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J. Biol. Chem. 272, 18564-18571. Mutational analysis of the murine granzyme B gene promoter in primary T cells and a T cell clone. 1997

Babichuk, C.K. and Bleackley, R.C.

Notes: The pGL2 Basic Vector and the pSV β-Galactosidase Control Vector were used in conjunction with the Luciferase Assay System to study gene regulation in primary mouse lymphocytes and the murine CTL line MTL 2.8.2. (1487)

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J. Neurosci. 17, 6122-6132. Nerve growth factor induces transcription of the p21 WAF1/CIP1 and cyclin D1 genes in PC12 cells by activating the Sp1 transcription factor. 1997

Yan, G. Z. , Ziff, E. B.

Notes: Expression of luciferase reporter constructs was studied in PC12 cells using constructs prepared in the pGL2 Basic Vector. (0136)

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J. Biol. Chem. 272, 5936-5942. Nerve growth factor up-regulates the N-methyl-D-aspartate receptor subunit 1 promoter in PC12 cells. 1997

Bai, G. and Kusiak, J.W.

Notes: Reporter vectors (produced with the pGL2 Basic Vector) were transiently transfected into PC12 cells and promoter activity was measured in response to 2.5S NGF. Promoter constructs were generated by PCR, subcloned into the pGEM®-T Vector and sequenced. The pGEM®-luc Vector was used to generated an antisense luc RNA probe used in RNase protection assays (using RNase ONE™ Ribonuclease) to follow the transcription of luciferase DNA following NGF treatment. The early growth reaction transcription factor and variants were expressed in the TNT® SP6 Coupled Reticulocyte Lysate System and used to perform gel shifts with promoter elements. The SP1 Consensus Oligonucleotide was used to compete transcription factor binding to the promoter elements. (1493)

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EMBO J. 16, 4276-4284.. Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence. 1997

Dobbelstein, M. , Roth, J. , Kimberly, W. T. , Levine, A. J. , Shenk, T.

Notes: CAT reporter activity was normalized to luciferase activity using the Luciferase Assay System. Luciferase was provided by the pGL3 Control Vector and CAT activity was measured using the CAT Enzyme Assay System. (1237)

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J. Biol. Chem. 272, 2396-2403.. Oct-1 and CCAAT/enhancer-binding protein (C/EBP) bind to overlapping elements within the interleukin-8 promoter. The role of Oct-1 as a transcriptional repressor. 1997

Wu, G. D. , Lai, E. J. , Huang, N. , Wen, X.

Notes: Expression of luciferase reporter constructs, derived from the pGL2-Basic Vector, was studied in Caco-2 and HepG2 cells. (0155)

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J. Biol. Chem. 272, 7968-7976. Organization and regulatory aspects of the human intestinal mucin gene (MUC2) locus. 1997

Velcich, A. , Palumbo, L. , Selleri, L. , Evans, G. , Augenlicht, L.

Notes: Expression of luciferase reporter genes, derived from pGL2 Vectors, was performed in HT29 and LS174T human intestinal cell line and HeLa cells. The Tfx-50™ Reagent was used to transfect the intestinal cell lines. (0220)

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J. Biol. Chem. 272, 26620-26626. Regulation of clusterin gene expression by transforming growth factor β. 1997

Jin, G., Howe, P.H.

Notes: Luciferase reporter studies performed in CCL64 (Mv1Lu) mink lung cells using constructs prepared in the pGL2-Basic Vector and were normalized to β-galactosidase activity. Studies performed in 10T1/2, 3Tp, HeLa cells and primary bovine aortic endothelial cells were normalized to the Renilla luciferase. A five to one (w/w) ratio of experimental to control vector was used with pRL-CMV Control Vector. A two to one (w/w) ratio of experimental to control vector was used with pSV-β-Galactosidase Control Vector. (0981)

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J. Biol. Chem. 272, 14954-14960.. Regulation of human apolipoprotein A-I gene expression by gramoxone. 1997

Cuthbert, C. , Wang, Z. , Zhang, X. , Tam, S. P.

Notes: Luciferase studies were performed in HepG2 cells using constructs prepared in pGL2 Basic or pGL2 Promoter Vectors. (1292)

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Blood 89, 2394-2403. Regulation of plasminogen gene expression by interleukin-6. 1997

Jenkins, G.R., Seiffert, D., Parmer, R.J., Miles, L.A.

Notes: Luciferase reporter studies were performed in Hep3B cells, and constructs were prepared in the pGL2-Basic and -Promoter Vectors. Luciferase activity was measured with the Luciferase Assay System. (0974)

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J. Mol. Neurosci. 8, 223-241. Regulation of the neural-specific gene VGF in PC12 cells: Identification of transcription factors interacting with NGF-responsive elements. 1997

Luc, P.V. and Wagner, J.A.

Notes: Reporter assays were performed in PC12 cells (1585)

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J. Clin. Invest. 100, 972-985.. Role of NFκB in the mortality of sepsis 1997

Bohrer, H., Qiu, F., Zimmermann, T., Zhang, Y., Jllmer, T., Mannel, D., Bottiger, B.W., Stern, D.M., Waldherr, R., Saeger, H.D., Ziegler, R., Bierhaus, A., Martin, E. and Nawroth, P.P.

Notes: Intravenous somatic gene transfer with an expression plasmid coding for IκBα was used to investigate the role of members of the NFκB family in a mouse model of endotoxemia. Mouse kidneys were homogenized in 1X Cell Culture Lysis Reagent (CCLR) and assayed with the Luciferase Assay System. Reporter constructs were prepared in the pGL2 Basic and pGL2 Control Vectors. Please note the Anti-Luciferase pAb reported in the paper is different than the one currently sold by Promega. (1423)

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Proc. Natl. Acad. Sci. USA 94(2), 422-427. rRNA-like sequences occur in diverse primary transcripts: Implication for the control of gene expression. 1997

Mauro, V.P. and Edelman, G.M.

Notes: These studies were performed in C6 glioma cells. (2249)

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J. Biol. Chem. 272, 14263-14271. Steroidogenic factor-1 is an essential transcriptional activator for gonad-specific expression of promoter I of the rat prolactin receptor gene. 1997

Hu, Z., Zhuang, L., Guan, X., Meng, J., Dufau, M.L.

Notes: Luciferase studies were performed in MLTC-1 mouse Leydig tumor cells and HepG2 cells. Experimental constructs were prepared in the pGL2-Basic Vector. (1026)

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J. Biol. Chem. 272, 7501-7505. Structure and expression of H-type GDP-L-fucose:β-D-galactoside 2-α-L-fucosyltransferase gene (FUT1). Two transcription start sites and alternative splicing generate several forms of FUT1 mRNA. 1997

Koda, Y., Soejima, M., Kimura, H.

Notes: Luciferase studies were performed in MCAS ovarian cancer cells. Luciferase constructs were prepared in the pGL2-Enhancer Vector. (0915)

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J. Biol. Chem. 272, 14899-14907. Synergy between interferon-γ and tumor necrosis factor-α in transcriptional activation is mediated by cooperation between signal transducer and activator of transcription 1 and nuclear factor kappaB. 1997

Ohmori, Y. , Schreiber, R. D. , Hamilton, T. A.

Notes: Luciferase studies were performed in STAT1-deficient mouse fibroblasts and NIH3T3 cells using constructs prepared in the pGL2 Basic Vector. The luciferase activities were determined with the Luciferase Assay System. (0593)

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EMBO J. 16, 4007-4020. T-cell subset-specific expression of the IL-4 gene is regulated by a silencer element and STAT6. 1997

Kubo, M., Ransom, J., Webb, D., Hashimoto, Y., Tada, T., Nakayama, T.

Notes: Luciferase reporter studies were performed in BW5147 thymomas, EL-4 thymomas, 68-41 T-cell hybridomas, MS-SB cloned T-cells, P3U1 B-cells, L929 fibroblasts, PAM212 keratinocytes, and 1308-1 thymic epithelial cells using constructs prepared in the pGL2-Basic Vector. (0854)

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J. Neurosci. 17, 4149-4158. The characterization of the Olf-1/EBF-like HLH transcription factor family: Implications in olfactory gene regulation and neuronal development. 1997

Wang, S.S., Tsai, R.Y.L. and Reed, R.R.

Notes: The authors used Promega's pGL2 Basic Vector, pGL2 Promoter Vector and Luciferase Assay System. Studies on the Olf-1/EBF-like HLH transcription factor family were performed in human embryonic kidney 293 cells. (1548)

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J. Biol. Chem. 272, 1188-1196. The mitogen-activated protein kinase and JAK-STAT signaling pathways are required for an oncostatin M-responsive element-mediated activation of matrix metalloproteinase 1 gene expression. 1997

Korzus, E., Nagase, H., Rydell, R., Travis, J.

Notes: Luciferase studies were performed in normal human dermal fibroblasts and primary human astrocytes. The experimental constructs were prepared in the pGL2-Basic and pGL2-Promoter Vectors. All luciferase activities (determined with the Luciferase Assay System) were normalized to β-Galactosidase activity. (0884)

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J. Biol. Chem. 272, 13426-13431. The polymyositis-scleroderma autoantigen interacts with the helix-loop-helix proteins E12 and E47. 1997

Kho, C.J., Huggins, G.S., Endege, W.O., Patterson, C., Jain, M.K., Lee, M.E., Haber, E.

Notes: Luciferase reporter studies were performed in NIH3T3 cells. The researchers created sort of a two-hybrid pGL2 Vector to demonstrate that two cotransfected proteins can interact and bind an enhancer element, thus increasing luc activity. Researchers expressed one protein as a GST fusion and the other as a 35S-met protein in the TNT® Coupled Reticulocyte Lysate System and showed that the 35S-protein could be pulled out of the lysate. (0937)

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J. Biol. Chem. 272, 5579-5586. The promoters for human and monkey poliovirus receptors. Requirements for basic and cell type-specific activity. 1997

Solecki, D., Schwarz, S., Wimmer, E., Lipp, M., Bernhardt, G.

Notes: Luciferase studies were performed in L929, COS-1, HeLa, HEp2 and Raji Cells. Experimental constructs were prepared in the pGL2 Basic Vector and extracts were made with Reporter Lysis Buffer then assayed with the Luciferase Assay System. (0348)

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J. Biol. Chem. 272, 20522-20530. The rat pyruvate carboxylase gene structure. Alternate promoters generate multiple transcripts with the 5´-end heterogeneity. 1997

Jitrapakdee, S., Booker, G.W., Cassady, A.I., Wallace, J.C.

Notes: Luciferase reporter studies were performed in COS-1 cells using constructs prepared in the pGL3-Basic Vector. DNA sequencing was accomplished with the fmol® DNA Cycle Sequencing System. (0983)

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Genomics 46, 459-65. The structural organization of the human Na+/myo-inositol cotransporter (SLC5A3) gene and characterization of the promoter. 1997

Mallee, J.J., Atta, M.G., Lorica, V., Rim, J.S., Kwon, H.M., Lucente, A.D., Wang, Y., Berry, G.T.

Notes: Cloned promoter from human SLC5A3 gene upstream of firefly luciferase in pGL2 Basic Vector. As a positive control, the beta actin promoter was cloned into pGL2 Basic Vector. Co-transfected SLC5A3 promoter constructs with the beta actin construct and pRL-CMV Vector at a ratio of 1:40:1. Measured luciferase activities with the Dual-Luciferase® Reporter Assay System. (0726)

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J. Cell Biol. 138, 1343-1354. Tissue-specific expression of the L1 cell adhesion molecule is modulated by the neural restrictive silencer element. 1997

Kallunki, P., Edelman, G.M. and Jones, F.S.

Notes: Studies were performed in NIH 3T3 and Neuro2a cells. The pGEM®-luc Vector was used as a source of the luciferase gene to convert previous beta-Galactosidase reporter vector constructs to luciferase reporter vectors. The TnT® Coupled Reticulocyte Lysate System was used to verify that the cloned NRSF/REST protein could be expressed intact. (1566)

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J. Biol. Chem. 272, 25890-25898. Transcription of human ABO histo-blood group genes is dependent upon binding of transcription factor CBF/NF-Y to minisatellite sequence. 1997

Kominato, Y., Tsuchiya, T., Hata, N., Takizawa, H., Yamamoto, F.I.

Notes: Luciferase studies were performed in KATO III human gastric cancer cells using constructs prepared in the pGL3-Basic and pGL3-Promoter Vectors. (0879)

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J. Biol. Chem. 272, 19575-19581. Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. 1997

Buckhaults, P., Chen, L., Fregien, N., Pierce, M.

Notes: Luciferase studies were performed in HepG2 cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL2-Basic Vector. Transfection efficiency was standardized using the β-Galactosidase Assay System with Reporter Lysis Buffer. (2245)

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