Mendoza, N., Phillips, G.L., Silva, J., Schwall, R., and Wickramasinghe, D.
Notes: In this paper, cell proliferation assays were performed on 22Rv1 cells (human prostate tumor cells) using the CellTiter-Glo™ Luminescent Cell Viability Assay. Cells were plated at the appropriate density (10,000 cells/well) in a 24-well multiwell plate. Twenty-four hours after plating, cells were exposed to various concentrations of CHS 828 for 24 h. DMSO in PBS was used as a solvent control. After treatment, the medium was removed and replaced with 200 μl of drug-free medium, and cells were incubated further for 48 h. For detection of the luminescent signal, CellTiter-Glo™ reagent was added, and light was measured in the Wallac 1420 Victor, a multilabel, multitask plate counter. (2606)