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J. Bacteriol. 180, 2043-2049. ntn genes determining the early steps in the divergent catabolism of 4-nitrotoluene and toluene in Pseudomonas sp. Strain TW3. 1998

James, K.D., Williams, P.A.

Notes: The Access RT-PCR System was used to study expression of three ntn genes from TW3 cells grown in either 4-nitrotoluene, toluene or succinate. The pET-5a Vector (discontinued) was used to express the ntnC protein in E. coli strain BL21(DE3) pLysS. (0968)

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Biochemistry 37(15), 5173-5183. Single amino acid substitutions at the N-terminus of a recombinant cytotoxic ribonuclease markedly influence biochemical and biological properties. 1998

Newton, D.L., Boque, L., Wlodawer, A., Huang, C.Y. and Rybak, S.M.

Notes: Naturally isolated onconase, a cellular RNase with anti-tumor properties, is not inhibited by the placental ribonuclease inhibitor. The natural protein has a pyroglutamate at the N-terminus as the result of a posttranslational modification starting from an N-terminal methionine followed by a glutamate residue. Recombinant onconase has no activity unless the N-terminal methionine and the glutamate residue is cyclized to form the pyroglutamate moiety. However, conversion of the glutamate to either serine or tyrosine results in RNase activity without processing. This RNase activity is very different from the natural onconase because the Ser and Tyr mutants are inhibited by RNasin® Ribonuclease Inhibitor. Further studies determined that the Tyr and Ser mutants display a 100,000-fold greater sensitivity to the inhibitor that the natural onconase. (1646)

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J. Immunol. 161, 474-480. System administration of endotoxin induces bronchopulmonary hyperactivity dissociated from TNF-alpha formation and neutrophil sequestration into the murine lungs. 1998

Lefort, J., Singer, M., Leduc, D., Renesto, P., Nahori, M.A., Huerre, M., Créminon, Chignard, M., Vargaftig, B.B.

Notes: Poly A+ RNA was isolated directly from PBS-perfused mouse lungs with the PolyATtract® System 1000. The isolate poly A+ RNA was used for quantitative two-step RT-PCR using M-MLV RNase Hminus Reverse Transcriptase as well as RNasin® Ribonuclease inhibitor for first step cDNA synthesis. (0821)

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Genetics 150, 643-650. Tetrahymena mutants with short telomeres. 1998

Ahmed, S., Sheng, H., Niu, L. and Henderson, E.

Notes: PCR products were excised, eluted, and precipitated prior to sequencing with the fmol® DNA Cycle Sequencing System. Promega's RNasin® Ribonuclease Inhibitor and AMV Reverse Transcriptase were used in a reverse transcription assay. (2065)

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J. Biol. Chem. 273, 5607-5614. Transcriptional activation of the UDP-glucuronosyltransferase 1A7 gene in rat liver by aryl hydrocarbon receptor ligands and oltipraz. 1998

Metz, R.P. and Ritter, J.K

Notes: The RNasin Ribonuclease Inhibitor was used to protect RNA transcripts during primer extension analysis and in vitro transcription of RNA probes. The resultant RNA probes were used for RNase protection assays. (1639)

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Am. J. Pathol. 153, 381-394. Vascular endothelial growth factor-C (VEGF-C/VEGF-2) promotes angiogenesis in the setting of tissue ischemia. 1998

Witzenbichler, B., Asahara, T., Murohara, T., Silver, M., Spyridopoulos, I., Magner, M., Principe, N., Kearney, M., Hu, J.-S., Isner, J.M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to monitor the proliferative response of human umbilical vein endothelial cells to VEGF-C. RT-PCR was performed in the presence of RNasin® Ribonuclease Inhibitor. (0146)

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Cell 90(2), 303-311. A human endogenous retroviral superantigen as candidate autoimmune gene in type I diabetes. 1997

Conrad, B., Weissmahr, R.N., Böni, J., Arcari, R., Schüpbach, J. and Mach, B.

Notes: The inhibitor was used to protect RNA during RT-PCR. (1671)

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J. Biol. Chem. 272(33), 20756-20763. Characterization of point mutations in patients with X-linked ichthyosis: Effects on the structure and function of the steroid sulfatase protein. 1997

Alperin, E.S. and Shapiro, L.J.

Notes: The ribonuclease inhibitor was used to protect RNA during the reverse transcriptase reaction for RT-PCR. The RNasin® Ribonuclease Inhibitor is also used in the RiboMAX® System for RNA stabilization during in vitro transcription. Transcripts were produce with the RiboMAX® System and expresed in Rabbit Lysate with or without Canine Microsomal Membranes. Proteinase K protection assays are performed with proteins expressed in the presence of the membranes with and without Triton X-100 solubilization of the membranes. The proteinase K digests were stopped by the addition of PMSF and immediately loading the sample into 100°C SDS Sample buffer. (1670)

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Proc. Natl. Acad. Sci. USA 94(7), 3206-3210. Cloning and characterization of the extreme 5'-terminal sequences of the RNA genomes of GB virus C/hepatitis G virus. 1997

Hsieh, S. Y., Yang, P. Y., Chen, H. C., and Liaw, Y. F.

Notes: The RNasin® Ribonuclease Inhibitor and the pGEM®-T Vector System were used in this study. The inhibitor was used to protect RNA during the T4 RNA ligase step for 5' RACE analysis. (1643)

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Blood 89, 2422-2428. HPA-10w(b) (La(a)): genetic determination of a new platelet-specific alloantigen on glycoprotein IIIa and its expression in COS-7 cells. 1997

Peyruchaud, O., Bourre, F., Morel-Kopp, M. C., Reviron, D., Mercier, P., Nurden, A., Kaplan, C.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to remove an EcoR I site for greater ease in subcloning and to introduce a mutation in gpIIIa found in a patient. The mutations were confirmed by sequencing. The mutants were subcloned into a mammalian expression vector and transfected into COS-7 cells. Many details of the transfection are provided. Promega's Taq DNA Polymerase, fmol® DNA Cycle Sequencing System, Altered Sites® in vitro Mutagenesis System and Transfectam® Reagent were used in this study. (0562)

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J. Cell Biol. 138, 5-16. Human cytomegalovirus immediate early interaction with host nuclear structures: definition of an immediate transcript environment. 1997

Ishov, A.M., Stenberg, R.M. and Maul, G.G.

Notes: The authors used RNasin® Ribonuclease Inhibitor during DNase treatment of cells. (1006)

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Blood 89, 1896-1904. Human platelets display high-affinity receptors for thrombopoietin. 1997

Broudy, V.C., Lin, N.L., Sabath, D.F., Papayannopoulou, T. and Kaushansky, K.

Notes: In this study, Poly(A)+ RNA was isolated from B16 melanoma cell total RNA using the PolyATtract® mRNA Isolation System. The isolated RNA was used for cDNA library construction. The RNAgents® Total RNA Isolation System was used to isolate total RNA from HEL and K562 human erythroleukemia cells. The isolated RNA was used for RT-PCR. RNasin® Ribonuclease Inhibitor was used in a very innovative fashion. Peripheral mononuclear cells isolated from blood were directly lysed in a hypotonic buffer containing the inhibitor. The lysates from these cells were used directly for RT-PCR. (2026)

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J. Cell Biol. 136(5), 1059-1070. Isoforms of ankyrin-3 that lack the NH2-terminal repeats associate with mouse macrophage lysosomes. 1997

Hoock, T.C., Peters, L.L. and Lux, S.E.

Notes: The inhibitor was used to protect RNA during RT-PCR. (2246)

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Genes Dev. 11, 3168-3181. Mediation of NGF signaling by post-translational inhibition of HES-1, a basic helix-loop-helix repressor of neuronal differentiation. 1997

Strom, A., Castella, P., Rockwood, J., Wagner, J., Caudy, M.

Notes: NGF was used in this study of neurite outgrowth in PC12 cells under a variety of conditions. Protein Kinase C was used to in vitro phosphorylate a bacterially expressed protein and the phospho protein was assayed by gel shift. Many details of the phosphorylation are provided. (0304)

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Cell 88(3), 385-392. Mutation of the Ca2+ channel b subunit gene Cchb4 is associated with ataxia and seizures in the lethargic (lh) mouse. 1997

Burgess, D.L., Jones, J.M., Meisler, M.H. and Noebels, J.L.

Notes: Poly(A)+ RNA was isolated from mouse brain total RNA and used for Northern analysis. The authors used Promega's PolyATtract® mRNA Isolation System, AMV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor in this study. (2104)

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J. Mol. Neurosci. 8, 29-44. On the role of the low-affinity neurotrophin receptor p75LNTR in nerve growth factor induction of differentiation and AP1 binding activity in PC12 cells. 1997

Kontny, E., Ciruela, F., Svenningsson, P., Ibáñez, C.F. and Fredholm, B.B.

Notes: The inhibitor was used to protect RNA during RT-PCR. 125I-NGF was produced and used to crosslink to cell surface proteins on PC12 cells. The NGF was also used to assay for neurite outgrowth of PC12 and two clonal variants. Cells were treated with NGF and nuclear extracts made and used to assay for AP1 binding activity. Tyrosine phosphorylation of PI kinase in response to NGF was assayed. Also, the concentration-dependent effect of NGF on the accumulation of 3H-inositol phosphate was examined in the PC12 cells. The bFGF was used to assay the induction of cell differentiation in the PC12 cell and a clonal variant. (1632)

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EMBO J. 16, 5784-5795. Plasmid rolling circle replication: identification of the RNA polymerase-directed primer RNA and requirement for DNA polymerase I for lagging strand synthesis. 1997

Kramer, M.G., Khan, S.A. , Espinosa, M.

Notes: The Altered Sites® II in vitro Mutagenesis System was used to create seven different mutant ssoA genes. The mutations also incorporated new restriction sites for easy screening. (0891)

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EMBO J. 16, 4361-4373. Remodeling of regulatory nucleoprotein complexes on the Xenopus hsp70 promoter during meiotic maturation of the Xenopus oocyte. 1997

Landsberger, N. and Wolffe, A.P.

Notes: The inhibitor was used to protect transcripts during a run-on transcription assay. (1638)

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J. Neurosci. 17, 6974-6987. Selective expression of insulin-like growth factor II in the songbird brain. 1997

Holzenberger, M., Jarvis, E.D., Chong, C., Grossman, M., Nottebohm, F. and Scharff, C.

Notes: T7 RNA Polymerase and SP6 RNA Polymerase were used to produce RNA probes labeled with digoxygenin-11-UTP. Transcripts were made in the presence of RNasin® Ribonuclease Inhibitor in a standard transcription reaction. The ribonucleotides ATP, CTP and GTP were used at 1mM. Both UTP and digoxygenin-11-UTP were used at 0.5mM in the reaction. The RNA probe was used for in situ hybridization. (1563)

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J. Exp. Med. 185, 695-706. The Immunodominant Antigen of an Ultraviolet-induced Regressor Tumor Is Generated by a Somatic Point Mutation in the DEAD Box Helicase p68. 1997

Dubey, P., Hendrickson, R., Meredith, S., Siegel, C., Shabanowitz, J., Skipper, J., Engelhard, V., Hunt, D., Schreiber, H.

Notes: Promega's Taq DNA Polymerase, RNasin® Ribonuclease Inhibitor, and fmol® DNA cycle Sequencing System were used in this study. (1952)

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J. Biol. Chem. 272, 20936-20944. The p38/Reactivating kinase mitogen-activated protein kinase cascade mediates the activation of the transcription factor insulin upstream factor 1 and insulin gene transcription by high glucose in pancreatic beta-cells. 1997

Macfarlane, W.M., Smith, S.B., James, R.F.L., Clifton, A.D., Doza, Y.N., Cohen, P., Docherty, K.

Notes: The AMV Reverse Transcriptase, RNasin® Ribonuclease Inhibitor and Taq DNA Polymerase were used for RT-PCR to isolate a clone of the human insulin upstream factor 1, a transcription factor. The protein was expressed in vitro with the Rabbit Reticulocyte Lysate and used for gel shift assays. Reporter studies were performed in MIN6 cells. (0762)

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Nucl. Acids Res. 24(5), 970-976. A binding factor for interleukin 2 mRNA. 1996

Hua, J. and Paetkau, V.

Notes: Jurkat cells, a human T lymphocyte line that can be induced to synthesize and secrete interleukin 2, contain a factor that binds interleukin 2 mRNA. The binding is sequence specific and occurs in the 3’-non-coding region, within 160nt of the end of the coding region, at or near a site on the mRNA that is rich in A and U residues. However, it appears not to be due to known AU binding factors. The factor is protease sensitive and binds non-covalently to interleukin 2 mRNA. It behaves like aprotein of molecular weight 50,000-60,000 after UV-induced cross-linking to the mRNA. Preparations of the binding factor also protect interleukin 2 mRNA against degradation by a recently described RNasin-resistant endoribonuclease activity in Jurkat cells. Protection occurs under the same conditions required to generate the gel-retarded complex. (1645)

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J. Clin. Invest. 97(7), 1761-1766. CD40 expression by human peripheral blood eosinophils. 1996

Ohkawara, Y., Xing, K., Glibetic, M., Nakano, K., Dolovich, J., Croitorus, K., Weller, P. and Jordana, M.

Notes: These authors used Promega's AMV Reverse Transcriptase, RNasin® Ribonuclease Inhibitor and Taq DNA Polymerase in this study. (2061)

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Immunity 5, 253-262. Cytotoxic T Cell-Resistant Variants Are Selected in a Virus-Induced Demyelinating Disease. 1996

Pewe, L., Wu, G., Barnett, E., Castro, R., Perlman, S.

Notes: Promega's Taq DNA Polymerase, fmol® DNA Cycle Sequencing System and RNasin® Ribonuclease Inhibitor were used in this study. (1954)

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Proc. Natl. Acad. Sci. USA 93, 15180-15184. Estrogen-induced transcription of the progesterone receptor gene does not parallel estrogen receptor occupancy. 1996

Lee, Y. and Gorski, J.

Notes: Promega's Taq DNA Polymerase, RNasin® Ribonuclease Inhibitor and M-MLV Reverse Transcriptase were used in this study. (2003)

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