Fabretti, F., Theilacker, C., Baldassarri, L., Kaczynski, Z., Kropec, A., Holst, O. and Huebner, J.
Notes: This study investigated the effect of teichoic acid alanylation on biofilm formation, adhesion, sensitivity to antimicrobial peptides and resistance to neutrophil killing in Enterococcus faecalis. The dlt operon in E. faecalis controls D-alanylation of teichoic acids. A deletion mutant lacking a portion of the dltA gene, which encodes a putative D-alanine activating enzyme, was created. Compared to the wildtype strain, the E. faecalis dltA deletion mutant produced less biofilm, exhibited reduced adhesion to cultured epithelial cells, was more sensitive to various antimicrobial peptides and less sensitive to opsonic killing. Plasmids used during the mutagenesis procedure and for complementation studies were purified from E. coli or Enterococci using the PureYield™ Plasmid Midipreps System or the Wizard® Plus SV Minipreps System. (3526)