S317, D16S539, D17S976, D18S51, D19S433, D21S11, F13A1, F13B, FES, FGA, SE33, TH01, TPOX and VWA. DNA was collected as blood samples or buccal swabs, isolated and amplified using the Powerplex™ 16 System, Profiler™, Profiler Plus™, COfiler™ or SGM Plus™ kit or singleplex amplification. Their results confirm that loci with long uninterrupted repeats have higher mutation rates. (3865)

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Forensic Sci. Int. 133, 250-253. Polymorphism of 17 STRs by multiplex analysis in Japanese population. 2003

Hashiyada, M., Itakura, Y., Nagashima, T., Nata, M. and Funayama, M.

Notes: The authors used the PowerPlex® 16 System to type 526 Japanese individuals. Allele frequencies for 13 CODIS loci were determined with the PowerPlex® 16 System and AmpFlSTR® Identifiler® kits. (2823)

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Forensic Sci. Int. 138, 116–118. Population data for 11 STR loci in northeast China Han. 2003

Zhanga, Y.J., Xub, Q.S. and Lee, J.B.

Notes: In this paper, researchers report allele frequencies for a population in Northeastern China. Data was generated using the PowerPlex® 16 System. DNA from volunteers was extracted using a phenol chloroform method. (3000)

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Forensic Sci. Int. 138, 116–8. Population data for 11 STR loci in northeast China Han. 2003

Zhang, Y.J., Xu, Q.S. and Lee, J.B.

Notes: Allele frequencies for 11 autosomal STR loci were determined in the Yan Bian region of China. Population data for nine STR loci were determined using the AmpFlSTR® Profiler Plus™ kit and for the Penta D and Penta E loci using the PowerPlex® 16 System. DNA was isolated using phenol:chloroform extraction, and 1ng was amplified as directed by the manufacturer. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3826)

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Int. Congr. Ser. 1239, 243–4. Population data from Chile using PowerPlex-16. 2003

Celis, R., Aguirre, E., Lorente, J.A and Jorquera, H.C.

Notes: The authors generated population data from 323 unrelated individuals in Chile using the PowerPlex® 16 System. All loci were shown to be highly polymorphic. (3864)

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Int. Congr. Ser. 1239, 121–2. Population genetic analysis in Hungarian populations using the Powerplex™ 16 system. 2003

Egyed, B., Füredi, S., Angyal, M., Balogh, I., Kalmár, L., Tordai, A., Woller, J. and Pádár, Z.

Notes: The authors generated population data for 723 individuals living in four regions of Hungary. DNA was collected as blood samples and amplified using the PowerPlex® 16 System. Amplified products were detected using the ABI PRISM® 377 DNA Sequencer or 310 Genetic Analyzer. (3866)

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Int. Congr. Ser. 1239, 235–8. Portuguese population data on two pentanucleotide STR loci Penta E and Penta D. 2003

Ribeiro, T., Viriato, L., Vieira-Silva, C., Cruz, C., Espinheira, R. and Geada, H.

Notes: The authors reported population data for the Penta E and Penta D loci in 160 Portuguese Caucasians, 19 Portuguese of African origin and 150 meioses. DNA was extracted from blood samples using Chelex® resin, then purified using the Wizard® DNA Clean-Up System. Amplifications were performed using the PowerPlex® 16 System, the SGM Plus® kit and the GeneAmp® PCR System 9600. Amplified products were detected using an ABI PRISM® 377 DNA Sequencer. (3845)

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Int. Congr. Ser. 1239, 125–30. PowerPlex™ 16 analysis in the Japanese population. 2003

Hashiyada, M., Itakura, Y. and Nata, M.

Notes: The authors generated population data from 508 unrelated Japanese individuals using the PowerPlex® 16 System. DNA was collected as buccal swabs and isolated using Chelex® resin followed by phenol:chloroform extraction and ethanol precipitation. For each PowerPlex® 16 reaction, 2ng of DNA was amplified using a GeneAmp® PCR System 9700, and amplified products were detected using the ABI PRISM® 377 DNA Sequencer. (3843)

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Forensic Sci. Int. 134, 222-224. STR data for PowerPlex 16 System from Buenos Aires population, Argentina. 2003

Berardi, G., Toscanini, U. and Raimondi, E.

Notes: The PowerPlex® 16 System was used to report allele frequencies for a population of people living in Capital Federal and in the Buenos Aires Metropolitan Area. (2999)

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Forensic Sci. Int. 135, 75-78. STR frequencies for the PowerPlex 16 System Kit in a population from Northeast Spain. 2003

Paredes, M., Crespillo, M., Luque, J.A. and Valverde, J.L.

Notes: The PowerPlex® 16 System was used to type 204 Caucasian individuals from Northeastern Spain.  Allele frequencies and statistical analyses of the data are provided. (2820)

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