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Blood 89, 2394-2403. Regulation of plasminogen gene expression by interleukin-6. 1997

Jenkins, G.R., Seiffert, D., Parmer, R.J., Miles, L.A.

Notes: Luciferase reporter studies were performed in Hep3B cells, and constructs were prepared in the pGL2-Basic and -Promoter Vectors. Luciferase activity was measured with the Luciferase Assay System. (0974)

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J. Mol. Neurosci. 8, 223-241. Regulation of the neural-specific gene VGF in PC12 cells: Identification of transcription factors interacting with NGF-responsive elements. 1997

Luc, P.V. and Wagner, J.A.

Notes: Reporter assays were performed in PC12 cells (1585)

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J. Clin. Invest. 99, 2906-2914. Regulation of the Rat Liver Sodium-dependent Bile Acid Cotransporter Gene by Prolactin:  Mediation of Transcriptional Activation by Stat5. 1997

Ganguly, T.C. , O'Brien, M.L. , Karpen, S.J. , Hyde, J.F. , Suchy, F.J. , Vore, M.

Notes: Luciferase studies were performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. (1137)

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J. Clin. Invest. 100, 972-985.. Role of NFκB in the mortality of sepsis 1997

Bohrer, H., Qiu, F., Zimmermann, T., Zhang, Y., Jllmer, T., Mannel, D., Bottiger, B.W., Stern, D.M., Waldherr, R., Saeger, H.D., Ziegler, R., Bierhaus, A., Martin, E. and Nawroth, P.P.

Notes: Intravenous somatic gene transfer with an expression plasmid coding for IκBα was used to investigate the role of members of the NFκB family in a mouse model of endotoxemia. Mouse kidneys were homogenized in 1X Cell Culture Lysis Reagent (CCLR) and assayed with the Luciferase Assay System. Reporter constructs were prepared in the pGL2 Basic and pGL2 Control Vectors. Please note the Anti-Luciferase pAb reported in the paper is different than the one currently sold by Promega. (1423)

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J. Biol. Chem. 272, 20691-20697.. Signaling from G protein-coupled receptors to the c-jun promoter involves the MEF2 transcription factor. Evidence for a novel c-jun amino-terminal kinase-independent pathway 1997

Coso, O. A. , Montaner, S. , Fromm, C. , Lacal, J. C. , Prywes, R. , Teramoto, H. , Gutkind, J. S.

Notes: Luciferase studies were performed in NIH3T3 cells expressing 20,000 muscarinic acetylcholine receptors per cell using constructs prepared in the pGL3 Promoter Vector. (1276)

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Mol. Pharmacol. 51, 250-261. Structural and functional characterization of the human alpha3 nicotinic subunit gene promoter. 1997

Fornasari, D., Battaglioli, E., Flora, A., Terzano, S. and Clementi, F.

Notes: Studies were performed in the neuroblastoma cells lines SY5Y and SK-N-BE and the human rhabdomyosarcoma cell line TE671. All reporter activities were reported as fold-stimulation over pGL3 Basic Vector basal expression. (1570)

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J. Biol. Chem. 272, 7501-7505. Structure and expression of H-type GDP-L-fucose:β-D-galactoside 2-α-L-fucosyltransferase gene (FUT1). Two transcription start sites and alternative splicing generate several forms of FUT1 mRNA. 1997

Koda, Y., Soejima, M., Kimura, H.

Notes: Luciferase studies were performed in MCAS ovarian cancer cells. Luciferase constructs were prepared in the pGL2-Enhancer Vector. (0915)

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J. Biol. Chem. 272, 17112-17117. Structure of the m1 muscarinic acetylcholine receptor gene and its promoter 1997

Pepitoni, S., Wood, I.C. Buckley, N.J.

Notes: The Dual-Luciferase® Reporter Assay System was used to study transfections of IMR32 and NIH3T3 cells with firefly luciferase vector (pGL3 Basic) constructs. Transfections were controlled with co-transfected pRL-CMV Vector. The promoter under study was truncated with the Erase-A-Base® System. Tth DNA Polymerase was used as a high temperature reverse transcriptase. SP6 RNA Polymerase was used to produce probes for RNase protection assays. (0558)

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J. Biol. Chem. 272, 14899-14907. Synergy between interferon-γ and tumor necrosis factor-α in transcriptional activation is mediated by cooperation between signal transducer and activator of transcription 1 and nuclear factor kappaB. 1997

Ohmori, Y. , Schreiber, R. D. , Hamilton, T. A.

Notes: Luciferase studies were performed in STAT1-deficient mouse fibroblasts and NIH3T3 cells using constructs prepared in the pGL2 Basic Vector. The luciferase activities were determined with the Luciferase Assay System. (0593)

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EMBO J. 16, 4007-4020. T-cell subset-specific expression of the IL-4 gene is regulated by a silencer element and STAT6. 1997

Kubo, M., Ransom, J., Webb, D., Hashimoto, Y., Tada, T., Nakayama, T.

Notes: Luciferase reporter studies were performed in BW5147 thymomas, EL-4 thymomas, 68-41 T-cell hybridomas, MS-SB cloned T-cells, P3U1 B-cells, L929 fibroblasts, PAM212 keratinocytes, and 1308-1 thymic epithelial cells using constructs prepared in the pGL2-Basic Vector. (0854)

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J. Biol. Chem. 272, 26285-26294. TGT3, thyroid transcription factor I, and Sp1 elements regulate transcriptional activity of the 1.3-kilobase pair promoter of t1alpha, a lung alveolar type I cell gene 1997

Ramirez, M.I., Rishi, A.K., Cao, Y.X., Williams, M.C.

Notes: Luciferase studies were performed in SV40 T type II cells and IMR90 fibroblasts using constructs prepared in the pGL3 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. The Erase-A-Base® System was used to generate deletion mutants of the promoter. The hepatocyte nuclear factor 3beta was produced in vitro with the TNT® Coupled Reticulocyte Lysate System and used for gel shift analysis. (0495)

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J. Neurosci. 17, 4149-4158. The characterization of the Olf-1/EBF-like HLH transcription factor family: Implications in olfactory gene regulation and neuronal development. 1997

Wang, S.S., Tsai, R.Y.L. and Reed, R.R.

Notes: The authors used Promega's pGL2 Basic Vector, pGL2 Promoter Vector and Luciferase Assay System. Studies on the Olf-1/EBF-like HLH transcription factor family were performed in human embryonic kidney 293 cells. (1548)

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J. Biol. Chem. 272, 1188-1196. The mitogen-activated protein kinase and JAK-STAT signaling pathways are required for an oncostatin M-responsive element-mediated activation of matrix metalloproteinase 1 gene expression. 1997

Korzus, E., Nagase, H., Rydell, R., Travis, J.

Notes: Luciferase studies were performed in normal human dermal fibroblasts and primary human astrocytes. The experimental constructs were prepared in the pGL2-Basic and pGL2-Promoter Vectors. All luciferase activities (determined with the Luciferase Assay System) were normalized to β-Galactosidase activity. (0884)

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J. Biol. Chem. 272, 13426-13431. The polymyositis-scleroderma autoantigen interacts with the helix-loop-helix proteins E12 and E47. 1997

Kho, C.J., Huggins, G.S., Endege, W.O., Patterson, C., Jain, M.K., Lee, M.E., Haber, E.

Notes: Luciferase reporter studies were performed in NIH3T3 cells. The researchers created sort of a two-hybrid pGL2 Vector to demonstrate that two cotransfected proteins can interact and bind an enhancer element, thus increasing luc activity. Researchers expressed one protein as a GST fusion and the other as a 35S-met protein in the TNT® Coupled Reticulocyte Lysate System and showed that the 35S-protein could be pulled out of the lysate. (0937)

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J. Biol. Chem. 272, 5579-5586. The promoters for human and monkey poliovirus receptors. Requirements for basic and cell type-specific activity. 1997

Solecki, D., Schwarz, S., Wimmer, E., Lipp, M., Bernhardt, G.

Notes: Luciferase studies were performed in L929, COS-1, HeLa, HEp2 and Raji Cells. Experimental constructs were prepared in the pGL2 Basic Vector and extracts were made with Reporter Lysis Buffer then assayed with the Luciferase Assay System. (0348)

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J. Biol. Chem. 272, 6979-6985. The rat arylalkylamine N-acetyltransferase gene promoter. cAMP activation via a cAMP-responsive element-CCAAT complex. 1997

Baler, R., Covington, S. and Klein, D.C.

Notes: Promega's Luciferase Assay System and pGL3 Basic Vector were used to study gene expression in primary rat pinealocytes. (1455)

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J. Biol. Chem. 272, 20522-20530. The rat pyruvate carboxylase gene structure. Alternate promoters generate multiple transcripts with the 5´-end heterogeneity. 1997

Jitrapakdee, S., Booker, G.W., Cassady, A.I., Wallace, J.C.

Notes: Luciferase reporter studies were performed in COS-1 cells using constructs prepared in the pGL3-Basic Vector. DNA sequencing was accomplished with the fmol® DNA Cycle Sequencing System. (0983)

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Proc. Natl. Acad. Sci. USA 94(16), 8812-8817. Transcription initiation is controlled by three core promoter elements in the hgl5 gene of the protozoan parasite Entamoeba histolytica. 1997

Singh, U., Rogers, J.B., Mann, B.J. and Petri, W.A., Jr.

Notes: Poly A+ RNA was isolated directly from E. histolytica using the PolyATtract® System 1000 and used for Northern and primer extension analysis. The RNAgents® Total RNA Isolation System was used to isolate total RNA from E. histolytica. The isolated RNA was used for nuclear run-on transcription. (1654)

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J. Biol. Chem. 272, 25890-25898. Transcription of human ABO histo-blood group genes is dependent upon binding of transcription factor CBF/NF-Y to minisatellite sequence. 1997

Kominato, Y., Tsuchiya, T., Hata, N., Takizawa, H., Yamamoto, F.I.

Notes: Luciferase studies were performed in KATO III human gastric cancer cells using constructs prepared in the pGL3-Basic and pGL3-Promoter Vectors. (0879)

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J. Biol. Chem. 272, 19575-19581. Transcriptional regulation of N-acetylglucosaminyltransferase V by the src oncogene. 1997

Buckhaults, P., Chen, L., Fregien, N., Pierce, M.

Notes: Luciferase studies were performed in HepG2 cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL2-Basic Vector. Transfection efficiency was standardized using the β-Galactosidase Assay System with Reporter Lysis Buffer. (2245)

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Blood 89, 2891-2900. Two distinct pathways of interleukin-5 synthesis in allergen-specific human T-cell clones are suppressed by glucocorticoids. 1997

Mori, A., Kaminuma, O., Suko, M., Inoue, S., Ohmura, T., Hoshino, A., Asakura, Y., Miyazawa, K., Yokota, T., Okumura, Y., Ito, K., Okudaira, H.

Notes: Luciferase reporter studies were performed in antigen-specific T cell clones. Experimental constructs were prepared in the pGL2 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. Nuclear extracts were then made from these T cell clones and used for gel shifts with the Gel Shift Assay System. The analysis included the use of the AP-1 Consensus Oligonucleotide, the NF-kappaB Consensus Oligonucleotide and the Oct-1 Consensus Oligonucleotide. (0661)

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J. Biol. Chem. 272, 17097-17103. Vasoactive peptides modulate vascular endothelial cell growth factor production and endothelial cell proliferation and invasion 1997

Pedram, A., Razandi, M., Hu, R. M., Levin, E. R.

Notes: Vascular smooth muscle cells were transfected with a firefly luciferase reporter vector and pRL-SV40 as a control. Transfection efficiency was normalized to the Renilla luciferase activity as determined by the Dual-Luciferase® Reporter Assay System. (0555)

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Endocrinology 137, 3897-3905. cis-acting elements and trans-acting proteins in the transcription of chorionic gonadotropin/luteinizing hormone receptor gene in human choriocarcinoma cells and placenta. 1996

Hu, Y.L., Lei, Z.M., Rao, C.V.

Notes: The authors used the Luciferase Assay System with Reporter Lysis Buffer (RLB) and the β-Galactosidase Assay System in JEG-3 human choriocarcinoma and HepG2 hepatoblastoma lines. (1024)

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Hum. Gene Ther. 7, 1205-1217. An improved plasmid DNA expression vector for direct injection into skeletal muscle. 1996

Hartikka, J., Sawdey, M., Cornefert-Jensen, F., Margalith, M., Barnhart, K., Nolasco, M., Vahlsing, H.L., Meek, J., Marquet, M., Hobart, P., Norman, J., Manthorpe, M.

Notes: pGL3-Control Vector and the Luciferase Assay System were used for in vivo studies on murine quadricep muscles by injection of DNA. Extracts for assaying the tissues were prepared using the CCLR. (1066)

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Hypertension 27, 709-714. Mechanisms of interleukin-1β regulation of nitric oxide synthase in cardiac myocytes. 1996

LaPointe, M.C., Sitkins, J.R.

Notes: Luciferase Assay System was used to study luciferase levels in cardiac myocytes. (0841)

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