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Appl. Environ. Microbiol. 64, 669-677. Reverse transcriptase (RT) inhibition of PCR at low concentrations of template and its implications for quantitative RT-PCR. 1998

Chandler, D. P. , Wagnon, C. A. , Bolton, H. Jr

Notes: This paper discusses the inhibition of PCR by reverse transcriptases. The inhibitory effect was overcome by increasing template concentration to more than 10^5 to 10^6 copies or including T4 gene 32 protein during the reverse transcription phase of the reaction. The poly(A)+ RNA used in the study was isolated using the PolyATtract® mRNA Isolation System. (1357)

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Biochem. J. 332, 773-780. Transcript heterogeneity of the human reduced folate carrier results from the use of multiple promoters and variable splicing of alternative upstream exons. 1998

Zhang, L. , Wong, S. C. , Matherly, L. H.

Notes: The authors used the PolyATtract® mRNA Isolation System to isolate mRNA from total RNA. They used this mRNA-rich fraction for primer extension analysis using Promega's AMV Reverse Transcriptase. The Wizard® Plus Midiprep DNA Purification System was used for various plasmid isolations and the Erase-a-Base®  System was used to generate deletion series. The Dual-Luciferase® Reporter Assay System was used to study promoters cloned into the pGL3-Basic Vector. The pRL-SV40 was used as a transfection control plasmid. (0095)

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EMBO J. 16(13), 3944-3954. A new gene encoding a putative transcription factor regulated by the Drosophila circadian clock. 1997

Rouyer, F., Rachidi, M., Pikielny, C. and Rosbash, M.

Notes: Poly A+ RNA was isolated from Drosophila head total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for the production of subtraction cDNA libraries. (1685)

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J. Biol. Chem. 272(39), 24480-24487. A novel family of small cysteine-rich antimicrobial peptides from seed of Impatiens balsamina is derived from a single precursor protein. 1997

Tailor, R.H., Acland, D.P., Attenborough, S., Cannue, B.P.A., Evans, I.J., Osborn, R.W., Ray, J.A., Rees, S.B. and Broekaert, W.F.

Notes: PolyA+ RNA was isolated from I. balsamina seed total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA synthesis. (1689)

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Proc. Natl. Acad. Sci. USA 94, 9746-9750. A screen for fast evolving genes from Drosophila 1997

Schmid, K. J., Tautz, D.

Notes: PolyA+ RNA was isolated from Drosophila yakuba embryo total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (0410)

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Proc. Natl. Acad. Sci. USA 94(18), 9746-9750. A screen for fast evolving genes from Drosophila. 1997

Schmid, K.J. and Tautz, D.

Notes: PolyA+ RNA was isolated from Drosophila yakuba embryo total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (1687)

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J. Biol. Chem. 272(3), 1565-1570. A structurally novel transferrin-like protein accumulates in the plasma membrane of the unicellular green alga Dunaliella salina grown in high salinities. 1997

Fisher, M., Gokhman, I., Pick, U. and Zamir, A.

Notes: Poly A+ RNA was isolated from D. salina total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. (1722)

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EMBO J. 16(21), 318-331. Abrogation of a mitotic checkpoint by E2 proteins from oncogenic human papillomaviruses correlates with increased turnover of the p53 tumor suppressor protein. 1997

Frattini, M.G., Hurst, S.D., Lim, H.B., Swaminathan, S. and Laimins, L.A.

Notes: PolyA+ RNA was isolated from secondary normal human kidney cell total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for Northern analysis. (1724)

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Proc. Natl. Acad. Sci. USA 94, 5728-5732. BCL8, a novel gene involved in translocations affecting band 15q11-13 in diffuse large-cell lymphoma. 1997

Dyomin, V.G., Rao, P.H., Dalla-Favera, R. and Chaganti, R.S.K.

Notes: In this study, total RNA was isolated from frozen tumor specimens and cell lines using the RNAgents® Total RNA Isolation System. The PolyATtract® mRNA Isolation System was used to isolate Poly(A)+ RNA from B cell lymphocytic leukemia cell total RNA. (2027)

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J. Biol. Chem. 272(30), 18538-18541. cDNA cloning of human retinoic acid-metabolizing enzyme (hP450RAI) identifies a novel family of cytochromes P450 (CYP26). 1997

White, J.A., Beckett-Jones, B., Guo, Y.-D., Dilworth, F.J., Bonasoro, J., Jones, G. and Petkovich, M.

Notes: Poly A+ RNA was isolated from the total RNA of nine different human cell lines using the PolyATtract® mRNA Isolation System. The RNA was examined by Northern analysis. (1696)

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J. Biol. Chem. 272, 5464-5468. Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae. 1997

Ranson, H., Cornel, A.J., Fournier, D., Vaughan, A., Collins, F.H. , Hemingway, J.

Notes: The PolyATtract® mRNA Isolation System IV was used to isolate poly(A)+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis using Promega's Universal Riboclone® cDNA Synthesis System. (0498)

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J. Biol. Chem. 272(9), 5464-5468. Cloning and localization of a glutathione S-transferase class I gene from Anopheles gambiae. 1997

Ranson, H., Cornel, A.J., Fournier, D., Vaughan, A., Collins, F.H. and Hemingway, J.

Notes: The PolyATtract® mRNA Isolation System was used to isolate poly A+ RNA from Anopheles gambiae (mosquito) total RNA. The isolated RNA was used for cDNA synthesis with the Universal Riboclone System. (1683)

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J. Biol. Chem. 272(30), 18702-18708. CYP26, a novel mammalian cytochrome P450, is induced by retinoic acid and defines a new family. 1997

Ray, W.J., Gain, F., Yao, M. and Gottlieb, D.I.

Notes: Poly A+ RNA was isolated from ES and 4-3+ cells. Unclear whether starting material was cells or total RNA. The isolated polyA+ RNA was used for Northern analysis. (1684)

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J. Neurosci. 17, 8817-8827. Defective learning in mutants of the drosophila gene for a regulatory subunit of cAMP-dependent protein kinase. 1997

Goodwin, S.F., Del Vecchio, M., Velinzon, K., Hogel, C., Russell, S.R.H., Tully, T., Kaiser, K.

Notes: The PolyATtract® mRNA Isolation System was used to isolate poly(A)+ RNA from total adult Drosophila RNA. The isolated RNA was used for Northern analysis. (1111)

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J. Neurosci. 17, 3136-47. Differential expression of K4-AP currents and Kv3.1 potassium channel transcripts in cortical neurons that develop distinct firing phenotypes. 1997

Massengill, J.L., Smith, M.A., Son, D. I., O'Dowd, D.K.

Notes: PCR products were directly sequenced with the fmol® DNA Cycle Sequencing System using the end-labeled primer protocol. (0739)

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J. Biol. Chem. 272, 16364-16373.. Expression of mixed lineage kinase-1 in pancreatic beta-cell lines at different stages of maturation and during embryonic pancreas development 1997

DeAizpurua, H. J. , Cram, D. S. , Naselli, G. , Devereux, L. , Dorow, D. S.

Notes: Poly(A)+ RNA was isolated from RIN rat insulinoma cell total RNA with PolyATtract® mRNA Isolation System III. (1264)

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J. Biol. Chem. 272(26), 16364-16373. Expression of mixed lineage kinase-1 in pancreatic beta-cell lines at different stages of maturation and during embryonic pancreas development. 1997

DeAixpurua, H.J., Cram, D.S., Naselli, G., Devereux, L. and Dorow, D.S.

Notes: Poly(A+) RNA was isolated from RIN rat insulinoma cell total RNA and was used for Northern blots. The authors used Promega's PolyATtract® mRNA Isolation System and AMV Reverse Transcriptase in this study. (2107)

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Blood 89(11), 4058-4062. Genomic sequence and transcription start site for the human γ-glutamyl carboxylase. 1997

Wu, S.M., Stafford, D.W., Frazier, L.D., Fu, Y.Y., High, K.A., Chu, K., Sanchez-Vega, B. and Solera, J.

Notes: The PolyATtract® mRNA Isolation System was used to isolate polyA+ RNA from total human liver RNA. The isolated RNA was used for primer extension analysis. (1697)

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Blood 89, 4058-4062. Genomic sequence and transcription start site for the human gamma- glutamyl carboxylase. 1997

Wu, S. M. , Stafford, D. W. , Frazier, L. D. , Fu, Y. Y. , High, K. A. , Chu, K. , Sanchez-Vega, B. , Solera, J.

Notes: The PolyATtract® mRNA Isolation System II was used to isolate polyA+ RNA from total human liver RNA. The isolated RNA was used for primer extension analysis. (0159)

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J. Biol. Chem. 272, 7306-7313. Heat shock inhibits release of the signal recognition particle from the endoplasmic reticulum in barley aleurone layers. 1997

Chu, B. , Brodl, M. R. , Belanger, F. C.

Notes: The PolyATtract® mRNA Isolation System was used to isolate poly(A)+ RNA from barley total RNA. The isolated RNA was used for Northern analysis. (1299)

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Blood 89, 1896-1904. Human platelets display high-affinity receptors for thrombopoietin. 1997

Broudy, V.C., Lin, N.L., Sabath, D.F., Papayannopoulou, T. and Kaushansky, K.

Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)

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J. Exp. Med. 185, 453-459. Identification of tyrosinase-related protein 2 as a tumor rejection antigen for the B16 melanoma. 1997

Bloom, M.B., Perry-Lalley, D., Robbins, P.F., Li, Y., el-Gamil, M., Rosenberg, S.A. and Yang, J.C.

Notes: Poly(A) RNA was isolated using the PolyATtract® mRNA Isolation System and used to create a library in a mammalian expression vector. Pools of 25 colonies of E.coli clones were used to transfect 293 cells in a limited dilution screen. (2044)

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J. Biol. Chem. 272, 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to virulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P., Docampo, R.

Notes: PolyATtract® mRNA Isolation System was used to isolate Poly A+ RNA from the protozoan Leishmania mexicanna amazonesis total RNA. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM®-T Vector. (0748)

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J. Biol. Chem. 272(14), 9464-9473. Intracellular Ca2+ pool content and signaling and expression of a calcium pump are linked to vrulence in Leishmania mexicana amazonesis amastigotes. 1997

Lu, H.G., Zhong, L., Chang, K.P. and Docampo, R.

Notes: Poly A+ RNA was isolated from the protozoan Leishmania mexicanna amazonesis total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for RT-PCR. The RT-PCR products were cloned into the pGEM-T Vector. (1678)

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J. Biol. Chem. 272(41), 25678-25684. Isolation from phage display libraries of single chain variable fragment antibodies that recognize conformational epitopes in the malaria vaccine candidate, apical membrane antigen-1. 1997

Fu, Y., Shearing, L.N., Haynes, S., Crewther, P., Tilley, L., Anders, R.F. and Foley, M.

Notes: The PolyATtract® mRNA Isolation System was used to isolate polyA+ RNA from mouse spleen total RNA. The isolated RNA was used for cDNA synthesis. (1725)

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