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J. Neurosci. 18, 8369-8381. Autocrine hepatocyte growth factor provides a local mechanism for promoting axonal growth. 1998

Yang, X.-M., Toma, J.G., Bamji, S.X., Belliveau, D.J., Kohn, J., Park, M., Miller, F.D.

Notes: NGF-dependent pure sympathetic rat neurons from the superior cervical ganglion were cultured 2 days in various concentrations of NGF plus HGF, NGF plus anti-HGF or HGF alone. The surviving neurons were assayed with the CellTiter 96® Non-Radioactive Cell Proliferation System. The authors use 50µl of Dye Solution to 500µl of media in a 24-well plate with a reaction time of 2 hours. The cells were lysed in 100µl of a 0.065N HCl/isopropanol solution rather than the recommended Solubilization/Stop Solution. (0142)

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Infect. Immun. 66, 3198-3207. Bioluminescence as a reporter of intracellular survival of Bordetella bronchiseptica in murine phagocytes 1998

Forder, C.B., Parton, R., Coote, J.G.

Notes: Murine macrophage cells (P388D1)  were pretreated with recombinant gamma interferon, cytochalasin D or mondansylcadaverine prior to infection with Bordetella bronchispetica and viability of the infected macrophages was determined using CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2540)

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Blood 91, 1548-1555. Both stroma and stem cell factor maintain long-term growth of ELM erythroleukemia cells, but only stroma prevents erythroid differentiation in response to erythropoietin and interleukin-3 1998

O'Prey, J., Leslie, N., Itoh, K., Ostertag, W., Bartholomew, C., Harrison, P.R.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to assess the growth factor requirements of murine erythroleukemia cells in culture. The cells were cultured for five days with various cytokines and growth factors prior to assay. Several of the IGF-1 independent clones analyzed by western blotting with the Anti-ACTIVE® MAPK pAb were found to have a constitutive level of activated MAP Kinase. Study also shows serum is a very potent activator of MAPK in serum-starved cells. (0567)

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Blood 91(5), 1548-55. Both stroma and stem cell factor maintain long-term growth of ELM erythroleukemia cells, but only stroma prevents erythroid differentiation in response to erythropoietin and interleukin-3. 1998

O'Prey, J., Leslie, N., Itoh, K., Ostertag, W., Bartholomew, C. and Harrison, P. R.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to assess the effects of IGF-I, insulin or Stem Cell Factor (SCF) on growth of murine erythroleukemia cells. The effects of neutralizing antibodies against the growth factors were also tested. Promega’s Anti-ACTIVE® MAPK pAb, with specificity for the active, dually phosphorylated form of MAPK was used for Western blotting to assay activation of mitogen-activated protein (MAP) kinases. Several of the IGF-1 independent clones analyzed by western blotting and found to have a constitutive level of activated MAP Kinase. Serum was shown to be a potent activator of MAPK in serum-starved cells. (2080)

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Cell Death Differ. 5, 271-288. Cell death attentuation by 'usurpin', a mammalian DED-caspase homologue that precludes caspase-8 recruitment and activation by the CD-95 (Fas, APO-1) receptor complex 1998

Rasper, D.M., Vaillancourt, J.P., Hadano, S., Houtzager, V.M., Seiden, I., Keen, S.L.C., Tawa, P., Xanthoudakis, S., Nasir, J., Martindale, D., Koop, B.F., Peterson, E.P., Thornberry, N.A., Huang, J., MacPherson, D.P., Black, S.C., Hornung, F., Lenardo, M.J., Hayden M.R. , Roy, S., Nicholson, D.W.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to monitor the number of viable HeLa cells following exposure to an Anti-Fas antibody. HeLa cells stably transfected with usurpin lacking the DED domain were viable at higher doses of the anti-fas antibody than control cells. (0500)

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Cell Death Differ. 5, 440-449. Cripto: Roles in mammary cell growth, survival, differentiation and transformation 1998

Niemeyer, C.C., Persico, M.G., Adamson, E.D.

Notes: The CellTiter® 96 Non-Radioactive Cell Proliferation Assay was used to monitor proliferation of CID-9 mouse mammary epithelial cells transfected with a Cripto-1 expressing vector. (0612)

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Antimicrob. Agents Chemother. 42, 1713-1717. Effect of trovafloxacin on production of cytokines by human monocytes. 1998

Khan, A.A., Slifer, T.R., Remington, J.S.

Notes: The broad-spectrum fluoroquinoline antibiotic, travafloxacin, was shown to suppress synthesis of various cytokines from cultured human monocytes. The CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) was used to determine the decrease in synthesis was not due to the toxic effect of the antibiotic on the monocytes. (0934)

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Cell Death Differ. 5(1), 96-106. Egr-1 inhibits apoptosis during the UV response: Correlation of cell survival with Egr-1 phosphorylation. 1998

Huang, R-P., Fan, Y., deBelle, I., Ni, Z., Matheny, W. and Adamson, E.D.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to measure the effect of kinase inhibitors on the growth of NIH3T3 cells 2 days after UV irradiation. Kinase inhibitors significantly reduced UV-induced phosphorylation of Egr-1, transactivating ability and cell growth. (2088)

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J. Biol. Chem. 273, 12584-12592. Endothelin-induced apoptosis of A375 human melanoma cells. 1998

Okazawa, M., Shiraki, T., Ninomiya, H., Kobayashi, S., Masaki, T.

Notes: The CellTiter 96® Non-radioactive Cell Proliferation assay was used to monitor the response of asynchronous and phase synchronized A375 cells to increasing amounts of endothelin-1. (0601)

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J. Cell Biol. 140, 431-446. Glycosylation provides both stimulatory and inhibitory effects on cell surface and soluble CD44 binding to hyaluronan 1998

Skelton, T.P., Zeng, C., Nocks, A., Stamenkovic, I.

Notes: Ldl-D or CHO cells were incubated with an anti-CD44 mAb or a isotype-matched control antibody and plated onto hyaluronan coated plates. Nonadherent cells were washed away and adherent cells quantified with the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT). Percentage adherent was determined by comparing washed wells to unwashed wells. (0374)

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Am. J. Physiol. 275, G556-G563. Induction of endothelin-1 synthesis by IL-2 and its modulation of rat intestinal epithelial cell growth. 1998

Shigematsu, T., Miura, S., Hirokawa, M., Hokari, R., Higuchi, H., Watanabe, N., Tsuzuki, Y., Kimura, H., Tada, S., Nakatsumi, R.C., Saito, H., Ishii, H.

Notes: The authors used the CellTiter 96® Non Radioactive Cell Proliferation Assay to study cell proliferation of IEC-6 and IEC-18 cells (rat intestinal epithelia). (0394)

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Antimicrob. Agents Chemother. 42, 3179-3186. Inhibition of Human Hepatitis B Virus Replication by AT-61, a phenyl propenamide derivative, alone and in combination with (-)β-L-2', 3'-dideoxy-3'-thiacytidine 1998

King, R.W., Ladner, S.K., Miller, T.J., Zaifert, K., Perni, R.B., Conway, S.C., Otto, M.J.

Notes: Cytotoxicity of AT-61 for HepG2 cells an HEPAD38 cells (a HepG2 cell loine that produces HBV when grown in the absence of tetracycline) was assessed using the Cell Titer 96® Non-Radioactive Cell Proliferation Assay. To assay for the inhibition of the tetracycline promoter activity in HepAD43 cells (produces β-galactosidase in the absence of tetracycline), β-galactosidase activity was determined using the Reporter Lysis Buffer from the β-Galactosidase Assay System. (2506)

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J. Biol. Chem. 273, 28633-28641. Laminin-1 and Laminin-2 G-domain synthetic peptides bind syndecan-1 and are involved in acinar formation of a human submandibular gland cell line 1998

Hofman, M.P., Nomizy, M., Roque, E., Lee, S., Jung, D.W., Yamada, Y., Kleinman, H.K.

Notes: HSG cells were cultured with laminin-derived peptides for 48 hours.  Cell proliferation was measured using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2507)

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J. Immunol. 161, 3711-3718. Membrane cofactor protein: Importance of N- and O-glycosylation for complement regulatory function 1998

Liszewski, M.K., Leung, M.K., Atkinson, J.P.

Notes: Membrane co-factor protein (MCP) inhibits complement activation on host cells. Chinese Hamster Ovary cells were transfected with wildtype, reverse orientation, and mutant MCP lacking various domains. Cells bearing equivalent expression levels of the transfection product were tested in cytoprotection assays in a classical C pathway-mediated complement system using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. The authors present data as (2484)

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J. Neurosci. 18, 9790-9799. Overexpression in neurons of human presenilin-1 or a presenilin-1 Familial Alzheimer Disease mutant does not enhance apoptosis 1998

Bursztajn, S., DeSouza, R., McPhie, D.L., Berman, S.A., Shioi, J., Robakis, N.K., Neve, R.L.

Notes: Primary cortical neurons isolated from day 18 mice were isolated and infected with Herpes Simplex Virus vectors expressing either the wildtype human presenilin-1 gene or a clinically significant mutant of the presenilin-1 gene. Cell viability assays of the infected cells were performed using the CellTiter 96® Non-Radioactive Cell Proliferation Assay to determine whether cell survival was affected. (2536)

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Mol. Cell. Biol. 18, 1236-1247. Overexpression of the nucleoporin CAN/NUP214 induces growth arrest, nucleocytoplasmic transport defects and apoptosis 1998

Boer, J., Bonten-Surtel, J. and Grosveld, G.

Notes: The CAN gene product was expressed from a tetracycline inducible promoter in U937T cells. Induction of the protein inhibited cell growth, and uninduced cells were indistinguishable from untransfected control cells. Co-expression of Bcl-XL could not rescue the cells. Cell proliferation was monitored every day for four days using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. Inhibition of cell proliferation was directly proportional to the amount of the CAN gene product expressed. (1420)

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J. Immunol. 161, 4356-4365. Oxidized α2-Macroglobulin (α2-M) differentially regulates receptor binding by cytokines/growth factors: Implications for tissue injury and repair mechanisms in inflammation 1998

Wu, S.M., Patel, D.D., Pizzo, S.V.

Notes: TNF-α cytotoxicity assays on WEHI-13VAR cells were carried out using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2553)

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J. Cell Biol. 143, 1691-1703. P53 is essential for developmental neuron death as regulated by the TrkA and p75 neurotrophin receptors 1998

Aloyz, R.S., Bamji, S.X., Pozniak, C.D., Toma, J.G., Atwal, J., Kaplan, D.R., Miller, F.D.

Notes: Rat sympathetic neurons from the superior cervical ganglion of postnatal day 1 rats were used for survival assays in NGF-withdrawal experiments and p75 activation experiments. Cell survival was determined using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2535)

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Mol. Cell. Biol. 18, 3245-3256. RACK 1, a receptor for activated C kinase and a homolog of the β subunit of G proteins, inhibits activity of Src tyrosine kinases and growth of NIH 3T3 cells 1998

Chang, B.Y., Conroy, K.B., Machleder, E.M., Cartwright, C.A.

Notes: The authors used a TnT® Coupled Reticulocyte Lysate System to transcribe and translate Src in vitro. Cell proliferation of NIH 3T3 cells that stably expressed either HA-RACK or vector only was assessed using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2537)

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J. Immunol. 160, 5098-5104. Regulation of TNF-α Production in Activated Mouse Macrophages by Progesterone 1998

Miller, L. and Hunt, J.S.

Notes: The authors used the CellTiter 96® Non-Radioactive Cell Proliferation Assay to determine cell viability of mouse macrophage-like cells prior to all experiments measuring gene expression and TNF-α production. (2478)

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J. Virol. 72, 2752-2759. RNase L mediates the antiviral effect of interferon through a selective reduction in viral RNA during encephalomyocarditis virus infection. 1998

Li, X.-L., Blackford, J.A., Hassel, B.A.

Notes: NIH 3T3 cells with inducible expression of RNase L were treated with IFN-a/b then expression of RNase L was induced. The cells were then challenged with encephalomyocarditis virus and vesicular stomatitis virus. Viable cells 18hr after virus challenge were determined by use of the CellTiter 96® Proliferation Assay (MTT). The interferon/RNase L induction only protected the cells from the encephalomyocarditis virus. (0800)

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Blood 92, 1989-2002. Saturation mutagenesis of the β Subunit of the human granulocyte-macrophage colony-stimulating factor receptor shows clustering of constitutive mutations, activation of ERK MAP Kinase and STAT pathways and differential β subunit tyrosine phosphorylation 1998

Jenkins, B.J., Blake, T.J., Gonda, T.J.

Notes: The authors performed a concerted mutagenesis and constructed point-mutated hβc libraries (hβc is the common signal transducing β subunit of GM-CSF, IL-3, and IL-5 receptors).  Mouse pro-B BAF-B03 cells and mouse myeloid FDC-P1 cells were transiently transfected with high-titer retroviruses carrying the mutant hβc cDNAs.  Lines expressing different mutated cDNAs were assayed for mGM-CSF- or mIL3- dependent and independent cell proliferation using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (2479)

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Mol. Cell. Biol. 18, 2089-2099. SHP-1 binds and negatively modulates the c-Kit receptor by interaction with tyrosine 569 in the c-Kit juxtamembrane domain. 1998

Kozlowski, M., Larose, L., Lee, F., Le, D.M., Rottapel, R., Siminovitch, K.A.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to monitor the proliferation of untransfected Ba/F3 and c-Kit-expressing Ba/F3 pro-B-cells in response to IL-3. No significant difference was observed over 8 days. (0887)

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Antimicrob. Agents Chemother. 42, 339-343. Synthesis and evaluation of dinitroanilines for treatment of cryptosporidiosis. 1998

Benbow, J.W., Bernberg, E.L., Korda, A. and Mead, J.R.

Notes: Various synthetic dinitroanilines were synthesized and evaluated for their potential to inhibit the infection of MDCK Madin-Darby canine kidney cells by Cryptosporidium parvum. The toxic effects of the synthesized dinitroanilines on the MDCK cells was evaluated with the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT). (1437)

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J. Cell Biol. 140, 911-923. The p75 neurotrophin receptor mediates neuronal apoptosis and is essential for naturally occurring sympathetic neuron death. 1998

Bamji, S. X., Majdan, M., Pozniak, C. D., Belliveau, D. J., Aloyz, R., Kohn, J., Causing, C. G. and Miller, F. D.

Notes: The MTT-based CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to assess the survival of primary sympathetic neurons from the rat superior cervical ganglion. The effects of NT-4, NGF, BDNF, interaction with the p75 neurotropin receptor were investigated. (1540)

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