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J. Cell Sci. 115, 1643-1649. Stem cell factor activates telomerase in mouse mitotic spermatogonia and in primordial germ cells. 2003

Dolci, S., Levati, L., Pellegrini, M., Faraoni, I., Graziani, G., Di Carlo, A. and Geremia, R.

Notes: RT-PCR was used to assess the response of mitotic spermatogonia to Kit1. Spermatogonia were cultured with and without Kit1 and RNA harvested after 24 hours. RT-PCR was performed in a two-step reaction using first the ImProm-II™ Reverse Transcriptase in a 20µl reaction. One microliter of the RT reaction was removed and amplified in a 50µl PCR using the PCR Master Mix. (2626)

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J. Biol. Chem. 278, 33474-33481. The Acrodermatitis enteropathica gene ZIP4 encodes a tissue-specific, zinc-regulated zinc transporter in mice. 2003

Dufner-Beattie, J., Wang, F., Kuo, Y.M., Gitschier, J., Eide, D. and Andrews, G.K.

Notes: Improm-II™ Reverse Transcriptase was used to make cDNAs of mouse ZIP4, a zinc metal ion transporter. The authors used 1μg of total RNA for the reverse transcription reaction. Some reverse  transcription reaction products were used to make probes for Northern blot analysis of expression in various tissues. Others were used to make mammalian expression constructs for transient transfection experiments. Details of each application are provided. (2722)

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J. Biol. Chem. 278, 5156-5162. Y-box-binding protein YB-1 mediates transcriptional repression of human alpha2(I) collagen gene expression by interferon-gamma. 2003

Higashi, K., Inagaki, Y., Suzuki, N., Mitsui, S., Mauviel, A., Kaneko, H. and Nakatsuka, I.

Notes: The Y-box-binding protein YB-1 was examined through the use of a reporter plasmid with wildtype and mutant putative Y-box binding sites. The binding sites were constructed in the pGL3 Basic Vector and measured using the Luciferase Assay System. Studies were performed in normal human dermal fibroblasts.  Expression of YB-1 was found to repress expression of the COL1A2 gene at the transcriptional level. Confirmation of this dose-dependent inhibition was through measurement of the steady-state mRNA levels by quantitative, real-time RT-PCR.  The reverse transcription portion of the quantitative, real-time RT-PCR reactions were performed with ImProm-II™ Reverse Transcriptase followed by a TaqMan-type quantitative PCR step. (2623)

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J. Virol. 76, 12032-12043. Baculorus lef-12 is not required for viral replication. 2002

Guarino, L.A. Mistretta, T.-A. and Dong, W.

Notes: RT-PCR was used to confirm the expression of the lef-12 gene at various times post-infection. The RT step was performed using ImProm-II™ Reverse Transcriptase in a 20µl reaction volume. One microliter of the RT reaction was amplified in a 50µl PCR amplification reaction using the PCR Master Mix. (2628)

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