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EMBO J. 19(12), 2946-2957. Phosphorylation status of the SCR homeodomain determines its functional activity: Essential role for protein phosphatase 2A,B'. 2000

Berry, M. and Gehring, W.

Notes: The Serine-Threonine Phosphatase Assay System was used to monitor dephosphorylation of two phosphopeptides derived from the SCR homeodomain. Dephosphorylation was performed using the catalytic subunit of the PP2A protein. Authors also used pSI for cloning, protein kinase A enzyme, PKA and PKC assays using biotinylated peptides (SignaTECT® Protein Kinase assay systems), anti-β-galactosidase for Drosophila embryo staining, and TNT® Quick Coupled Transcription/Translation systems to produce mutant and wild type protein for gel shifts. (2150)

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J. Biol. Chem. 275, 14360-14366. Protein kinase A associates with cystic fibrosis transmembrane conductance regulator via an interaction with Ezrin 2000

Sun, F., Hug, M.J., Bradbury, N.A., Frizzell, R.A.

Notes: Immunoprecipitates of CFTR become phosphorylated upon addition of a PKA catalytic subunit which is inhibitable by the Protein Kinase A Inhibitor Peptide. The SignaTECT® cAMP-Dependent Protein Kinase Assay System was used to measure PKA activity in immunoprecipitates of the CFTR protein. The activity was almost totally inhibited by the Protein Kinase A Inhibitor Peptide. PKA activity was four times higher with the anti-CFTR antibody precipitates than with a control antibody and addition of cAMP increased the amount of phosphorylation observed in the immunoprecipitates. Immunoprecipitates were prepared from Calu-3 human airway epithelia cells. (0318)

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J. Biol. Chem. 275, 13377-13385. Stimulation of protein kinase C modulates insulin-like growth factor-1-induced Akt activation in PC12 cells. 2000

Zheng, W.-H. , Kar, S. , Quirion, R.

Notes: The pretreatment of PC12 cells with 20µM U0126 MEK Inhibitor for 20min prior to phorbol ester and IGF-1 treatment blocked the phosphorylation of Akt kinase. To determine the effect of PKC inhibitors on PKC activity in the PC12 cells after phorbol ester stimulation, lysates were prepared from various cellular fractions and analyzed for PKC activity with the SignaTECT® PKC Assay System. Control values were obtained in the absence of the lipid activator solution and in the presence of the Myristoylated PKC Inhibitor Peptide. The greatest increase in PKC activity was found in the membrane fraction. (0058)

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Am. J. Physiol. 277, E316-E324. A unique mechanism of desensitization to lipolysis mediated by β3-adrenoceptor in rats with thermal injury. 1999

Ikezu, T., Yasuhara, S., Granneman, J.G., Kraemer, F.B., Okamoto, T., Tompkins, R.G., Martyn, J.A.J.

Notes: The SignaTECT® cAMP-Dependent Protein Kinase Assay System was used to measure PKA activity in adipose tissue. Good detail is provided for tissue preparation. (0993)

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Mol. Pharmacol. 55, 118-125. Daunorubicin- and mitoxantrone-triggered phosphatidylcholine hydrolysis: Implication in drug-induced ceramide generation and apoptosis. 1999

Bettaieb, A., Plo, I., Mansat-de Mas, V., Quillet-Mary, A., Levade, T., Laurent, G. and Jaffrezou, J.-P.

Notes: The SignaTECT® Protein Kinase C Assay System was used to measure PKC activity in extracts of U937 human monocytic leukemia cells in the presence or absence of different drugs. Good detail on the preparation of cell extracts is provided. (1442)

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J. Cell Biol. 147, 59-70. Neurotrophin regulation of beta-actin mRNA and protein localization with growth cones. 1999

Zhang, H.L., Singer, R.H., Bassell, G.J.

Notes: The SignaTECT® cAMP-Dependent Protein Kinase Assay System was used to monitor the PKA activity in primary neuronal cultures due to NT-3 stimulation. (0089)

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J. Biol. Chem. 274, 478-485. Nuclear extracts lacking DNA-dependent protein kinase are deficient in multiple round transcription. 1999

Woodard, R. L. , Anderson, M. G. , Dynan, W. S.

Notes: The SignaTECT® DNA-Dependent Protein Kinase Assay System was used to confirm the inhibitory effect of Wortmannin on the kinase activity of DNA-Dependent Protein Kinase (DNA-PK). (0152)

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Am. J. Physiol. 276, H786-H792. Proapoptotic effects of ANG II in human coronary artery endothelial cells: Role of AT1 receptor and PKC activation. 1999

Li, D., Yang, B., Philips, M.I., Mehta, J.L.

Notes: The SignaTECT® Protein Kinase C Assay System was used to determine PKC activity in extracts of human coronary artery endothelial cells. The cells were induced into apoptosis by treatment with angiotensin II which was inhibitable with PKC inhibitors. (0791)

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Eur. J. Biochem. 262, 95-101. Protein kinase C antagonizes pertussis-toxin-sensitive coupling of the calcitonin receptor to adenylyl cyclase. 1999

Shyu, J.-F., Zhang, Z., Hernandez-Lagunas, L., Camerino, C., Chen, Y., Inoue, D., Baron, R., Horne, W.C.

Notes: HEK cells expressing the C1a-calcintonin receptor were stimulated with either calcitonin or PMA following a 12-hour serum starvation. Cell lysates were prepared and assayed for protein kinase C activity with the SignaTECT® PKC Assay System. Excellent detail is provided for preparation of the cell lysates. (0402)

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J. Biol. Chem. 274, 22060-22064. Roles of replication protein A and DNA-dependent protein kinase in the regulation of DNA replication following DNA damage. 1999

Wang, Y., Zhou, X.-Y., Wang, H., Huq, M.S., Iliakis, G.

Notes: The SignaTECT® DNA-Dependent Protein Kinase Assay System was used to assess the amount of DNA-PK activity in HeLa and MO59J cells extracts. The method of extract preparation is referenced. An in vitro DNA replication system was prepared and DNA-Dependent Protein Kinase was added to MO59J extracts to investigate the affect of the enzyme on the replication. (0206)

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J. Neurosci. 19, 2455-2463. The mitogen-activated protein kinase pathway mediates extrogen neuroprotection after glutamate toxicity in primary cortical neurons. 1999

Singer, C.A., Figueroa-Masot, X.A., Batchelor, R.H., Dorsa, D.M.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess LDH release from primary cortical neurons following 24hr exposure to glutamate. Various compounds including 2.5S Nerve Growth Factor were assessed for their ability to block the glutamate-induced cytotoxicity. One of the neuroprotective factors, estrogen, was also examined for induction protein tyrosine kinase activity with the the SignaTECT® Protein Tyrosine Kinase Assay System. The induction of PTK activity peaked at 1min and returned to normal within 10min. (0367)

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J. Immunol. 161, 6054-6058. Cooperative activation of TCRs by enterotoxin superantigens. 1998

Niedergang, F. , Dautry Varsat, A. , Alcover, A.

Notes:  This study used the SignaTECT® Protein Tyrosine Kinase (PTK) Assay System to assay the catalytic activity of protein tyrosine kinases associated with anti-Vβ3 immunoprecipitates (Vβ 3 refers to the beta chain isoform of the T cell receptor). (0611)

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Proc. Natl. Acad. Sci. USA 95, 14066-14070. DNA end-joining catalyzed by human cell-free extracts. 1998

Baumann, P. and West, S.C.

Notes: The authors used the SignaTECT® DNA-Dependent Protein Kinase Assay System to assay for DNA-PK activity in a cell-free system derived from extracts of human lymphoblastoid cell lines. (1470)

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Nature 394, 700-704. DNA-dependent protein kinase acts upstream of p53 in response to DNA damage. 1998

Woo, R.A., McLure, K.G., Lees-Miller, S.P., Rancourt, D.E. and Lee, P.W.

Notes: Promega's SignaTECT® DNA-Dependent Protein Kinase Assay System was used in this study. (1962)

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Biochemistry 37, 9827-9835. Implication of DNA-dependent protein kinase in an early, essential, local phosphorylation event during end-joining of DNA double-strand breaks in vitro. 1998

Gu, X.-Y., Weinfeld, M.A., Povirk, L.F.

Notes: The ability of wortmannin to inhibit DNA-dependent phosphorylation was determined with the SignaTECT® DNA-PK Assay System. Rather than counting the individual SAM Membrane squares, the authors exposed the membrane to film for a graphic display of the inhibition. (1081)

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Am. J. Physiol. 275, H568-H576. Ox-LDL induces apoptosis in human coronary artery endothelial cells: role of PKC, PTK, bcl-2, and Fas. 1998

Li, D., Yang, B., Mehta, J.L.

Notes: The authors used the SignaTECT® Protein Kinase Assay System (PKC) and SignaTECT® Protein Tyrosine Kinase Assay System (PTK) to study kinase activity in human coronary artery endothelial cells. The Myristoylated PKC peptide inhibitor was used to study repression of PKC activity. (0792)

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Am. J. Respir. Cell Mol. Biol. 19, 129-142. Prolonged cell-cycle arrest associated with altered cdc2 kinase in monocrotaline pyrrole-treated pulmonary artery endothelial cells. 1998

Thomas, H.C., Lame, M.W., Morin, D., Wilson, D.W., Segall, H.J.

Notes: Authors use the SignaTECT® cdc2 Protein Kinase Assay System in their studies with Bovine pulmonary artery endothelial cells (BPAEC). (0260)

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J. Immunol. 161, 4819-4824. The role of protein kinase C signaling in activated DRA transcription. 1998

Setterblad, N., Onyango, I., Pihlgren, U., Rask, L., Andersson, G.

Notes: The authors used the SignaTECT® Protein Kinase C (PKC) Assay System to measure activity of protein kinase C in soluble (cytosolic) and particulate (membrane) fractions of Raji B cells following stimulation with the phorbol ester TPA. (0424)

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J. Neurosci. 18, 5285-5293. Vasoactive Intestinal Peptide Enhances its own Expression in Sympathetic Neurons After Injury 1998

Mohney, R.P. and Zigmond, R.E.

Notes: The SignaTECT® cAMP-Dependent Protein Kinase (PKA) Assay System was used in this study. (2190)

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Development 125, 2291-2302. YakA, a protein kinase required for the transition from growth to development in Dictyostelium 1998

Souza, G.M., Lu, S., Kuspa, A.

Notes: The SignaTECT® cAMP-dependent Protein Kinase Assay System was used to measure PKA activity in Dictyostelium. Ten micrograms of cell protein was assayed in the presence of the absence of a PKA inhibitor. (0358)

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J. Biol. Chem. 272, 20063-20069. Adrenocorticotropin Induction of Stress-activated Protein Kinase in the Adrenal Cortex in vivo 1997

Watanabe, G., Pena, P., Albanese, C., Wilsbacher, L.D., Young, J.B. and Pestell, R.G.

Notes: The SignaTECT® Protein Kinase C (PKC) Assay System was used in this study. (2191)

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J. Biol. Chem. 272, 30356-30361.. Antioxidant-induced Nuclear Translocation of CCAAT/Enhancer-binding Protein beta. A critical role for protein kinase a-mediated phosphorylation of ser299 1997

Chinery, R. , Brockman, J. A. , Dransfield, D. T. , Coffey, R. J.

Notes: Kinase assays were performed on DKO-1 colon carcinoma cells following treatment with the antioxidant PDTC or forskolin for up to 24hr . Assays were performed using the SignaTECT® cAMP-dependent Protein Kinase Assay System (PKA). Details on how to perform the simple cell extraction procedure are provided. (1340)

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J. Biol. Chem. 272, 17929-17936. Apoptosis Induced by Gliotoxin Is Preceded by Phosphorylation of Histone H3 and Enhanced Sensitivity of Chromatin to Nuclease Digestion 1997

Waring, P., Khan, T., Sjaarda, A.

Notes: Thymocyte cells extracts were assayed for PKA activity using SignaTECT® cAMP-Dependent Protein Kinase (PKA) Assay System under a variety of pretreatments . (0209)

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Am. J. Physiol. 273, C1981-C1986. Regulation of rat Na(+)-K(+)-ATPase activity by PKC is modulated by state of phosphorylation of Ser-943 by PKA. 1997

Cheng, X. J. , Hoog, J. O. , Nairn, A. C. , Greengard, P. , Aperia, A.

Notes: This paper describes use of the SignaTECT® cAMP-Dependent Protein Kinase Assay System to study activation of rat Na(+)-K(+)-ATPase by PKA and PKC in COS cells. (1336)

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J. Clin. Invest. 100, 1180-1192. Stimulus coupling to transcription versus secretion in pheochromocytoma cells: Convergent and divergent signal transduction pathways and the crucial roles for route of cytosolic calcium entry and protein kinase C. 1997

Tang, K., Wu, Mahata, S.K., Mahata, M., Gill, B.M., Parmer, R.J. and O’Conner, D.T.

Notes: Kinase assays were performed on PC12 cell extracts. A lot of detail is given on preparation of cell extracts. Kinase activities were measured in the cytosol and membrane fractions. Kinase activities were determined in the presence or absence of 1mM nicotine. The SignaTECT® Protein Kinase C (PKC) and cAMP-Dependent (PKA) Protein Kinase Assay Systems were used in this study. (1532)

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