Krüttgen, A., Möller, J.C., Heymach, J.V. Jr, and Shooter, E.M.
Notes: To examine the regulation of neurotrophin secretion, the release of neurotrophins from PC12 cells transfected with various neurotrophin expression vectors was quantitated. To quantitate BDNF an ELISA was developed by coating the plates with the TrkB-Fc receptor body and detecting with the Anti-Human BDNF pAb and the Anti-Chicken IgY, HRP Conjugate. There was no crossreactivity with NGF, NT-3, and NT-4/5 at 1 µg/ml. The Anti-Human p75 pAb was also used in this study. (2359)