Blumenthal, E.M., Conroy, W.G., Romano, S.J., Kassner, P. D. and Berg, D. K.
Notes: NGF was used to induce PC12 cell differentiation 3-5 days prior to assay. PCR amplimers representing various subunits of the acetylcholine receptor were subcloned into the pGEM®-T Vector. The resulting clones were used to produce RNA probes for RNase protection assays. Also, a chimeric a5/7-HT3 receptor was generated by PCR, subcloned into the pGEM®-T Vector and sequenced. (1419)