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J. Biol. Chem. 272, 12692-12698.. Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein. 1997

Chatterjee, N. , Zou, C. , Osterman, J. C. , Gupta, N. K.

Notes: Luciferase studies were performed in KRC-7 rat hepatoma cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-galactosidase Vector. Luciferase activities determined with the Luciferase Assay System were normalized to readings from the Beta-Galactosidase Enzyme Assay System. (1364)

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J. Biol. Chem. 272, 6573-6577. Cloning of the human phospholipase C-gamma1 promoter and identification of a DR6-type vitamin D-responsive element. 1997

Xie, Z. , Bikle, D. D.

Notes: Expression of luciferase reporter constructs, derived from the pGL3 Vectors, was studied in human keratinocytes. (0166)

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J. Biol. Chem. 272, 20994-2097. Complex regulation of the BRCA1 gene 1997

Xu, C. F. , Chambers, J. A. , Solomon, E.

Notes: Expression of luciferase reporter constructs, derived from pGL3 Basic Vectors, was studied in MCF7, T47D, BT20, SKOV3 and JAR human cancer cell lines. The β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer was also used. (0121)

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J. Biol. Chem. 272, 7445-7454. Constitutive and beta-naphthoflavone-induced expression of the human gamma-glutamylcysteine synthetase heavy subunit gene is regulated by a distal antioxidant response element/TRE sequence. 1997

Mulcahy, R.T., Wartman, M.A., Bailey, H.H., Gipp, J.J.

Notes: Reporter studies were performed in HepG2 cells. The experimental constructs were made using the the pGL3 Basic Vector and luciferase activities were monitored with the Luciferase Assay System. (0669)

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J. Biol. Chem. 272, 25176-25183.. Genomic organization of human and mouse genes for vascular endothelial growth factor C. 1997

Chilov, D. , Kukk, E. , Taira, S. , Jeltsch, M. , Kaukonen, J. , Palotie, A. , Joukov, V. , Alitalo, K.

Notes: Luciferase studies were performed in HeLa cells using constructs prepared in the pGL3 Basic Vector. (1339)

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Proc. Natl. Acad. Sci. USA 94, 2687-2692. Glucocorticoid receptor pathways are involved in the inhibition of astrocyte proliferation. 1997

Crossin, K.L., Tai, M.H., Krushel, L.A., Mauro, V.P. and Edelman, G.M.

Notes: Studies performed in primary astrocytes. (1613)

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Am. J. Physiol. 273, G833-G841. Hepatocyte nuclear factor-1α regulates transcription of the guanylin gene. 1997

Hochman, J.A., Sciaky, D., Whitaker, T.L., Hawkins, J.A., Cohen, M.B.

Notes: Authors make 3 base changes in a promoter element using the Altered Sites® II in vitro Mutagenesis System and put the mutated promoter into the pGL3-Basic Vector. Luciferase activity was measured using the Luciferase Assay System. (1047)

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Gene Ther. 4, 162-171. High efficiency reporter gene transfection of vascular tissue in vitro and in vivo using a cationic lipid-DNA complex. 1997

Keogh, M.C., Chen, D., Lupu, F., Shaper, N., Schmitt, J.F., Kakkar, V.V., Lemoine, N.R.

Notes: The authors used Tfx™-50 Reagent to transfect or microinject pGL3-Basic Vector constructs into rabbit, rat and human arterial smooth muscle cells and HepG2 cell lines.  For the rabbit carotid arteries, this was an in vivo transfer of DNA. (0930)

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EMBO J. 16, 5943-5954.. High mobility group chromosomal protein 1 binds to the adeno-associated virus replication protein (Rep) and promotes Rep-mediated site-specific cleavage of DNA, ATPase activity and transcriptional repression 1997

Costello, E. , Saudan, P. , Winocour, E. , Pizer, L. , Beard, P.

Notes: Luciferase studies were performed in 293T cells using constructs prepared in the pGL3 Basic Vector. (1278)

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J. Biol. Chem. 272, 16431-16437. Identification and characterization of multiple osmotic response sequences in the human aldose reductase gene. 1997

Ko, B.C.B., Ruepp, B., Bohren, K.M., Gabbay, K.H., Chung, S.S.M.

Notes: Chang liver cells were transfected with constructs in pGL3-Basic and pGL3-Promoter Vectors using the Tfx™-50 Reagent. Constructs were verified with the fmol® DNA Cycle Sequencing System. (0911)

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J. Biol. Chem. 272, 30583-30588. Identification of a Placental Enhancer for the Human Leptin Gene 1997

Bi, S., Gavrilova, O., Gong, D.W., Mason, M.M. and Reitman, M.

Notes: Promoter activity was assayed in JEG-3 choriocarcinoma cells, JAR choriocarcinoma cells, HeLa cells and primary adipocytes. The pGL3 Vectors were transfected at a 20:1 ratio over pRL-CMV Vector. Enhancer activity was functional only in the choriocarcinoma cells. The Dual-Luciferase® Reporter Assay System was used to measure luciferase expression. (1446)

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J. Biol. Chem. 272, 27957-27965. Identification of an upstream enhancer within a functional promoter of the human leukemia inhibitory factor receptor gene and its alternative promoter usage 1997

Wang, Z. , Melmed, S.

Notes: Expression of luciferase reporter constructs, derived from the pGL3-Basic and pGL3-Promoter Vectors, was studied in placental JEG-3 cells, U-2 OS osteogenic sarcoma cells, JAR human choriocarcinoma cells, TC1 thyroid carcinoma cells, HeLa cells, Hep3B cells, MCF-7 cells and AtT20 cells. (0207)

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J. Biol. Chem. 272, 29821-29828. Interaction between the amino- and carboxyl-terminal regions of the rat androgen receptor modulates transcriptional activity and is influenced by nuclear receptor coactivators. 1997

Ikonen, T., Palvimo, J.J., Janne, O.A.

Notes: The authors used the Promega pSV-β-Galactosidase Control Vector, pGL3-Control and pGL3-Basic Vectors, Luciferase Assay Reagent, TNT® Coupled Wheat Germ Extract Systems and TNT® Coupled Reticulocyte Lysate Systems in their studies NF-κB. (0994)

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J. Clin. Invest. 100, 1797-1803. Interleukin 6 is autoregulated by transcriptional mechanisms in cultures of rat osteoblastic cells. 1997

Franchimont, N. , Rydziel, S. , Canalis, E.

Notes: Luciferase studies were performed in primary rat osteoblasts using both the pGL2 Basic and pGL3 Basic vectors. (1168)

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J. Biol. Chem. 272, 1308-1314. Involvement of the Sp3 transcription factor in induction of p21Cip1/WAF1 in keratinocyte differentiation. 1997

Prowse, D.M., Bolgan, L., Molnar, A., Dotto, G.P.

Notes: The authors used Promega's  pGL3 Basic Vector to study promoter activity in primary keratinocytes. (0517)

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J. Biol. Chem. 272, 15003-15010. Isolation and characterization of the human gp130 promoter. Regulation by STATS. 1997

O'Brien, C. A. , Manolagas, S. C.

Notes: Luciferase studies performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. The luciferase constructs were cotransfected with the pSV-beta-Galactosidase Control Vector to normalize for transfection efficiency. Luciferase activities were measured with the Luciferase Assay System. (0629)

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J. Biol. Chem. 272, 9742-9748. Modulation of interferon action by retinoids. Induction of murine STAT1 gene expression by retinoic acid. 1997

Weihua, X. , Kolla, V. , Kalvakolanu, D. V.

Notes: Expression of luciferase reporter constructs, derived from the pGL3-Basic Vector, was studied in COS-7 cells. (0177)

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J. Biol. Chem. 272, 20055-20062. Oxygen-regulated Transferrin Expression Is Mediated by Hypoxia- inducible Factor-1 1997

Rolfs, A., Kvietikova, I., Gassmann, M., Wenger, R.H.

Notes: Luciferase studies were performed on extracts of transfected Hep3B, HepG2 and HeLa cells. The experimental constructs were prepared in the pGL3 Basic and pGL3 Promoter Vectors. (0452)

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Mol. Pharmacol. 51, 620-629. Promoter analysis of the rat β1-adrenergic receptor gene identifies sequences involved in basal expression. 1997

Bahouth, S.W., Cui, X., Beauchamp, M.J., Shimomura, H., George, S.T. and Park, E.A.

Notes: Reporter studies were performed in primary rat ventricular myocytes, HepG2 cells and SK-N-MC cells using the pGL3 Basic Vector. All luciferase values were normalized to β-galactosidase activity provided by the pSV-β-Galactosidase Control Vector. PCR-generated deletion mutants of promoter constructs were subcloned into the pGEM®-T Vector and sequenced. (1491)

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J. Biol. Chem. 272, 17802-17809. PU.1 Is Essential for p47(phox) Promoter Activity in Myeloid Cells. 1997

Li, S.L., Valente, A.J., Zhao, S.J., Clark, R.A.

Notes: Luciferase studies were performed in HL-60 cells. Experimental constructs were prepared in pGL3 Basic Vector and luciferase activities determined with the Luciferase Assay System. (0796)

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Clin. Exp. Hypertens. 19, 543-550. Regulation of human renin gene transcription by cAMP. 1997

Germain, S., Konoshita, T., Fuchs, S., Philippe, J., Corvol, P. and Pinet, F.

Notes: Authors performed luciferase assays using the pGL3 Basic Vector in Calu-6 cells. (1151)

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J. Clin. Invest. 99, 2906-2914. Regulation of the Rat Liver Sodium-dependent Bile Acid Cotransporter Gene by Prolactin:  Mediation of Transcriptional Activation by Stat5. 1997

Ganguly, T.C. , O'Brien, M.L. , Karpen, S.J. , Hyde, J.F. , Suchy, F.J. , Vore, M.

Notes: Luciferase studies were performed in HepG2 cells using constructs prepared in the pGL3 Basic Vector. (1137)

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J. Biol. Chem. 272, 31755-31763. Signal transduction-mediated activation of the aryl hydrocarbon receptor in rat hepatoma H4IIE cells. 1997

Backlund, M., Johansson, I., Mkrtchian, S., Ingelman-Sundberg, M.

Notes: Studies were performed in H4IIE rat hepatoma cells. The pGL3-based Vectors were co-transfected with the Renilla control vector (pRL-SV40 Vector) at a 6:1 ratio and assayed using the Dual-Luciferase® Reporter Assay System. (1489)

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J. Biol. Chem. 272, 20691-20697.. Signaling from G protein-coupled receptors to the c-jun promoter involves the MEF2 transcription factor. Evidence for a novel c-jun amino-terminal kinase-independent pathway 1997

Coso, O. A. , Montaner, S. , Fromm, C. , Lacal, J. C. , Prywes, R. , Teramoto, H. , Gutkind, J. S.

Notes: Luciferase studies were performed in NIH3T3 cells expressing 20,000 muscarinic acetylcholine receptors per cell using constructs prepared in the pGL3 Promoter Vector. (1276)

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Mol. Pharmacol. 51, 250-261. Structural and functional characterization of the human alpha3 nicotinic subunit gene promoter. 1997

Fornasari, D., Battaglioli, E., Flora, A., Terzano, S. and Clementi, F.

Notes: Studies were performed in the neuroblastoma cells lines SY5Y and SK-N-BE and the human rhabdomyosarcoma cell line TE671. All reporter activities were reported as fold-stimulation over pGL3 Basic Vector basal expression. (1570)

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