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Genome Res. 10, 959-966. Reverse transcriptase-polymerase chain reaction validation of 25 'Orphan' genes from Escherichia coli K-12 MG1655 2000

Alimi, J.P., Poirot, O., Lopez, F. and Claverie, J. M.

Notes: The Access RT-PCR System was used to assess whether some uncharacterized E. coli sequences that have putative open reading frames are in fact transcribed. The RT-PCR samples contained 0.1µg of bacterial total RNA, and were amplified for 35 cycles. (2158)

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Genetics 154, 1115-1123. Three subfamilies of pheromone and receptor genes generate multiple B mating specificities in the mushroom Coprinus cinereus. 2000

Halsalla, J., Milnera, M., Casseltona, L.

Notes: Poly(A)+ RNA was purified from total RNA by the PolyATtract® mRNA Isolation System. PCR products were purified by agarose gel electrophoresis and were cloned into pGEM®-T and pGEM®-T Easy Vector. Genes were obtained by RT-PCR using the Access RT-PCR System. (1097)

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Infect. Immun. 68, 3368–3376. Transriptional Organization and Function of Invasion Genes within Salmonella enterica Serovar Typhimurium Pathogenicity Island 1, Including the prgH, prgI, prgJ, prgK, orgA, orgB, and orgC Genes 2000

Klein, J.R., Fahlen, T.F., and Jones, B.D.

Notes: Total RNA was isolated from a virulent strain of Salmonella enterica, SL1344 using the SV Total RNA Isolation System. The Access RT-PCR System was used to characterize the transcriptional organization of the prg operon. The pgrH gene was amplified by RT-PCR and cloned into the pGEM®-T vector (2305)

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J. Gen. Virol. 80, 1231-1240. A highly pathogenic simian/human immunodeficiency virus with genetic changes in cynomolgus monkey 1999

Shinohara, K., sakai, K., ando, S., Ami, Y., Yoshino, N., Takahashi, E., Someya, K., Suzaki, Y., Makasone, T., Sasaki, Y., Kaizu, M., Lu, Y., Honda, M.

Notes: Poly A+ RNA was isolated from infected cynomolgus monkey plasma and used for RT-PCR with the Access RT-PCR System. (0396)

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J. Immunol. 162, 871-877. Alternative splicing and hypermutation of a nonproductively rearranged TCR alpha-chain in a T cell hybridoma. 1999

Marshall, B., Schulz, R., Zhou, M., Mellor, A.

Notes: The Access RT-PCR System was used to amplify Vα1024 mRNA of the TCR alpha chain and the resulting product was cloned with the pGEM®-T Vector System. (0734)

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J. Virol. 73, 8160-8166. Bovine leukemia virus structural gene vectors are immunogenic and lack pathogenicity in a rabbit model. 1999

Kucerova, L., Altaverova, V., Altaner C., Boris-Lawrie, K.

Notes: The RNAgents® Total RNA Isolation System was used to isolate RNA from LPS-stimulated, bovine leukemia virus-infected peripheral blood mononuclear cells. The RNA was treated with DNase and used for RT-PCR with the Access RT-PCR System. One fiftieth of the reaction was used for nested PCR. (0855)

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J. Biol. Chem. 274, 35845-35854.. Brain actin-associated protein phosphatase 1 holoenzymes containing spinophilin, neurabin, and selected catalytic subunit isoforms. 1999

MacMillan, L.B., Bass, M.A., Cheng, N., Howard, E.F., Tamura, M., Strack, S., Wadzinski, B.E., Colbran, R.J.

Notes: The Access RT-PCR System was used to amplify messages for spinophilin and neurabin from rat brain total RNA. The products were used to make probes to screen a cDNA library. (0720)

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J. Biol. Chem. 274, 29510-29518. Characterization of the Shank family of synaptic proteins: Multiple genes, alternative splicing and differential expression in brain and development. 1999

Lim, S., Naisbitt, S., Yoon, J., Hwang, J.I., Suh, P.G., Sheng, M., Kim, E.

Notes: Total RNA was isolated from the cortex and cerebellum of adult and developing rat brains with the RNAgents® Total RNA Isolation System. RT-PCR was performed on a variety of samples with a variety of primers to assay alternative splicing. The Access RT-PCR System was used for all RT-PCR. (0764)

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J. Immunol. 162, 1232-1235. Cutting edge: Coordinate regulation of IFN regulatory factor-1 and the polymeric Ig receptor by proinflammatory cytokines. 1999

Blanch, V.J., Piskurich, J.F., Kaetzel, C.S.

Notes: The authors used the Access RT-PCR System to perform quantitative RT-PCR from total RNA. (2239)

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J. Biol. Chem. 274, 17257-17266.. Divalent cations differentially regulate integrin alpha II b cyotplasmic tail binding to beta 3 and to calcium- and integrin-binding protein 1999

Vallar, L., Melchior, C., Plançon, S., Drobecq, H., Lippens, G., Regnault, V., Kieffer, N.

Notes: Total RNA was extracted from HEL-5J20 and used to generate a full length cDNA (576bp) of the calcium- and intergrin-binding protein using the Access RT-PCR System. The subsequent product was purified with the Wizard® PCR Preps System, digested with restriction enzymes and cloned into a GST-fusion expression vector. (0214)

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J. Invest. Dermatol. 113, 43–48. Enhanced expression of eotaxin and CCR3 in atopic dermatitis. 1999

Yawalker, N., Uguccioni, M., Scharer, J., Braunwalder, J., Karlen, S., Dewald, B., Braathen, L.R. and Baggiolini, M.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

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Science 285, 248–251. HMG-1 as a late mediator of endotixin lethality in mice. 1999

Wang, H., Bloom, O., Zhang, M., Vishnubhakat, J.M., Ombrellino, M., Che, J., Frazier, A., Yang, H., Ivanova, S., Borovikova, L., Manogue, K.R., Faist, E., Abraham, E., Andersson, J., Andersson, U., Molina, P.E., Abumrad, N.N., Sama, A. and Tracey, K.J.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

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Mol. Cell 4, 1-10. Mitotic checkpoint inactivation fosters transformation in cells lacking the breast cancer susceptibility gene, Brca2. 1999

Lee, H., Trainer, A.H., Friedman, L.S., Thistlethwaite, F.C., Evans, M.J., Ponder, B.A.J., Venkitaraman, A.R.

Notes: The p53 cDNAs expressed from thymic lymphoma cells were amplified with the Access RT-PCR System and the resulting products were cloned with the pGEM®-T Vector System. No details of the reaction or whether or not total RNA was used were provided. (0812)

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Genetics 152, 519-528. MOD-D, a galpha subunit of the fungus podospora anserina, is involved in both regulation of development and vegetative incompatibility. 1999

Loubradou, G. , Begueret, J. , Turcq, B.

Notes: Synthesis of the mod-D1 cDNA and PCR amplification was performed using the Access RT-PCR System. (0744)

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J. Bacteriol. 181, 3310–3316. NahY, a Catabolic Plasmid-Encoded Receptor Required for Chemotaxis of Pseudomonas putida to the Aromatic Hydrocarbon Naphthalene. 1999

Grimm, A.C. and Harwood, C.S.

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

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J. Immunol. 162, 5511-5518. Neutralization of the CXC chemokine, macrophage inflammatory protein-2, attentuates bleomycin-induced pulmonary fibrosis. 1999

Keane, M.P., Belperio, J.A., Moore, T.A., Moore, B.B., Arenberg, D.A., Smith, R.E., Burdick, M.D., Kunkel, S.L., Strieter, R.M.

Notes: The Access RT-PCR System was used to analyze collagen gene expression of primary fibroblasts treated with various agents. The messages were amplified from 100, 200 and 400ng of total RNA. (0928)

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J. Invest. Dermatol. 113, 711–719. Regulation of the melanoma cell adhesion molecule gene in melanoma: Modulation of mRNA synthesis by cyclic adenosine monophosphate, phorbol ester, and stem cell factor/c-Kit signaling. 1999

Karlen, S. and Braathen, L.R.

Notes: The SV Total RNA Isolation System was used to extract total RNA from 106 SK-Mel2 human melanoma cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. The results were used for semi-quantitative analysis. (0959)

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J. Biol. Chem. 274, 26850-26859. Requirement of an E1A-sensitive coactivator for long-range transactivation by the beta-globin locus control region. 1999

Forsberg, E.C., Johnson, K., Zaboikina, T.N., Mosser, E.A., Bresnick, E.H.

Notes: The Access RT-PCR System was used to amplify gamma-globulin sequences and HPRT from total RNA equivalent to 16, 667, and 3333 cells. The pGL2 Basic Vector was used to construct a GAL reporter vector for two-hybrid analysis. (1165)

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Brain Res. 838, 166-170. Single-cell RT-PCR demonstrates expression of voltage-dependent chloride channels (C1C-1, C1C-2 and C1C-3) in outer hair cells of rat cochlea. 1999

Kawasaki, E., Hattori, N., Miyamoto, E., Yamashita, T., Inagaki, C.

Notes: The initial work was performed with a single-tube RT-PCR system from a company in which both the reverse transcriptase and polymerase were mixed together and thus could not be separated. Consequently, the authors could not produce the important no-reverse transcriptase control. To do such a control, the authors used the Access RT-PCR System, which allows such control, since both the Tfl DNA Polymerase and AMV Reverse Transcriptase are added separately to the reaction. (0925)

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J. Biol. Chem. 274, 32145-32152. SPARC regulates the expression of collagen type I and transforming growth factor-beta1 in mesangial cells. 1999

Francki, A., Bradshaw, A.D., Bassuk, J.A., Howe, C.C., Couser, W.G., Sage, E.H.

Notes: The Access RT-PCR System was used for analytical RT-PCR for a variety of targets. Amplification was performed within the linear range of PCR product formation and normalized to the beta-tubulin signal or rpS6 signal. (1169)

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Am. J. Hum. Genet. 65, 1547–1560. The molecular basis of Sjogren-Larsson syndrome: Mutation analysis of the fatty aldehyde dehydrogenase gene. 1999

Rizzo, W. B., Carney, G. and Lin, Z.

Notes: Genomic DNA was purified from blood or cultured fibroblasts, by means of the Wizard® Genomic DNA Purification Kit. Total RNA was isolated from cultured fibroblasts by use of the SV Total RNA Isolation System, and it was amplified by Access RT-PCR System by use of exonic primers. An expression vector containing an S-tag attached to the 5´ end of the FALDH cDNA was constructed using pCI-neo Mammalian Expression Vector. (0482)

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Infect. Immun. 66, 1534-1537. Phlebotomus papatasi saliva inhibits protein phosphatase activity and nitric oxide production by murine macrophages 1998

Waitumbi, J., Warburg, A.

Notes: The Access RT-PCR System was used to determine HPRT (hypoxanthine-guanine phosphoribosyltransferase) and iNOS (induced nitric oxide synthase) mRNA in salivary gland lysate of P. papatasi. The Griess Reagent System was used to measure the nitrite accumulation in macrophage culture media. (0191)

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Genetics 148, 1081-10. A large pheromone and receptor gene complex determines multiple B mating type specificities in Coprinus cinereus. 1998

O'Shea, S.F., Chaure, P.T., Halsall, J.R., Olesnicky, N.S., Leibbrandt, A., Connerton, I.F , Casselton, L.A.

Notes: cDNAs for the receptor genes were obtained by RT-PCR using the Access RT-PCR System. (0569)

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J. Mol. Neurosci. 10, 181-192. Amyloid beta-peptide (1-40)-mediated oxidative stress in cultured hippocampal neurons. Protein carbonyl formation, CK BB expression, and the level of Cu, Zn, and Mn SOD mRNA. 1998

Aksenov, M.Y., Aksenova, M.V., Markesbery, W.R., Butterfield, D.A.

Notes: The authors used RQ1 RNase-Free DNase to remove contaminating genomic DNA from total RNA samples. Cu, Zn, and MN SOD mRNA sequences were amplified from hippocampal neuronal RNA using the Access RT-PCR System. (2218)

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J. Virol. 72, 2589-2599. Analysis of hepatitis C virus-inoculated chimpanzees reveals unexpected clinical profiles. 1998

Bassett, S.E., Brasky, K.M. and Lanford, R.E.

Notes: The Access RT-PCR System was used to determine the presence of hepatitis C virus RNA in chimpanzee serum. (1465)

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