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J. Immunol. 166, 7238-7243. Processing of immunosuppressive pro-TGF-beta 1,2 by human glioblastoma cells involves cytoplasmic and secreted furin-like proteases. 2001

Leitlein, J., Aulwurm, S., Waltereit, R., Naumann, U., Wagenknecht, B., Garten, W., Weller, M., and Platten, M.

Notes: Inhibitors of furin-like protease activity interfere with processing and secretion of TGFβ1 and β2 from cells. Levels of TGFβ1 released into spent culture medium of SV40 large T-Ag immortalized fetal human astrocytic cell line Sv-FHAS and the human glioma cell lines LN-18 and T98G were determined by Western blot analysis using the Anti-TGFβ1 pAb (1:2000 dilution). Due to the low protein concentrations, the spent medium was concentrated with the Centriplus centrifugal filter device YM-3 (Millipore) prior to SDS-PAGE. (2455)

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Anti-TGFβ1 pAb

J. Clin. Endocrinol. Metab. 85, 847-852. Androgen influences transforming growth factor-beta1 gene expression in human adrenocortical cells. 2000

Zatelli, M.C., Rossi, R., and degli Uberti, E.C.

Notes: The effects of dihydrotestosterone stimulation on TGFβ1 production by the androgen sensitive human adrenocortical cell line, NCI-H295 was determined. DHT upregulated TGFβ1 protein levels in conditioned media of NCI-H295; in turn TGFβ1 significantly reduced cell proliferation. Bioneutralization with the Anti-TGFβ1 pAb (0.2 µg/ml) blocked the inhibitory effect of TGFβ1 (10-9 to 10-6 mol/L) on NCI-H295 cell proliferation. Production and release of biologically active TGFβ1 by NCI-H295 cells was monitored by ELISA using the TGFβ1 Emax® Immunoassay System (2458)

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Cancer Res. 60, 4531-37. Transforming growth factor-beta1 recruits histone deacetylase 1 to a p130 repressor complex in transgenic mice in vivo. 2000

Bouzahzah, B., Fu, M., Iavarone, A., Factor, V.M., Thorgeirsson, S.S., and Pestell, R.G.

Notes: The authors find that TGF-β1 may regulate interactions between histone deacetylase and the p130 protein, shedding more light on the involvement of chromatin remodeling proteins in oncogenesis. Transgenic mice overexpressing hepatic TGF-β1 were used to study the relationship between levels of TGFβ1 and the histone deacetylase/p130 complex. To monitor TGFβ1 levels, hepatic tissue extracts were subjected to Western blot analysis using the Anti-TGFβ1 pAb. (2456)

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Anti-TGFβ1 pAb

J. Biol. Chem. 274, 9984-9992. Assignment of transforming growth factor beta1 and beta3 and a third new ligand to the type receptor ALK-1. 1999

Lux, A., Attisano, L., Marchuk, D.A.

Notes: The Anti-TGF-β1 pAb was used to neutralize TGF-β1 in cell culture media supplemented with either 4ng/ml TGF-β1 or 20% serum. The antibody at 1µg/ml inhibited the 4ng/ml TGF-beta1 by about 50%. (0756)

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Anti-TGFβ1 pAb

Proc. Natl. Acad. Sci. USA 96, 2345-2349. Blockade of type beta transforming growth factor signaling prevents liver fibrosis and dysfunction in the rat. 1999

Qi, Z., Atsuchi, N., Ooshima, A., Takeshita, A., Ueno, H.

Notes: Authors use the Anti-TGFβ1 pAb for immunohistochemistry (IHC) on rat livers. (0523)

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Anti-TGFβ1 pAb

J. Immunol. Methods 225, 87-93. Isoform specificity of commercially-available anti-TGF-beta antibodies. 1999

Mozes, M.M., Hodics, T. and Kopp. J.B.

Notes: The Anti-TGFβ1 pAb was used in Western blots and compared with three other anti-TGF antibodies. When the Western results were analyzed by band densitometry,  Anti-TGFβ1 pAb displayed 0.3% cross-reactivity to reduced TGFβ2 and TGFβ3. The researchers determined that Western blots performed with the Anti-TGFβ1 pAb were sensitive to 50pg of human recombinant TGFβ1. (2766)

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Anti-TGFβ1 pAb

Proc. Natl. Acad. Sci. USA 96(6), 3087-91. Transforming growth factor beta stimulation of colorectal cancer cell lines: type II receptor bypass and changes in adhesion molecule expression. 1999

Ilyas, M., Efstathiou, J.A., Straub, J., Kim, H.C., and Bodmer, W.F.

Notes: The presence of type II TGFβ receptor mutations in 12 colorectal cancer cell lines (LS174T, HCA7, LOVO, SW48, DLD1, HCT116, COLO201, COLO205, COLO320, HCA46, HT29, and CACO2) was examined. Six cell lines were found to have homozygous mutations. Growth inhibition upon TGFβ1 stimulation and changes in adhesion molecule expression of these cell lines was also studied. Cells were stimulated with Promega's human TGFβ1 at a concentration of 2.5 ng/ml and cell proliferation was measured using the CellTiter 96® AQueous Nonradioactive Cell Proliferation Assay. Two cell lines were shown to be inhibited by TGFβ1 despite homozygous mutations in the type II TGFβ receptor gene. As a control, TGFβ1 was bioneutralized with the Anti-TGFβ1 pAb at a 2:1 molar ratio of antibody to TGFβ1. (2457)

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Gastroenterology 114, 940-946.. Short-chain fatty acids induce cell cycle inhibitors in colonocytes 1998

Wang, J., Friedman, E.A.

Notes: The Anti-TGFβ1 pAb (1:5,000 dilution) was used to probe U4 human colon carcinoma cell extracts by Western blotting following treatment of the cells with short chain fatty acids. (0202)

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Anti-TGFβ1 pAb

Cell Growth Differ. 8, 101-11. Fenretinide: Induction of apoptosis and endogenous transforming growth factor beta in PC-3 prostate cancer cells. 1997

Roberson, K.M., Penland, S.N., Padilla, G.M., Selvan, R.S., Kim, C.S., Fine, R.L., and Robertson, C.N.

Notes: The authors investigate the antineoplastic properties of fenretinide and try to correlate an increase in TGFβ1 expression with the induction of apoptosis in the human prostate cancer cell line PC3. Expression of TGFβ1 in PC3 cells was examined by immunocytochemistry. Cells were fixed in acetone, blocked with 5% goat serum, and stained with the Anti-TGFβ1 pAb (1µg/ml ). (2454)

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Anti-TGFβ1 pAb

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