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Mol. Pharmacol. 51, 963-971. DNA elements recognizing NF-Y and Sp1 regulate the human multidrug- resistance gene promoter. 1997

Sundseth, R., MacDonald, G., Ting, J., King, A. C.

Notes: Luciferase reporter studies were performed in HepG2 and HTC116 cells using constructs prepared in the pGL2 Basic Vector. Reporter activity was measured with the Luciferase Assay System. (0320)

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Mol. Pharmacol. 51, 703-710.. Effect of transforming growth factor-beta1 on expression of aryl hydrocarbon receptor and genes of Ah gene battery: clues for independent down-regulation in A549 cells. 1997

Dohr, O. , Sinning, R. , Vogel, C. , Munzel, P. , Abel, J.

Notes: The Transfectam® Reagent was used to transiently transfect A549 human lung cancer cells. Many details of the transfection are provided. Luciferase activity was normalized to Beta Galactosidase activity. (1240)

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J. Biol. Chem. 272, 16540-16547. Epidermal growth factor and okadaic acid stimulate Sp1 proteolysis. 1997

Mortensen, E.R., Marks, P.A., Shiotani, A., Merchant, J.L.

Notes: Used the Luciferase Assay System and the CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS) in their studies on GH4 rat pituitary adenoma cells. Reporter constructs were prepared in the pGL2 Basic Vector. (0667)

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Proc. Natl. Acad. Sci. USA 94, 7549-7554. Gestational exposure to ethanol suppresses msx2 expression in developing mouse embryos 1997

Rifas, L., Towler, D.A., Avioli, L.V.

Notes: Luciferase studies were performed on transfected MC3T3-E1 cell lysates using the Luciferase Assay System. Constructs were prepared in the pGL2 Promoter Vector. (0476)

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Am. J. Physiol. 273, G833-G841. Hepatocyte nuclear factor-1α regulates transcription of the guanylin gene. 1997

Hochman, J.A., Sciaky, D., Whitaker, T.L., Hawkins, J.A., Cohen, M.B.

Notes: Authors make 3 base changes in a promoter element using the Altered Sites® II in vitro Mutagenesis System and put the mutated promoter into the pGL3-Basic Vector. Luciferase activity was measured using the Luciferase Assay System. (1047)

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EMBO J. 16, 5943-5954.. High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression 1997

Costello, E. , Saudan, P. , Winocour, E. , Pizer, L. , Beard, P.

Notes: Luciferase studies were performed in 293T cells using constructs prepared in the pGL3 Basic Vector. (1278)

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J. Biol. Chem. 272, 8013-8018. Hyaluronan fragments induce nitric-oxide synthase in murine macrophages through a nuclear factor kappaB-dependent mechanism. 1997

McKee, C.M., Lowenstein, C.J., Horton, M.R., Wu, J., Bao, C., Chin, B.Y., Choi, A.M., Noble, P.W.

Notes: Reporter studies were performed in the mouse macrophage-like cell line RAW 264.7. Promoter constructs were prepared in the pGL2 Basic Vector and luciferase activity monitored with the Luciferase Assay System. Transfections were normalized by co-transfecting the pSV-β-Galactosidase Control Vector. The β-gal activity was assayed with the β-Galactosidase Assay System. (0713)

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J. Neurosci. 17, 7583-7593. Identification of a novel repressive element that contributes to neuron-specific gene expression. 1997

Weber, J.R.M. and Skene, J.H.P.

Notes: Reporter studies were performed in primary cultures of dissociated rat cerebral cortex and HTC hepatoma cells. The ratio of activity in neuronal cells to hepatoma cells was used as a measure of promoter specificity. Promega's pGL2 Basic Vector and Luciferase Assay System were used in these studies. (1549)

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J. Biol. Chem. 272, 2722-2728. Identification of a retinoid/chicken ovalbumin upstream promoter transcription factor response element in the human retinoid X receptor gamma2 gene promoter. 1997

Barger, P.M. and Kelly, D.P.

Notes: The Luciferase Assay System and the pGL2 Basic Vector were used to study gene expression in CV-1 cells. (1460)

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J. Biol. Chem. 272, 10367-10371. Identification of an element required for acetylcholine receptor- inducing activity (ARIA)-induced expression of the acetylcholine receptor epsilon subunit gene. 1997

Si, J., Miller, D. S., Mei, L.

Notes: Luciferase studies were performed in C2C12 myoblasts. Experimental constructs were prepared in the pGL2 Basic Vector, and luciferase activities were determined with the Luciferase Assay System. (0403)

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Virology 238, 432-443. Increasing the ratio of PP2A core enzyme to holoenzyme inhibits Tat-stimulated HIV-1 transcription and virus production. 1997

Ruediger, R., Brewis, N., Ohst, K. and Walter, G.

Notes: This paper describes a method for quantitation of luciferase mRNA by in vitro translation using Promega TNT® Coupled Wheat Germ Extract System. (Rabbit reticulocyte lysate could not be used because of luciferase quenching problems). In this reporter gene assay, COS cells were transfected with a firefly luciferase reporter plasmid driven by promoters/enhancers of varying strengths and total cellular RNA was isolated and translated in vitro using the TNT® System. To normalize expression, a CMV Renilla luciferase or beta-galactosidase vector was used as a control. To measure luciferase activity either Promega Luciferase Assay System or Dual-Luciferase® Reporter Assay System was used. (2047)

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Am. J. Physiol. 273, L1090-L1095. Induction of aquaporin 3 by corticosteroid in a human airway epithelial cell line. 1997

Tanaka, M., Inase, N., Fushimi, K., Ishibashi, K., Ichioka, M., Sasaki, S. and Marumo, F.

Notes: A 318bp promoter region from genomic DNA was amplified by PCR and ligated into the pGEM®-T Vector. This promoter region was then cloned into the pGL2-Basic Vector, and activity was measured using the Luciferase Assay System. Transfection efficiency was monitored with the β-Galactosidase Enzyme Assay System. (0300)

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J. Biol. Chem. 272, 791-797. Inhibition of myogenesis by multiple cyclin-Cdk complexes. Coordinate regulation of myogenesis and cell cycle activity at the level of E2F. 1997

Guo, K., Walsh, K.

Notes: Perform luciferase studies in C2C12 myocytes and 10T1/2 cells using constructs prepared in the pGL2-Basic Vector. Luciferase activity was normalized to soluble alkaline phosphatase control activity. (1087)

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J. Clin. Invest. 100, 1797-1803. Interleukin 6 is autoregulated by transcriptional mechanisms in cultures of rat osteoblastic cells. 1997

Franchimont, N. , Rydziel, S. , Canalis, E.

Notes: Luciferase studies were performed in primary rat osteoblasts using both the pGL2 Basic and pGL3 Basic vectors. (1168)

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J. Biol. Chem. 272, 15003-15010. Isolation and characterization of the human gp130 promoter. Regulation by STATS. 1997

O'Brien, C. A. , Manolagas, S. C.

Notes: Luciferase studies performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-Galactosidase Control Vector to normalize for transfection efficiency. Luciferase activities were measured with the Luciferase Assay System. (0629)

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J. Biol. Chem. 272, 19777-19784.. Localization of the major NFκB-activating site and the sole TRAF3 binding site of LMP-1 defines two distinct signaling motifs. 1997

Brodeur, S.R., Cheng, G., Baltimore, D., Thorley-Lawson, D.A.

Notes: The pCI-Neo Mammalian Expression Vector was used in conjunction with a luciferase reporter vector to determine the important regions on the carboxy-terminal portion of the 386-amino acid LMP-1 protein. Both full-length and truncation mutants were expressed in the BJAB human EBV-negative lymphoblastoid B cell line. Luciferase activity was measured using the Luciferase Assay System. (1409)

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J. Biol. Chem. 272, 13683-13689. Lysophosphatidylcholine stimulates activator protein 1 and the c-Jun N-terminal kinase activity. 1997

Fang, X. , Gibson, S. , Flowers, M. , Furui, T. , Bast, R. C. Jr, Mills, G. B.

Notes: Luciferase assays were performed in Swiss 3T3 cells. Researchers replaced CAT in their old vectors with the luc gene from pGL2 Basic. (1193)

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EMBO J. 16, 5483-5490. Mammalian protein RAP46: an interaction partner and modulator of 70 kDa heat shock proteins 1997

Zeiner, M. , Gebauer, M. , Gehring, U.

Notes: Calf Intestinal Alkaline Phosphatase was used for in vitro dephosphorylation of the RAP46 protein, and the dephosphorylated protein was assayed for interaction with various transcription factors. The TNT® Coupled Reticulocyte Lysate System was used to produce the transcription factors. The Rabbit Reticulocyte Lysate was used to aid protein refolding of heat denatured luciferase and the extent of refolding was examined with the Luciferase Assay System. (0084)

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J. Biol. Chem. 272, 20502-20507. Murine laminin alpha3A and alpha3B isoform chains are generated by usage of two promoters and alternative splicing 1997

Ferrigno, O. , Virolle, T. , Galliano, M. F. , Chauvin, N. , Ortonne, J. P. , Meneguzzi, G. , Aberdam, D.

Notes: Authors performed luciferase studies in PAM212 mouse keratinocytes and NIH3T3 cells using constructs prepared in the pGL2 Basic and pGL2 Promoter Vectors. (1152)

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J. Biol. Chem. 272, 18564-18571. Mutational analysis of the murine granzyme B gene promoter in primary T cells and a T cell clone. 1997

Babichuk, C.K. and Bleackley, R.C.

Notes: The pGL2 Basic Vector and the pSV β-Galactosidase Control Vector were used in conjunction with the Luciferase Assay System to study gene regulation in primary mouse lymphocytes and the murine CTL line MTL 2.8.2. (1487)

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EMBO J. 16, 4276-4284.. Nuclear export of the E1B 55-kDa and E4 34-kDa adenoviral oncoproteins mediated by a rev-like signal sequence. 1997

Dobbelstein, M. , Roth, J. , Kimberly, W. T. , Levine, A. J. , Shenk, T.

Notes: CAT reporter activity was normalized to luciferase activity using the Luciferase Assay System. Luciferase was provided by the pGL3 Control Vector and CAT activity was measured using the CAT Enzyme Assay System. (1237)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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J. Biol. Chem. 272, 17802-17809. PU.1 Is Essential for p47(phox) Promoter Activity in Myeloid Cells. 1997

Li, S.L., Valente, A.J., Zhao, S.J., Clark, R.A.

Notes: Luciferase studies were performed in HL-60 cells. Experimental constructs were prepared in pGL3 Basic Vector and luciferase activities determined with the Luciferase Assay System. (0796)

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J. Biol. Chem. 272, 26620-26626. Regulation of clusterin gene expression by transforming growth factor β. 1997

Jin, G., Howe, P.H.

Notes: Luciferase reporter studies performed in CCL64 (Mv1Lu) mink lung cells using constructs prepared in the pGL2-Basic Vector and were normalized to β-galactosidase activity. Studies performed in 10T1/2, 3Tp, HeLa cells and primary bovine aortic endothelial cells were normalized to the Renilla luciferase. A five to one (w/w) ratio of experimental to control vector was used with pRL-CMV Control Vector. A two to one (w/w) ratio of experimental to control vector was used with pSV-β-Galactosidase Control Vector. (0981)

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Clin. Exp. Hypertens. 19, 543-550. Regulation of human renin gene transcription by cAMP. 1997

Germain, S., Konoshita, T., Fuchs, S., Philippe, J., Corvol, P. and Pinet, F.

Notes: Authors performed luciferase assays using the pGL3 Basic Vector in Calu-6 cells. (1151)

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