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J. Biol. Chem. 272, 23659-23667. Differential transcriptional regulation of the two vascular endothelial growth factor receptor genes. 1997

Gerber, H-P., Condorelli F., Park J. and Ferrara N.

Notes: Promega's pGL2 Vectors; Dual-Luciferase® Reporter Assay System and Access RT-PCR System were used in this study. (1992)

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J. Biol. Chem. 272, 30866-30872. Dimerization Properties of Human BAD. Identification of a bh-3 domain and analysis of its binding to mutant bcl-2 and bcl-xl proteins 1997

Ottilie, S., Diaz, J.L., Horne, W., Chang, J., Wang, Y., Wilson, G., Chang, S., Weeks, S., Fritz, L.C., Oltersdorf, T.

Notes: The pCI-neo Mammalian Expression Vector was used to express the 168 amino acid BAD protein in 293 cells. Cells expressing the BAD protein were used for apoptosis studies. (0573)

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Mol. Pharmacol. 51, 963-971. DNA elements recognizing NF-Y and Sp1 regulate the human multidrug- resistance gene promoter. 1997

Sundseth, R., MacDonald, G., Ting, J., King, A. C.

Notes: Luciferase reporter studies were performed in HepG2 and HTC116 cells using constructs prepared in the pGL2 Basic Vector. Reporter activity was measured with the Luciferase Assay System. (0320)

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J. Biol. Chem. 272, 22259-22264. Down-regulation of the cyclin A promoter by transforming growth factor- beta1 is associated with a reduction in phosphorylated activating transcription factor-1 and cyclic AMP-responsive element-binding protein 1997

Yoshizumi, M. , Wang, H. , Hsieh, C. M. , Sibinga, N. E. S. , Perrella, M. A. , Lee, M. E.

Notes: Expression of luciferase reporter constructs derived from the  pGL2 Basic Vector were studied in Mv1Lu mink lung cells. Luciferase activity was normalized to control β-Galactosidase activity. (0117)

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J. Biol. Chem. 272, 16540-16547. Epidermal growth factor and okadaic acid stimulate Sp1 proteolysis. 1997

Mortensen, E.R., Marks, P.A., Shiotani, A., Merchant, J.L.

Notes: Used the Luciferase Assay System and the CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS) in their studies on GH4 rat pituitary adenoma cells. Reporter constructs were prepared in the pGL2 Basic Vector. (0667)

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Proc. Natl. Acad. Sci. USA 94, 12309-12313. Expression of hepatocyte nuclear factor 6 in rat liver is sex-dependent and regulated by growth hormone. 1997

Lahuna, O., Fernandez, L., Karlsson, H., Maiter, D., Lemaigre, F.P., Rousseau, G.G., Gustafsson, J., Mode, A.

Notes: Dual-Luciferase® Reporter Assay System studies were performed in HepG2 cells. The experimental plasmid constructed in pGL2-Basic Vector was used at a 40:1 ratio over the pRL-CMV Vector. The experimental and control plasmids were cotransfected with a third plasmid expressing hepatocyte nuclear factor 6 from a CMV promoter. The luciferase activity expressed in the presence or absence of the hepatocyte factor were measured. The TNT® Coupled Wheat Germ Extract System was used to in vitro translate four transcription factors for assay of gel shift with the promoter construct. (0870)

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J. Biol. Chem. 272, 5854-5860. Expression of the rat m4 muscarinic acetylcholine receptor gene is regulated by the neuron-restrictive silencer element/repressor element 1. 1997

Mieda, M., Haga, T., Saffen, D.W.

Notes: Luciferase studies were performed in the rat/mouse cell line NG108-15 (a hybrid of the mouse neuroblastoma N18 and rat glioma C6). Experimental constructs were prepared in the pGL2 Basic Vector. (0690)

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J. Biol. Chem. 272, 18250-18260.. Functional Interactions between the Estrogen Receptor and the Transcription Activator Sp1 Regulate the Estrogen-dependent Transcriptional Activity of the Vitellogenin A1 io Promoter 1997

Batistuzzo de Medeiros, S. R. , Krey, G. , Hihi, A. K. , Wahli, W.

Notes: Luciferase studies were performed in Drosophila SL-2 cells using constructs prepared in the pGL2 Basic Vector. The purified recombinant SP1 protein was used for both DNase I footprinting (1261)

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Proc. Natl. Acad. Sci. USA 94, 7549-7554. Gestational exposure to ethanol suppresses msx2 expression in developing mouse embryos 1997

Rifas, L., Towler, D.A., Avioli, L.V.

Notes: Luciferase studies were performed on transfected MC3T3-E1 cell lysates using the Luciferase Assay System. Constructs were prepared in the pGL2 Promoter Vector. (0476)

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J. Biol. Chem. 272, 22905-22912. Glomerular Mesangial Cell-specific Transactivation of Matrix Metalloproteinase 2 Transcription Is Mediated by YB-1 1997

Mertens, P.R., Harendza, S., Pollock, A.S., Lovett, D.H.

Notes: Luciferase studies were performed in mesanglia cells and glomerular epithelial cells. The experimental constructs were made in the pGL2 Basic and pGL2 Promoter Vectors. Luciferase activity was normalized to β-Galactosidase activity via cotransfection with the pSV-β-Galactosidase Control Vector. (0683)

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J. Biol. Chem. 272, 22776-22780. Glucose Catabolism in Cancer Cells. The type ii hexokinase promoter contains functionally active response elements for the tumor suppressor p53. 1997

Mathupala, S.P., Heese, C., Pedersen, P.L.

Notes: Luciferase studies were performed in the AS-30D hepatoma cell line using constructs made in the pGL2 Basic Vector. The effect of p53 on the hexokinase promoter was assayed in vivo by expression of the p53 from the pCI-neo Mammalian Expression Vector and promoter activity was reported with luciferase. All transfections were normalized to β-Galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. (0700)

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Proc. Natl. Acad. Sci. USA 94, 3837-3841. Gypsy retrotransposon as a tool for the in vivo analysis of the regulatory region of the optomotor-blind gene in Drosophila. 1997

Tsai, S.F., Jang, C.C., Prikhod'ko, G.G., Bessarab, D.A., Tang, C.Y., Pflugfelder, G.O , Sun, Y.H.

Notes: The authors cloned a 5.7 kb PCR product into pGEM®-T Vector (0237)

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Gene Ther. 4, 162-171. High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid-DNA complex. 1997

Keogh, M.C., Chen, D., Lupu, F., Shaper, N., Schmitt, J.F., Kakkar, V.V., Lemoine, N.R.

Notes: The authors used Tfx™-50 Reagent to transfect or microinject pGL3-Basic Vector constructs into rabbit, rat and human arterial smooth muscle cells and HepG2 cell lines.  For the rabbit carotid arteries, this was an in vivo transfer of DNA. (0930)

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J. Biol. Chem. 272, 8013-8018. Hyaluronan fragments induce nitric-oxide synthase in murine macrophages through a nuclear factor kappaB-dependent mechanism. 1997

McKee, C.M., Lowenstein, C.J., Horton, M.R., Wu, J., Bao, C., Chin, B.Y., Choi, A.M., Noble, P.W.

Notes: Reporter studies were performed in the mouse macrophage-like cell line RAW 264.7. Promoter constructs were prepared in the pGL2 Basic Vector and luciferase activity monitored with the Luciferase Assay System. Transfections were normalized by co-transfecting the pSV-β-Galactosidase Control Vector. The β-gal activity was assayed with the β-Galactosidase Assay System. (0713)

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J. Neurosci. 17, 7583-7593. Identification of a novel repressive element that contributes to neuron-specific gene expression. 1997

Weber, J.R.M. and Skene, J.H.P.

Notes: Reporter studies were performed in primary cultures of dissociated rat cerebral cortex and HTC hepatoma cells. The ratio of activity in neuronal cells to hepatoma cells was used as a measure of promoter specificity. Promega's pGL2 Basic Vector and Luciferase Assay System were used in these studies. (1549)

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J. Virol. 71, 1651-1656. Identification of a replication-competent pathogenic human immunodeficiency virus type 1 with a duplication in the TCF-1alpha region but lacking NF-kappaB binding sites. 1997

Zhang, L. , Huang, Y. , Yuan, H. , Chen, B. K. , Ip, J. , Ho, D. D.

Notes: THe pGL2-Basic Vector was used to create reporter constructs for luciferase expression studies. (0094)

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J. Biol. Chem. 272, 2722-2728. Identification of a retinoid/chicken ovalbumin upstream promoter transcription factor response element in the human retinoid X receptor gamma2 gene promoter. 1997

Barger, P.M. and Kelly, D.P.

Notes: The Luciferase Assay System and the pGL2 Basic Vector were used to study gene expression in CV-1 cells. (1460)

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J. Biol. Chem. 272, 10367-10371. Identification of an element required for acetylcholine receptor- inducing activity (ARIA)-induced expression of the acetylcholine receptor epsilon subunit gene. 1997

Si, J., Miller, D. S., Mei, L.

Notes: Luciferase studies were performed in C2C12 myoblasts. Experimental constructs were prepared in the pGL2 Basic Vector, and luciferase activities were determined with the Luciferase Assay System. (0403)

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J. Biol. Chem. 272, 16453-16465. Identification of transcription factor binding sites important in the regulation of the human interleukin-5 gene 1997

Stranick, K. S., Zambas, D. N., Uss, A. S., Egan, R. W. Billah, M. M., Umland, S. P.

Notes: Luciferase studies were performed in Th2 cells and D10.G4.1 T cells using constructs prepared in the pGL2 Basic Vector. (0341)

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Am. J. Physiol. 273, L1090-L1095. Induction of aquaporin 3 by corticosteroid in a human airway epithelial cell line. 1997

Tanaka, M., Inase, N., Fushimi, K., Ishibashi, K., Ichioka, M., Sasaki, S. and Marumo, F.

Notes: A 318bp promoter region from genomic DNA was amplified by PCR and ligated into the pGEM®-T Vector. This promoter region was then cloned into the pGL2-Basic Vector, and activity was measured using the Luciferase Assay System. Transfection efficiency was monitored with the β-Galactosidase Enzyme Assay System. (0300)

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J. Biol. Chem. 272, 791-797. Inhibition of myogenesis by multiple cyclin-Cdk complexes. Coordinate regulation of myogenesis and cell cycle activity at the level of E2F. 1997

Guo, K., Walsh, K.

Notes: Perform luciferase studies in C2C12 myocytes and 10T1/2 cells using constructs prepared in the pGL2-Basic Vector. Luciferase activity was normalized to soluble alkaline phosphatase control activity. (1087)

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J. Clin. Invest. 100, 1797-1803. Interleukin 6 is autoregulated by transcriptional mechanisms in cultures of rat osteoblastic cells. 1997

Franchimont, N. , Rydziel, S. , Canalis, E.

Notes: Luciferase studies were performed in primary rat osteoblasts using both the pGL2 Basic and pGL3 Basic vectors. (1168)

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J. Biol. Chem. 272, 17795-17801. Lipopolysaccharide Induction of the Tumor Necrosis Factor-alpha Promoter in Human Monocytic Cells. Regulation by egr-1, c-jun, and nf- kappab transcription factors 1997

Yao, J. , Mackman, N. , Edgington, T. S. , Fan, S. T.

Notes: Expression of luciferase reporter constructs, derived from the pGL2 Promoter Vector, was studied in THP-1 monocytes. (0104)

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J. Biol. Chem. 272, 13683-13689. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. 1997

Fang, X. , Gibson, S. , Flowers, M. , Furui, T. , Bast, R. C. Jr, Mills, G. B.

Notes: Luciferase assays were performed in Swiss 3T3 cells. Researchers replaced CAT in their old vectors with the luc gene from pGL2 Basic. (1193)

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J. Biol. Chem. 272, 20502-20507. Murine laminin alpha3A and alpha3B isoform chains are generated by usage of two promoters and alternative splicing 1997

Ferrigno, O. , Virolle, T. , Galliano, M. F. , Chauvin, N. , Ortonne, J. P. , Meneguzzi, G. , Aberdam, D.

Notes: Authors performed luciferase studies in PAM212 mouse keratinocytes and NIH3T3 cells using constructs prepared in the pGL2 Basic and pGL2 Promoter Vectors. (1152)

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