Hu, Y.-L., Li, S., Shyy, J.Y.-J., Chien, S.
Notes: The Apoptosis Detection System, Fluorescein, was used in triple fluorescent labeling. Bovine aortic endothelial cells were treated with colchicine and analyzed for the presence of β-Galactosidase (marker for transfection), TUNEL and nuclei (Hoechst dye). Transfection with a dominant negative mutant JNK (K-R) prevented the appearance of TUNEL staining in the cells and also eliminated the DNA ladder characteristic of apoptosis. The name of the Apoptosis Detection System, Fluorescein, has been changed to DeadEnd™ Fluorometric TUNEL System. (1025)