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J. Neurosci. 17, 765-773. Consequences of nigrostriatal denervation of the functioning of the basal ganglia in human and nonhuman primates: An in situ hybridization study of cytochrome oxidase subunit I mRNA. 1997

Vila, M., Levy, R., Herrero, M.T., Ruberg, M., Faucheux, B., Obeso, J.A., Agid, Y. and Hirsch, E.C.

Notes: A portion of the cytochrome oxidase subunit I cDNA was amplified and cloned into the pGEM®-T Vector. RNA probes were produced from the pGEM®-T Vector for in situ hybridization studies. (1547)

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J. Neurosci. 17, 6094-6104.. Detection of functional nicotinic receptors blocked by alpha-bungarotoxin on PC12 cells and dependence of their expression on post-translational events. 1997

Blumenthal, E.M., Conroy, W.G., Romano, S.J., Kassner, P. D. and Berg, D. K.

Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM®-T Vector and sequenced. (1419)

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J. Clin. Invest. 99(5), 838-845. Differential expression of Exons 1a and 1c in mRNAs for sterol regulatory element binding Protein-1 in human and mouse organs and cultured cells. 1997

Shimomura, I., Shimano, H., Horton, J., Goldstein, J. and Brown, M.

Notes: The pGEM®-T Vector System was used in this study. (2009)

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Proc. Natl. Acad. Sci. USA 94, 2563-2568. EEN encodes for a member of a new family of proteins containing an Src homology 3 domain and is the third gene located on chromosome 19p13 that fuses to MLL in human leukemia. 1997

So, C.W., Caldas, C., Liu, M.M., Chen, S.J., Huang, Q.H., Gu, L.J., Sham, M.H., Wiedemann, L.M. and Chan, L.C.

Notes: Promega's pGEM®-T Vector System and TNT® Coupled Reticulocyte Lysate System were used in this study. (1948)

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J. Neurosci. 17, 9145-9156. Estradiol Enhances Prostaglandin E2 Receptor Gene Expression in Luteinizing Hormone-Releasing Hormone (LHRH) Neurons and Facilitates the LHRH Response to PGE2 by Activating a Glia-to-Neuron Signaling Pathway 1997

Rage, F., Lee, B.J., Ma, Y. J., Ojeda, S. R.

Notes: Taq DNA Polymerase was used in RT-PCR reactions and the products examined by Southern hybridization. Promising bands were cut from the agarose gel and cloned with the pGEM®-T Vector System. (0529)

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Proc. Natl. Acad. Sci. USA 94, 4211-4216. Expression of a borage desaturase cDNA containing an N-terminal cytochrome b5 domain results in the accumulation of high levels of delta6- desaturated fatty acids in transgenic tobacco. 1997

Sayanova, O., Smith, M.A., Lapinskas, P., Stobart, A.K., Dobson, G., Christie, W.W., Shewry, P.R., Napier, J.A.

Notes: The authors use the Reverse Transcription System, Wizard® PCR Preps DNA Purification Systems, Wizard® Plus Minipreps DNA Purification System and the pGEM®-T Vector System in their studies. (0445)

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J. Neurosci. 17, 6678-6684. Expression of zinc transporter gene, ZnT-1, is induced after transient forebrain ischemia in the gerbil. 1997

Tsuda, M., Imaizumi, K., Katayama, T., Kitagawa, K., Wanaka, A., Tohyama, M., Takagi, T.

Notes: PCR products generated by differential display analysis were subcloned into the pGEM®-T Vector System and subsequently sequenced. (0240)

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Proc. Natl. Acad. Sci. USA 94, 2415-2420. Floral homeotic genes were recruited from homologous MADS-box genes preexisting in the common ancestor of ferns and seed plants. 1997

Munster, T., Pahnke, J., Di Rosa, A., Kim, J.T., Martin, W., Saedler, H., Theissen, G.

Notes: The 3'-RACE products were cloned with the pGEM®-T Vector System. (0673)

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Am. J. Physiol. 273, C1937-C1946. Functional and molecular characterization of NHE3 expression during ontogeny in rat jejunal epithelium. 1997

Collins, J.F., Xu, H., Kiela, P.R., Zeng, J., Ghishan, F.K.

Notes: The pTargeT™ Vector was used to subclone the 2.5kb Na+/H+ Exchanger 2 and the 2.7kb Na+/H+ Exchanger 3 directly from PCR reactions. The constructs were stably transfected into PS120 Chinese hamster lung fibroblasts and selected for resistance to the neomycin analog, G-418. After 7-10 days of selection, the cells were further tested for expression by RT-PCR and immunoblotting. (1318)

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J. Mol. Neurosci. 8, 13-18. Identification of a rat brain gene associated with aging by PCR differential display method. 1997

Wu, H.C. and Lee, E.H.Y.

Notes: The pGEM®-T Vector System was used to clone the products from PCR differential display. Sequencing of the insert was performed. (1550)

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Neuron 18, 153-166. Identification of functionally distinct isoforms of the n-type Ca2+ channel in rat sympathetic ganglia and brain. 1997

Lin, Z., Haus, S., Edgerton, J. and Lipscombel, D.

Notes: The pGEM®-T Vector System was used in this study. (1969)

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Am. J. Physiol. 273, L1090-L1095. Induction of aquaporin 3 by corticosteroid in a human airway epithelial cell line. 1997

Tanaka, M., Inase, N., Fushimi, K., Ishibashi, K., Ichioka, M., Sasaki, S. and Marumo, F.

Notes: A 318bp promoter region from genomic DNA was amplified by PCR and ligated into the pGEM®-T Vector. This promoter region was then cloned into the pGL2-Basic Vector, and activity was measured using the Luciferase Assay System. Transfection efficiency was monitored with the β-Galactosidase Enzyme Assay System. (0300)

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J. Exp. Biol. 185, 1005-1012. Interleukin-18 (interferon-gamma-inducing factor) is produced by osteoblasts and acts via granulocyte/macrophage colony-stimulating factor and not via interferon-gamma to inhibit osteoclast formation. 1997

Udagawa, N., Horwood, N.J., Elliott, J., Mackay, A., Owens, J., Okamura, H., Kurimoto, M., Chambers, T.J., Martin, T.J., Gillespie, M.T.

Notes: PCR products were cloned into pGEM®-T Vector System. (0250)

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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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J. Biol. Chem. 272(14), 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to vrulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P. and Docampo, R.

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

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Proc. Natl. Acad. Sci. USA 94, 713-718. Molecular characterization of two mammalian bHLH-PAS domain proteins selectively expressed in the central nervous system. 1997

Zhou, Y.D., Barnard, M., Tian, H., Li, X., Ring, H.Z., Francke, U., Shelton, J., Richardson, J., Russell, D.W. and McKnight, S.L.

Notes: PCR products of 372bp and 426bp were subcloned into the pGEM®-T Vector and used to produce RNA probes for Northern blotting and in situ hybridization. (1552)

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J. Biol. Chem. 272, 30329-30333. Molecular cloning of a new aquaporin from rat pancreas and liver. 1997

Koyama, Y., Yamamoto, T., Kondo, D., Funaki, H., Yaoita, E., Kawasaki ,K., Sato, N., Hatakeyama, K. and Kihara, I.

Notes: The AQP8 protein was expressed in vitro using the TNT® Coupled Reticulocyte Lysate System in the presence of Canine Microsomal Membranes. The protein demonstrated an increase in molecular weight in the presence of the membranes. Promega's pGEM®-T Vector System and Proteinase K were also used. (1624)

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J. Biol. Chem. 272, 18842-18848. Molecular cloning of a novel polypeptide, DP5, induced during programmed neuronal death. 1997

Imaizumi, K., Tsuda, M., Imai, Y., Wanaka, A., Takagi, T., Tohyama, M.

Notes: Superior cervical ganglia were maintained in culture with or without the 2.5S NGF. RNA was isolated from these cultures and used for differential display with RT-PCR. The RT-PCR products were cloned with the pGEM®-T Vector System. (0998)

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Proc. Natl. Acad. Sci. USA 94, 5273-5277. Molecular cloning, sequence, expression, and processing of the interleukin 16 precursor. 1997

Baier, M., Bannert, N., Werner, A., Lang, K. and Kurth, R.

Notes: To identify the true transcriptional start of the IL-16 mRNA methods for rapid amplification of cDNA ends were applied. The complete pro-IL-16 cDNA was cloned, sequenced, and expressed in COS-7 cells. The authors used the pGEM®-T Vector System to clone fragments that were then used as templates in the Riboprobe® in Vitro Transcription System. (1453)

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Am. J. Hum. Genet. 61, 1044-1052. Mutation detection in the repeated part of the PKD1 gene. 1997

Roelfsema, J.H., Spruit, L., Saris, J.J., Chang, P., Pirson, Y., van Ommen, G.J., Peters, D.J., Breuning, M.H.

Notes: Point mutations in the repeated portion of the PKD1 gene were detected by the Protein Truncation Test. PCR products containing the T7 promoter were used as templates and protein was labeled by incorporation of [3H] leucine in a transcription/translation reaction using TNT® Coupled Reticulocyte Lysate System. The pGEM®-T Vector System was also used. (0489)

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J. Biol. Chem. 272, 5936-5942. Nerve growth factor up-regulates the N-methyl-D-aspartate receptor subunit 1 promoter in PC12 cells. 1997

Bai, G. and Kusiak, J.W.

Notes: Reporter vectors (produced with the pGL2 Basic Vector) were transiently transfected into PC12 cells and promoter activity was measured in response to 2.5S NGF. Promoter constructs were generated by PCR, subcloned into the pGEM®-T Vector and sequenced. The pGEM®-luc Vector was used to generated an antisense luc RNA probe used in RNase protection assays (using RNase ONE™ Ribonuclease) to follow the transcription of luciferase DNA following NGF treatment. The early growth reaction transcription factor and variants were expressed in the TNT® SP6 Coupled Reticulocyte Lysate System and used to perform gel shifts with promoter elements. The SP1 Consensus Oligonucleotide was used to compete transcription factor binding to the promoter elements. (1493)

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J. Neurosci. 17, 8156-8168. Nervous system-specific expression of a novel serine protease: Regulation in the adult rat spinal cord by excitotoxic injury 1997

Scarisbrick, I.A., Towner, M.D., Isackson, P.J.

Notes: PolyA+ RNA was isolated from rat total RNA using the PolyATtract® mRNA Isolation System and used for Northern analysis. The pGEM®-T Vector System was used to clone products from RT-PCR. (0447)

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J. Biol. Chem. 272(26), 16637-16643. Novel progesterone target genes identified by an improved differential display technique suggest that progestin-induced growth inhibition of breast cancer cells coincides with enhancement of differentiation. 1997

Kester, H.A., van der Leede, B.M., van der Saag, P.T. and van der Burg, B.

Notes: Poly(A+) RNA was isolated from total RNA extracted from T47D mammary carcinoma cell line using the PolyATtract® mRNA Isolation System. The RNA was used for northern blots. RQ1 was used to digest genomic DNA away from total RNA. The pGEM®-T Vector was used to subclone PCR products generated by differential display. (1673)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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J. Biol. Chem. 272, 23880-23886. Rat brain p64H1, expression of a new member of the p64 chloride channel protein family in endoplasmic reticulum. 1997

Duncan, R.R., Westwood, P.K., Boyd, A. and Ashley, R.H.

Notes: The pCI Mammalian Expression Vector was used to express the p64H1 protein in 293 cells and HT-4, a clonal neuronal cell line. Inserts in the vectors were verified for expression using the TNT® Coupled Reticulocyte Lysate System. The proteins were translated in the presence of Canine Pancreatic Microsomal Membranes (CMMs). The pGEM®-T Vector System was used to clone the PCR product of circle rapid amplification of cDNA ends to find the true 5'-end of the p64H1 cDNA.The Tfx™-20 Reagent was used to transfect 293 cells and HT-4 neuronal cells with the p64H1-expressing pCI-based plasmid. (1206)

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