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Neuroscience 91, 1027-1034. Expression of nerve growth factor in astrocytes of the hippocampal CA1 area following transient forebrain ischemia. 1999

Gottlieb, M., and Matute, C.

Notes: The NGF EMAX® ImmunoAssay System (NGF ELISA) was used to assay for NGF protein levels in the CA1 region of the hippocampus from rats experiencing a transient ischemia and control animals. References and detail of the tissue preparation is provided. (1113)

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Eur. J. Neurosci. 11, 1668-1676. The role of p75NTR in modulating neurotrophin survival effects in developing motoneurons. 1999

Wiese, S., Metzger, F., Holtmann, B., Sendtner, M.

Notes: The BDNF Emax® System; the NGF Emax® System and the NT-3 Emax® System were each used to quantitate how much of BDNF, NGF and NT-3, respectively, were put into cultures of wildtype and p75NTR-deficient mouse motoneuron cultures. (0186)

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J. Neurosci. 18, 9326-9334. Dexamethasone induces hypertrophy of developing medial septum cholinergic neurons: Potential Role of nerve growth factor. 1998

Shi, B., Rabin, S.J., Brandoli, C., Mocchetti, I.

Notes: The NGF Emax® ImmunoAssay System was used to determine the level of NGF protein in different portions of the rat brain. Excellent detail is provided for sample preparation. The rats were treated for one week prior to assay with either vehicle or dexamethasone. NGF levels were normalized to the amount of protein in the extracts. (0390)

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Am. J. Respir. Crit. Care Med. 158 (6), 2002-2005. Neurotrophins are increased in bronchoalveolar lavage fluid after segmental allergen provocation. 1998

Virchow, J.C., Julius, P., Lommatzsch, M., Luttmann, W., Renz, H. and Braun, A.

Notes: Bronchoalveolar lavage fluid samples from human patients were analyzed with the BDNF, NT-3 and NGF Emax® ImmunoAssay Systems. Details of the lavage procedure are provided.  BDNF, NT-3 and NGF data were expressed as an average of duplicates in picograms per milliliter.  (2740)

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Proc. Natl. Acad. Sci. USA 95, 7784-7788. Pancreatic beta cells synthesize and secrete nerve growth factor 1998

Rosenbaum, T., Vidaltamayo, R., Sanchez-Soto, M.C., Zentella, A., Hiriart, M.

Notes: The NGF Emax® ImmunoAssay System was used to measure NGF released into conditioned media of cultured rat pancreatic beta cells. The amount of NGF released increased with glucose treatment of the cells. The release was not inhibited by KCl but was inhibited by dibutryl cAMP. (0455)

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Eur. J. Immunol. 28, 3240-3251. Role of nerve growth factor in a mouse model of allergic airway inflammation and asthma. 1998

Braun, A., Appel, E., Baruch, R., Herz, U., Botchkarev, V., Paus, R., Brodie, C., Renz, H.

Notes: The NGF Emax® ImmunoAssay System was used to determine the NGF content of mouse lavage and serum as well as the conditioned media of mononuclear cells. Allergic conditions showed increases in NGF. (1400)

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Exp. Neurol. 145(2 Pt 1), 592-6. An acid-treatment method for the enhanced detection of GDNF in biological samples 1997

Okragly, A.J. and Haak-Frendscho, M.

Notes: The levels of GDNF, NGF, and NT-3 were quantitated in various mouse tissues, including spleen, muscle, heart, lung, liver, kidney, bladder, gastrointestinal tract, brain, and serum. Additional samples screened for NGF included pig, goat, horse, chicken, rabbit, sheep, and bovine serum and rat brain. Samples were untreated or acid treated by acidification to pH <3.0 followed by neutralization to pH 7.6. In all tissue samples and most serum samples, the detectable levels of GDNF, NGF and NT-3 increased up to 35-fold. It is thought that the acidification process promotes dissociation of receptors and associated proteins making the neurotrophins more accessible. Levels of GDNF, NGF, and NT-3 were determined using the GDNF, NGF, and NT-3 Emax ImmunoAssay Systems, respectively.  Please note that the coat antibody in the NGF Emax ImmunoAssay System was changed 12/2002 and may affect cross-reactivity. (3105)

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