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Cancer Res. 62(15), 4206–4211. Action of a novel anticancer agent, CHS 828, on mouse fibroblasts: increased sensitivity of cells lacking poly (ADP-Ribose) polymerase-1. 2002

Lovborg, H., Wojciechowski, J., Larsson, R., Wesierska-Gadek, J.

Notes: Immortalized mouse embroyonic fibroblasts (MEFs) from PARP-1 -/- and PARP-1 +/+ mice were exposed to a novel anticancer drug, named CHS 828. The cytotoxic effect of increasing concentrations of CHS 828 was assayed with the the CellTiter-Glo® Luminescent Cell Viability Assay. For these experiments the researchers incubated the cells in CHS 828 containing media for 25 hours before assessment. (2860)

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Antimicrob. Agents Chemother. 46(7), 2292-2298. Comparative in vitro sensitivities of human immune cell lines, vaginal and cervical epithelial cell lines, and primary cells to candidate microbicides nonoxynol 9, C31G, and sodium dodecyl sulfate. 2002

Krebs, F.C., Miller, S.R., Catalone, B.J., Fichorova, R., Anderson, D., Malamud, D., Howett, M.K., and Wigdahl, B.

Notes: In this paper, cytotoxicity results obtained using an MTT-based assay were confirmed using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay and the CellTiter-Glo™ Luminescent Cell Viability Assay on primary human vaginal keratinocyte cultures and immortalized VK2/E6E7 cells. (2605)

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J. Neurosci. Res. 69(6), 869-879. Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity. 2002

Kanemura, Y., Mori, H., Kobayashi, S., Islam, O., Kodama, E., Yamamoto, A., Nakanishi, Y., Arita, N., Yamasaki, M., Okano, H., Hara, M. and Miyake, J.

Notes: This study explores the utility of indirect cell viability measurements based on metabolic activity for monitoring neural stem/progenitor cells. The tetrazolium salt WTS-8 and the CellTiter-Glo® Luminescent Cell Viability Assay were compared to BrdU incorporation for their ability to monitor cell proliferation. The authors conclude that both WTS-8 and CellTiter-Glo® can accurately monitor cell proliferation, but that the increased sensitivity and ease-of-use of CellTiter-Glo® assay made it an ideal choice for this application. (3123)

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Cancer Res. 62, 5485-5488. Inhibition of ligand-mediated HER2 activation in androgen-independent prostate cancer. 2002

Mendoza, N., Phillips, G.L., Silva, J., Schwall, R., and Wickramasinghe, D.

Notes: In this paper, cell proliferation assays were performed on 22Rv1 cells (human prostate tumor cells) using the CellTiter-Glo™ Luminescent Cell Viability Assay.  Cells were plated at the appropriate density (10,000 cells/well) in a 24-well multiwell plate. Twenty-four hours after plating, cells were exposed to various concentrations of CHS 828 for 24 h. DMSO in PBS was used as a solvent control. After treatment, the medium was removed and replaced with 200 μl of drug-free medium, and cells were incubated further for 48 h. For detection of the luminescent signal, CellTiter-Glo™ reagent was added, and light was measured in the Wallac 1420 Victor, a multilabel, multitask plate counter. (2606)

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