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Promega Corporation

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FEBS Lett. 555, 390-396. Hsp105 but not Hsp70 family proteins suppress the aggregation of heat-denatured protein in the presence of ADP. 2003

Yamagishi, N., Ishihara, K., Saito, Y., and Hatayama, T.

Notes: The pGL3-Control Vector was co-transfected into COS-7 cells with mammalian expression vectors expressing either heat shock protein Hsp70 or Hsp105α.  The cells were ATP-depleted in glucose-free media with 2μM rotenone and 5mM 2-deoxyglucose for up to 3 hours. Afterwards the cells were lysed with the Cell Culture Lysis Reagent and assayed for luciferase activity uaing the Luciferase Assay System. ATP depletion was verified during 2μM rotenone and 5mM 2-deoxyglucose treatment using the CellTiter-Glo® Assay.  (2841)

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Assay Drug Dev. Technol. 3, 469-477. HTS Compatible Assay for Antioxidative Agents Using Primary Cultured Hepatocytes 2003

Gaunitz, F. and Heise, K.

Notes: Primary hepatocytes from Sprauge-Dawley rats were incubated with various concentrations of tert-butylhydroperoxide to induce oxidative stress and then cell viability, cytotoxicity and apoptosis were assayed with Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay, CellTiter-Blue Cell Viability Assay, CellTiter-Glo Luminescent Cell Viability Assay, CytoTox-ONE Homogeneous Membrane Integrity Assay, Apo-ONE Homogeneous Caspase-3/7 Assay and assay systems. (2969)

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Cancer Res. 63(1), 119-124. Identification of genes responsible for cell migration by a library of randomized ribozymes. 2003

Suyama, E., Kawasaki, H., Kasaoka, T., Taira, K.

Notes: Cell chemotaxis was assessed by the CellTiter-Glo® assay through rounds of directed selection. Data was presented as the migrating cells as a percent of total cells. (2913)

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J. Cell. Biochem. 89, 1222-1234. Non-apoptogenic killing of HeLa cervical carcinoma cells after short exposure to the alkylating agent N-methyl-N0-nitro-N-nitrosoguanidine (MNNG). 2003

Wesierska-Gadek, J., Gueorguieva, M., Schloffer, D., Uhl, M. and Wojciechowski, J.

Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used to assess the cytotoxic effects of N-methyl-N’-nitro-N-nitrosoguanidine (MNNG) on HeLa cells. Cells were assayed after a 3 hour treatment with 50μM MNNG. Data is presented as the relative light units observed with each sample. (2755)

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Int. J. Cancer 106, 486-495. Rapid onset of nucleolar disintegration preceding cell cycle arrest in roscovitine-induced apoptosis of human MCF-7 breast cancer cells. 2003

Wojciechohski, J., Horky, M., Gueorguieva, M., and Wesierska-Gadek, J.

Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used to measure viability of human breast cancer MCF-7 and MCF-7.3.28 cells after 24 hour incubation with roscovitine (ROSC).   A dose-dependent cytotoxic effect was observed on 60-70% confluent cells with increasing ROSC concentrations from 0.01 to 100μM. Data are expressed as percent viable cells compared to untreated controls. (2840)

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Nat. Immunol. 4(9), 874-881. The threshold pattern of calcineurin-dependent gene expression is altered by loss of the endogenous inhibitor calcipressin. 2003

Ryeom, S., Greenwald, R.J., Sharpe, A.H. and McKeon, F.

Notes: The role of calcineurin in linking calcium signaling to transcriptional activation was explored in this study. To do this, T-cell activation in calcipressin knockout mice was investigated.  Transcriptionally activated cells were generated by incubating 1 x 105 CD4+ T-cells with plate-bound anti-CD3 or anti-CD28.  The viability of these cells was measured daily using the CellTiter-Glo® Luminescent Cell Viability Assay. Activated cells were stained with either anti-Fas ligand and anti-CD4 antibodies or with anti-IFN-γ antibody and allophycocyanin-conjugated IL4, and analyzed by flow cytometry.  (2724)

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J. Pharmacol. Exp. Ther. 303(1), 412-23. A cell-based reporter gene assay for determining induction of CYP3A4 in a high-volume system. 2002

Raucy, J., Warfe, L., Yueh, M.F., Allen, S.W.

Notes: The CellTiter-Glo® Luminescent Cell Viability Assay was used to assess the viability of HepG2 cells stably expressing CYP3A4 response elements under low or no serum conditions (10-0% FBS). (3145)

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Cancer Res. 62(15), 4206–4211. Action of a novel anticancer agent, CHS 828, on mouse fibroblasts: increased sensitivity of cells lacking poly (ADP-Ribose) polymerase-1. 2002

Lovborg, H., Wojciechowski, J., Larsson, R., Wesierska-Gadek, J.

Notes: Immortalized mouse embroyonic fibroblasts (MEFs) from PARP-1 -/- and PARP-1 +/+ mice were exposed to a novel anticancer drug, named CHS 828. The cytotoxic effect of increasing concentrations of CHS 828 was assayed with the the CellTiter-Glo® Luminescent Cell Viability Assay. For these experiments the researchers incubated the cells in CHS 828 containing media for 25 hours before assessment. (2860)

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Antimicrob. Agents Chemother. 46(7), 2292-2298. Comparative in vitro sensitivities of human immune cell lines, vaginal and cervical epithelial cell lines, and primary cells to candidate microbicides nonoxynol 9, C31G, and sodium dodecyl sulfate. 2002

Krebs, F.C., Miller, S.R., Catalone, B.J., Fichorova, R., Anderson, D., Malamud, D., Howett, M.K., and Wigdahl, B.

Notes: In this paper, cytotoxicity results obtained using an MTT-based assay were confirmed using the CellTiter-96® AQueous Non-Radioactive Cell Proliferation Assay and the CellTiter-Glo™ Luminescent Cell Viability Assay on primary human vaginal keratinocyte cultures and immortalized VK2/E6E7 cells. (2605)

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J. Neurosci. Res. 69(6), 869-879. Evaluation of in vitro proliferative activity of human fetal neural stem/progenitor cells using indirect measurements of viable cells based on cellular metabolic activity. 2002

Kanemura, Y., Mori, H., Kobayashi, S., Islam, O., Kodama, E., Yamamoto, A., Nakanishi, Y., Arita, N., Yamasaki, M., Okano, H., Hara, M. and Miyake, J.

Notes: This study explores the utility of indirect cell viability measurements based on metabolic activity for monitoring neural stem/progenitor cells. The tetrazolium salt WTS-8 and the CellTiter-Glo® Luminescent Cell Viability Assay were compared to BrdU incorporation for their ability to monitor cell proliferation. The authors conclude that both WTS-8 and CellTiter-Glo® can accurately monitor cell proliferation, but that the increased sensitivity and ease-of-use of CellTiter-Glo® assay made it an ideal choice for this application. (3123)

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Cancer Res. 62, 5485-5488. Inhibition of ligand-mediated HER2 activation in androgen-independent prostate cancer. 2002

Mendoza, N., Phillips, G.L., Silva, J., Schwall, R., and Wickramasinghe, D.

Notes: In this paper, cell proliferation assays were performed on 22Rv1 cells (human prostate tumor cells) using the CellTiter-Glo™ Luminescent Cell Viability Assay.  Cells were plated at the appropriate density (10,000 cells/well) in a 24-well multiwell plate. Twenty-four hours after plating, cells were exposed to various concentrations of CHS 828 for 24 h. DMSO in PBS was used as a solvent control. After treatment, the medium was removed and replaced with 200 μl of drug-free medium, and cells were incubated further for 48 h. For detection of the luminescent signal, CellTiter-Glo™ reagent was added, and light was measured in the Wallac 1420 Victor, a multilabel, multitask plate counter. (2606)

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