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J. Biol. Chem. 273, 32312-32321. Repression of cyclooxygenase-2 and prostaglandin E2 release by dexamethasone occurs by transciptional and post-transcriptional mechanisms involving loss of polyadenylated mRNA 1998

Newton, R., Seybold, J., Kuitert, L.M.E., Bergmann, M. , Barnes, P.J.

Notes: A reporter construct was assembled in the pGL3 Control Vector downstream of the Xba I consisting of the 3'UTR of the COX-2 gene. The construct was designed to see if the UTR had an affect on mRNA stability in the presence of IL-1beta. The construct as well as the pSV-Beta Galactosidase Control Vector were transfected into A549 cells using the Tfx™-50 Reagent. A lot of detail is provided for the transfection. The reporter activities were monitored with the Luciferase Assay System and the Beta-Galactosidase Enzyme Assay System. (0606)

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J. Biol. Chem. 273, 26218-26224. Synergistic activation of the N-methyl-D-aspartate receptor subunit 1 promoter by myocyte enhancer factor 2C and Sp1. 1998

Krainc, D., Bai, G., Okamoto, S., Carles, M., Kusiak, J.W., Brent, R.N., Lipton, S.A.

Notes: Luciferase (prepared in pGL2-Basic Vector) and β-galactosidase (pSV-β-Galactosidase Control Vector) reporters were studied in SL2 Drosophila cells, HeLa cells and primary rat cortical neurons. Transfections into the SL2 and HeLa cells were accomplished with standard calcium phosphate methods. Transfection of primary rat cortical neurons was accomplished with the Tfx™-50 Reagent as described in Boeckman, F.A. et al. (1996) Neural Notes II(1), 13-15. Reporter assays were performed with the Luciferase Assay System and the β-Galactosidase Assay System. (0890)

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J. Med. Chem. 41, 2207-2215. Synthesis and characterization of long chain alkyl acyl carnitine esters: Potentially biodegradable cationic lipids for use in gene delivery systems. 1998

Wang, J., Guo, X., Xu, Y., Barron, L. and Szoka, Jr., F.C.

Notes: DNA-liposomes were injected intraveneously with a RSV-luciferase expression vector. Forty-eight hours post injection, the animal was sacrificed and the heart, lung and ~300mg of liver was homogenized in Promega's Luciferase Cell Culture Lysis Reagent with the aid of zirconium beads. The debris was centrifuged away and the supernatant analyzed for luciferase activity with the Luciferase Assay System. (0199)

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J. Biol. Chem. 273, 26923-26930. The early growth response protein (EGR-1) regulates interleuking-2 transcription by synergistic interaction with the nuclear factor of activated T cells. 1998

Decker, E.L., Skerka, C., Zipfel, P.F.

Notes: The 293 cell line was cotransfected with a luciferase reporter containing the IL-2 promoter, an expression vector for either EGR-1, NFATc or both and finally the pRL-TK Vector. Reporter to control ratio was 4:1 and the luciferase activity was determined with the Dual-Luciferase® Reporter Assay System. (1265)

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J. Biol. Chem. 273, 5607-5614. Transcriptional activation of the UDP-glucuronosyltransferase 1A7 gene in rat liver by aryl hydrocarbon receptor ligands and oltipraz. 1998

Metz, R.P. and Ritter, J.K

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

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J. Biol. Chem. 273, 14885-14890. Transcriptional regulation of endothelial nitric-oxide synthase by lysophosphatidylcholine 1998

Cieslik, K., Zembowicz, A., Tang, J.-L., Wu, K.K.

Notes: The Serine/Threonine Phosphatase Assay System was used to assess protein phosphatase 2A, 2B and 2C activity in nuclear extracts of Human umbilical vein cells (HUVEC) with or without lysophosphatidylcholine treatment. Gel shifts were also performed with nuclear extracts prepared from the HUVEC cells using the SP1 Consensus Oligonucleotide. Luciferase reporter studies were also performed in HUVEC cells using pGL3 Basic-derived vectors and the Luciferase Assay System. (1305)

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J. Immunol. 161, 3010-3018. Two distinct phospholipases C of Listeria monocytogenes induce ceramide generation, nuclear factor-kappaB activation, and E-selectin expression in human endothelial cells. 1998

Schwarzer, N., Nöst, R., Seybold, J., Parida, S.K., Fuhrmann, O., Krüll, M., Schmidt, R., Newton, R., Hippenstiel, S., Domann, E., Chakraborty, T., Suttorp, N.

Notes: A NF-kappaB luciferase reporter vector was constructed with the TATA box and transcription start site of the rabbit beta-globin promoter and three tandem repeats of the NF-kappaB consensus site in the pGL3 Basic Vector. Another vector was constructed with mutated NF-kappaB sites. The reporter vector was transfected into primary human umbilical vein endothelial cells with the Tfx™-50 Reagent. No details of the transfection conditions were provided. Luciferase activities were monitored with the Luciferase Assay System. (0418)

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J. Neurosci. 17, 2273-2283. β43´: an enhancer displaying neural-restricted activity is located in the 3´-untranslated exon of the rat nicotinic acetylcholine receptor β4 gene. 1997

McDonough, J., Deneris, E.

Notes: Reporter studies were performed mainly in PC12 cells pGL2-Basic, pGL2-Promoter and pGL2-Control Vectors. Neuronal specificity was assayed in PC12, HeLa, Rat2 (rat fibroblast), Clone 9 (rat liver), ARIP (rat pancreatic tumor), C1300 (rat neuroblastoma) and rat keratinocytes. Luciferase activity was monitored with the the Luciferase Assay System. (0710)

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J. Biol. Chem. 272, 18490-18497.  Two distinct factor-binding DNA elements in cardiac myosin light chain 2 gene are essential for repression of its expression in skeletal muscle.  Isolation of a cDNA clone for repressor protein Nished. 1997

Dhar, M. , Mascareno, E.M. , Siddiqui, M.A.Q.

Notes: Luciferase studies were performed in primary chicken skeletal muscle cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL2 Basic and pGL2 Promoter Vectors. (1229)

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J. Biol. Chem. 272, 10551-10557.. A common pro-opiomelanocortin-binding element mediates leukemia inhibitory factor and corticotropin-releasing hormone transcriptional synergy. 1997

Bousquet, C. , Ray, D. W. , Melmed, S.

Notes: Luciferase studies were performed in AtT20 cells using the Luciferase Assay System. Experimental constructs were prepared in the pGL3 Basic Vector. (1392)

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EMBO J. 16, 659-671. A small heat shock protein stably binds heat-denatured model substrates and can maintain a substrate in a folding-competent state. 1997

Lee, G. J. , Roseman, A. M. , Saibil, H. R. , Vierling, E.

Notes: The Quantilum™ Recombinant Luciferase was heat denatured in the presence of heat shock protein hsp 18.1 and the renaturation of the protein was assayed in with the Luciferase Assay System. The denaturation was performed in either Rabbit Reticulocyte Lysate or Wheat Germ Extract in the presence or absence of added ATP. (0809)

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J. Biol. Chem. 272, 21045-21051. A type I interferon signaling factor, ISF21, encoded on chromosome 21 is distinct from receptor components and their down-regulation and is necessary for transcriptional activation of interferon-regulated genes. 1997

Holland, K.A., Owczarek, C.M., Hwang, S.Y., Tymms, M.J., Constantinescu, S.N., Pfeffer, L.M., Kola, I., Hertzog, P.J.

Notes: Luciferase and CAT assays were conducted in CHO-K1 cells using constructs prepared in the pGL3-Basic and pCAT®-Basic Vectors, and the activity was normalized to β-galactosidase activity supplied by the pSV-β-Galactosidase Control Vector. The pCAT® Vectors have been replaced by the next-generation pCAT®3 Vectors. (1055)

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Proc. Natl. Acad. Sci. USA 94, 7543-7548. Angiotensin II type1a receptor gene expression in the heart: AP-1 and GATA-4 participate in the response to pressure overload. 1997

Herzig, T.C., Jobe, S.M., Aoki, H., Molkentin, J.D., Cowley, A.W., Jr., Izumo, S., Markham, B.E.

Notes: Luciferase studies were performed in primary rat cardiac ventricular myocytes using constructs prepared in the pGL2-Basic Vector. (1041)

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J. Biol. Chem. 272, 255-261. Arginine-specific regulation mediated by the Neurospora crassa arg-2 upstream open reading frame in a homologous, cell-free in vitro translation system. 1997

Wang, Z. , Sachs, M. S.

Notes: Luciferase reporter constructs containing either the luc or luc+ gene (from pSP-luc+NF Fusion Vector) were used in in vitro transcription to produce capped mRNA. Luciferase was produced by in vitro translation using either neurospora extract or Promega's Rabbit Reticulocyte Lysate, Nuclease Treated and detected using the Luciferase Assay Reagent. (0208)

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J. Biol. Chem. 272, 18316-18324. Binding of upstream stimulatory factor and a cell-specific activator to the calcitonin/calcitonin gene-related peptide enhancer. 1997

Lanigan, T.M., Russo, A.F.

Notes: Luciferase studies were performed in CA77 thyroid C cell line UI-0 using constructs prepared in the pGL3-Basic Vector. (0838)

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Blood 90, 2784-2795. CCAAT displacement protein (CDP/cut) recognizes a silencer element within the lactoferrin gene promoter. 1997

Khanna-Gupta, A., Zibello, T., Kolla, S., Neufeld, E.J., Berliner, N.

Notes: Luciferase reporter studies were performed in K562 and HEL human erythroleukemia cells using constructs prepared in the pGL2-Basic Vector. (0936)

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J. Biol. Chem. 272, 8581-8593. Characterization of a subset of the basic-helix-loop-helix-PAS superfamily that interacts with components of the dioxin signaling pathway. 1997

Hogenesch, J.B., Chan, W.K., Jackiw, V.H., Brown, R.C., Gu, Y.Z., Pray-Grant, M., Perdew, G.H., Bradfield, C.A.

Notes: Luciferase studies were performed in Hep3b cells. Experimental constructs were prepared in the pGL2-Promoter Vector and luciferase activities determined with the Luciferase Assay System. (1052)

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J. Biol. Chem. 272, 12809-12815.. Characterization of retinoic acid-induced AP-1 activity in B16 mouse melanoma cells. 1997

Desai, S. H. , Niles, R. M.

Notes: In this paper, the pGL2 Basic Vector, the pSV-Beta Galactosidase Control Vector and the Luciferase Assay System were used to study gene expression in B16 mouse melanoma cells. (1271)

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Proc. Natl. Acad. Sci. USA 94, 9487-9492. Characterization of the α1B-adrenergic receptor gene promoter region and hypoxia regulatory elements in vascular smooth muscle. 1997

Eckhart, A.D. , Yang, N. , Xin, X. , Faber, J.E.

Notes: Luciferase studies were performed in aortic and vena caval smooth muscle cells. Transfections were normalized to β-galactosidase activity. Researchers report that pGL3 Promoter and pGL3 Control Vectors were 55-± 3-fold and 340±41-fold greater activity, respectively, than the pGL3 Basic Vector alone. The CREB consensus oligo was used in gel shifts of aortic and vena cava smooth muscle cell nuclear extracts. (1214)

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J. Biol. Chem. 272, 14365-14371. cis-Acting elements and trans-acting proteins in the transcriptional inhibition of gonadotropin-releasing hormone gene by human chorionic gonadotropin in immortalized hypothalamic GT1-7 neurons. 1997

Lei, Z. , Rao, C. V.

Notes: Luciferase studies were performed in GT1-7 immortalized neurons using construct assembled in the pGL2 Basic Vector. Luciferase activity was measured with the Luciferase Assay System. (0823)

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J. Biol. Chem. 272, 12692-12698.. Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein. 1997

Chatterjee, N. , Zou, C. , Osterman, J. C. , Gupta, N. K.

Notes: Luciferase studies were performed in KRC-7 rat hepatoma cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-galactosidase Vector. Luciferase activities determined with the Luciferase Assay System were normalized to readings from the Beta-Galactosidase Enzyme Assay System. (1364)

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J. Neurosci. 17, 4159-4169. Cloning and functional characterization of Roaz, a zinc finger protein that interacts with O/E-1 to regulate gene expression: implications for olfactory neuronal development. 1997

Tsai, R. Y. , Reed, R. R.

Notes: Reporter studies were performed in human embryonic kidney 293 cells using constructs prepared in the pGL2-Basic Vector. The Erase-A-Base® System was used to create unidirectional deletion mutants of promoter elements. (0236)

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J. Biol. Chem. 272, 7445-7454. Constitutive and beta-naphthoflavone-induced expression of the human gamma-glutamylcysteine synthetase heavy subunit gene is regulated by a distal antioxidant response element/TRE sequence. 1997

Mulcahy, R.T., Wartman, M.A., Bailey, H.H., Gipp, J.J.

Notes: Reporter studies were performed in HepG2 cells. The experimental constructs were made using the the pGL3 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. (0669)

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EMBO J. 16, 406-416. Deficient cytokine signaling in mouse embryo fibroblasts with a targeted deletion in the PKR gene: role of IRF-1 and NF-kappaB. 1997

Kumar, A., Yang, Y.L., Flati, V., Der, S., Kadereit, S., Deb, A., Haque, J., Reis, L., Weissmann, C., Williams, B.R.

Notes: Luciferase studies were performed in mouse embryo fibroblasts. Experimental constructs were prepared in the pGL2-Basic Vector and luciferase activities determined with the Luciferase Assay System. (0861)

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J. Neurosci. 17, 6554-6564.. Differential expression of alpha-bungarotoxin-sensitive neuronal nicotinic receptors in adrenergic chromaffin cells: A role for transcription factor Egr-1. 1997

Criado, M., Dominguez del Toro, E.D., Carrasco-Serrano, C., Smillie, F.I., Juíz, J.M., Viniegra, S. and Ballesta, J.J.

Notes: Total RNA was isolated from adrenomedullary tissue dissected from areas near to or far from the adrenal cortex. The isolated RNA was used for RT-PCR. Reporter studies were performed in Neuro2a murine neuroblastomas, SH-SY5Y human neuroblastomas and bovine chromaffin cells. All luciferase activities were normalized to control beta-Galactosidase activity. The consensus oligonucleotides were used in gel shift assays of bovine chromaffin cell nuclear extracts. The oligonucleotides were used to show specificity for Egr-1. (1614)

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