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Wong, D. J. , Barrett, M. T. , Stoger, R. , Emond, M. J. , Reid, B. J.
Notes: DNA with unmethylated cytosines converted to uracil by bisulfite treatment was purified with the Wizard® PCR Preps DNA Purification System. The DNA was used for methylation-specific PCR. (0149)
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Wizard® PCR Preps DNA Purification System
Mitsuda, N., Roses, A.D. and Vitek, M.P.
Notes: The Dual-Luciferase™ Reporter System was used to quantitate the presenilin promoter activity in Neuro2a neuroblastoma cells, mouse P19 embryonal carcinoma cells and NIH 3T3 cells. Studies were also performed in P19 cells treated with retinoic acid to acquire a neuron-like phenotype and P19 cells treated with dimethyl sulfoxide to acquire a muscle-like phenotype. The presenilin promoter functioned best in the Neuro2a and neuron-like P19 cells. 5´-RACE products from mouse brain RNA were purified with the Wizard® PCR Preps System and cloned into the pGEM-T Vector . The cloned amplimers were sequenced and used as a template for amplification to produce truncation mutants to assess promoter activity. (1590)
Dual-Luciferase® Reporter Assay System
pGEM®-T Vector System I
pGEM®-T Vector System II
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Arystarkhova, E., Sweadner, K.J.
Notes: The authors used the RNAgents® Total RNA Isolation System to isolate RNA from rat cardiac myocytes. They also used the Wizard® PCR Preps DNA Purification System to isolate DNA from agarose. (2230)
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