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Duncan, R.R., Westwood, P.K., Boyd, A. and Ashley, R.H.
Notes: The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA.The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid. (1206)
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pCI Mammalian Expression Vector
pGEM®-T Vector System I
pGEM®-T Vector System II
TnT® SP6 Coupled Reticulocyte Lysate System
TnT® SP6 Coupled Reticulocyte Lysate System, Trial Size
TnT® T3 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System, Trial Size
TnT® T7/SP6 Coupled Reticulocyte Lysate System
TnT® T7/T3 Coupled Reticulocyte Lysate System
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Koda, Y., Soejima, M., Kimura, H.
Notes: Luciferase studies were performed in MCAS ovarian cancer cells. Luciferase constructs were prepared in the pGL2-Enhancer Vector. (0915)
Luciferase Assay System
Schäfer, M., Schütz, B., Weihe, E. and Eiden, L.
Notes: The pGEM®-T Vector System was used to clone a 270bp RT-PCR product from rat spinal cord mRNA.. (2007)
Mitsuda, N., Roses, A.D. and Vitek, M.P.
Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)
Dual-Luciferase® Reporter Assay System
Wizard® PCR Preps DNA Purification System
Ninkina, N. , Grashchuck, M. , Buchman, V. L. , Davies, A. M.
Notes: The PolyATtract® System 1000 was used to isolate polyA+ RNA directly from newborn mouse brains. The RNA was used in RT-PCR for detection of specific TrkB variants. Taq DNA Polymerase and the pGEM®-T Vector System were used to produce and subclone novel TrkB variants that differ in the extracellular domain. The CellTiter 96® Non-Radioactive Cell Proliferation Assay was performed on NIH 3T3 cells transfected with the TrkB receptors and challenged with BDNF, NT3 and NT4/5. (0615)
CellTiter 96® Non-Radioactive Cell Proliferation Assay
PolyATtract® System 1000 with Magnetic Stand
PolyATtract® System 1000 without Magnetic Stand
Perry, D. and Furnier, G.
Notes: The pGEM®-T Vector System was used in this study to clone amplified cDNAs. (1973)
Laville, M., Auboeuf, D., Khalfallah, Y., Vega, N., Riou, J.P. and Vidal H.
Notes: Promega's Tth DNA Polymerase, pGEM®-T Vector System and Riboprobe® System were used in this study. (2002)
Riboprobe® Combination System-SP6/T7 RNA Polymerase
Riboprobe® Combination System-T3/T7 RNA Polymerase
Tth DNA Polymerase
Guo, J., Aldrich, C., Mason, W. and Fox, J.
Notes: The pGEM®-T Vector System was used in this study. (2194)
Zardoya, R. , Abouheif, E. , Meyer, A.
Notes: PCR products were cloned into the pGEM®-T Vector System. (0082)
Forleo, P., Couchie, D., Chabas, S. and Nunez, J.
Notes: The system was used to clone the amplimers generated by RT-PCR. Sequencing of the clones was performed in the vector. (1569)
Roche, J. van der Staay, G., Partensky, F., Ducret, A., Aebersold, R., Li, R., Golden, S., Hiller, R., Wrench, R., Larkum, A. and Green, B.
Notes: The pGEM®-T Vector System was used in this study. (2005)
Milner, L., Bigas, A., Kopan, R., Brashem-Stein, C., Bernstein, I. and Martin, D.
Notes: The pGEM®-T Vector System was used in this study. (1970)
Yang, Y. and Klessig, D.F.
Notes: The pGEM®-T Vector System was used to clone PCR products amplified from a tobacco cDNA library. (1968)
Farci, P., Shimoda, A., Wong, D., Cabezon, T., DeGioannis, D., Strazzera, A., Shimizu, Y., Shapiro, M., Alter, H., Purcell, R.
Notes: The pGEM®-T Vector System was used in this study. (2193)
Chen, C., Wieland, T., Simon, M.
Notes: The pGEM®-T Vector System was used in this study. (1967)
Vikkula, M. , Boon, L. M. , Carraway, K. L. , Calvert, J. T. , Diamonti, A. J. , Goumnerov, B. , Pasyk, K. A. , Marchuk, D. A. , Warman, M. L. , Cantley, L. C. , Mulliken, J. B. , Olsen, B. R.
Notes: A 3,375 bp fragment was cloned in the pGEM®-T Vector System. (0223)
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