We believe this site might serve you best:

United States

English Continue

This country code will remain if no action is taken to change it.

Don't see your country?
Promega Corporation
Home » Resources » Tools »

Citations Search

Catalog_banner_2014

Need Assistance? Chat

Sort By:

Eur. J. Biochem. 267, 7224–7229. Identification and characterization of a novel transcriptional regulator, MatR, for malonate metabolism in Rhizobium leguminosarum bv. trifolii 2000

Lee, H.Y., An, J.H. and Kim, Y.S.

Notes: Total RNA was isolated from Rhizobium leguminosarum bv. trifolii using the SV Total RNA Isolation System. This RNA was used in a primer extension assay using Promega's AMV Reverse Transcriptase to determine the transcriptional start site of the mat operon. Nested deletions during the sequencing of the mat promoter were prepared using the Erase-a-Base® System. The firefly luciferase gene from pGL3 Basic was used to construct a reporter construct containing the mat promoter to study transcriptional effects of MatR. The interaction of the MatR protein with the mat operator was mapped using the Core Footprinting System. (2308)

Expand Full Notes »

EMBO J. 19, 1068–1078. Intimate adhesion of Neiserria meningitidis to human epithelial cells is under the control of the crgA gene, a novel LysR-type transcriptional regulator 2000

Deghmane, A.E., Petit, S., Topilko, A., Periera, Y., Giorgini, D., Larribe, M., and Taha, M.K.

Notes: Total RNA was isolated from Neiserria meningitidis using the SV Total RNA Isolation System. The relative levels of crgA transcript in crgA mutants and wild type strains was determined by RT-PCR with the Access RT-PCR System. (2309)

Expand Full Notes »

Am. J. Pathol. 157, 1991–2002. Intracerebral recruitment and maturation of dendritic cells in the onset and progression of experimental autoimmune encephalomyelitis. 2000

Serafini, B., Columba-Cabezas, S., Di Rosa, F. and Aloisi, F.

Notes: Total RNA was isolated from the CNS, spleen and small intestine of mice with preclinical, acute, remission and relapse EAE with the SV Total RNA Isolation System. The isolated RNA was used to analyze gene expression in each stage using the Reverse Transcriptase System and Taq DNA Polymerase in two step RT-PCR. (2163)

Expand Full Notes »

J. Gen. Virol. 81, 1103–1109. Mutational evidence that the VPg is involved in the replication and not the movement of Pea enation mosaic virus-1. 2000

Skaf, J.S., Schultz, M.H., Hirata, H. and de Zoeten, G.A.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from 100mg of pea seedling leaves. The isolated RNA was used for Northern blots and RT-PCR. Full transcripts of the Pea enation mosaic virus-1 and Pea enation mosiac virus-2 were produced with T7 RNA Polymerase in vitro and translated in Rabbit Reticulocyte Lysate. (2174)

Expand Full Notes »

Proc. Natl. Acad. Sci. USA 97, 14620–14625. Pivotal role of cyclic nucleoside phosphodiesterase 4 in Tat-mediated CD4+ T cell hyperactivation and HIV type 1 replication 2000

Secchiero, P., Zella, D., Curreli, S., Mirandola, P., Capitani, S., Gallo, R.C. and Zauli, G.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from freshly isolated human CD4+ T cells. The isolated RNA was used for RT-PCR with the Access RT-PCR System. (2183)

Expand Full Notes »

Biochem. J. 346, 537–543. Rapid replenishment of sphingomyelin in the plasma membrane upon degradation by sphingomyelinase in NIH3T3 cells overexpressing the phosphatidylinositol transfer protein beta. 2000

van Tiel, C.M., Luberto, C., Snoek, G.T., Hannun, Y.A. and Wirtz, K.W.A.

Notes: The SV Total RNA Isolation System was used to isolate RNA from transfected NIH3T3 cells. The isolated RNA was used for RT-PCR. (2159)

Expand Full Notes »

J. Biol. Chem. 275, 33548–33553. Regulation by Glucocorticoids of Expression and Activity of rBSC1, the Na+-K+(NH4+)-2Cl- Cotransporter of Medullary Thick Ascending Limb 2000

Attmane-Elakeb, A., Sibella, V., Vernimmen, C., Belenfant, X./Hebert, S.C. and Bichara, M.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from rat kidney medulla and medullary thick ascending limb. The isolated RNA was used in a quantitative RT-PCR. (0073)

Expand Full Notes »

J. Cell Biol. 14, 667–682. Roles of autocrine TGF-beta receptor and Smad signaling in adipocyte differentiation. 2000

Choy, L., Skillington, J. and Derynck, R.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from 3T3-F442A preadipocytes after various treatments. The isolated RNA was used for Northern analysis. (2181)

Expand Full Notes »

Am. J. Pathol. 156, 939–950. Semaphorin SEMA3F localization in malignant human lung and cell lines : A suggested role in cell adhesion and cell migration. 2000

Brambilla, E., Constantin, B., Drabkin, H. and Roche, J.

Notes: The SV Total RNA Isolation System was used to isolate RNA from various lung cancer cell lines. The isolated RNA was used for RT-PCR and quantitative RT-PCR. (2161)

Expand Full Notes »

Clin. Can. Res. 6, 3823–3826. Telomerase RNA as a detection marker in the serum of breast cancer patients 2000

Chen, X.Q., Bonnefoi, H., Pelte, M.F., Lyautey, J., Lederrey, C., Movarekhi, S., Schaeffer, P., Mulcahy, H.E., Meyer, P., Stroun, M. and Anker, P.

Notes: Total RNA was isolated from human serum by combining 100µl of serum with 175µl of SV RNA Lysis Buffer. Only fresh or once-frozen serum was used. The SV Total RNA Isolation System was also used to isolate total RNA from tumor tissue. The rest of the protocol was followed as directed in the technical manual. The quantities of RNA isolated from serum were too low to quantitate so 1µl or 5µl of the isolated RNA was used in RT-PCR to analyze RNA content. (2160)

Expand Full Notes »

Infect. Immun. 68, 3368–3376. Transriptional Organization and Function of Invasion Genes within Salmonella enterica Serovar Typhimurium Pathogenicity Island 1, Including the prgH, prgI, prgJ, prgK, orgA, orgB, and orgC Genes 2000

Klein, J.R., Fahlen, T.F., and Jones, B.D.

Notes: Total RNA was isolated from a virulent strain of Salmonella enterica, SL1344 using the SV Total RNA Isolation System. The Access RT-PCR System was used to characterize the transcriptional organization of the prg operon. The pgrH gene was amplified by RT-PCR and cloned into the pGEM®-T vector (2305)

Expand Full Notes »

J. Biol. Chem. 275, 28722–28730. TWIK-2, an inactivating 2P domain K+ channel. 2000

Patel, A.J., Maingret, F., Magnone, V., Fosset, M., Lazdunski, M. and Honore, E.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from various rat tissues. The isolated RNA was used for RT-PCR. The authors also use a derivative of the pCI Vector called pCI-IRES-CD8 to express the TWIK protein specifically in COS cells. (2179)

Expand Full Notes »

J. Biol. Chem. 275, 7176–7183. Vear, a novel golgi-associated protein with VHS and gamma-adaptin 'ear' domains 2000

Poussu, A., Lohi, O. and Lehto, V.P.

Notes: The SV Total RNA isolation System was used to isolate total RNA from HeLa human cervical carcinoma cells, COS-7 SV40-transformed monkey kidney cells, MDBK bovine kidney cells and MDCK canine kidney cells. The isolated RNA was used for Northern blot analysis. (0543)

Expand Full Notes »

J. Bacteriol. 181, 4734–4740. An Unspliced Group I Intron in rRNA Links Chlamydiales, Chloroplasts and Mitochondria 1999

Everett, K.D., Kahane, S., Bush, R.M. and Friedman, M.G.

Notes: Total RNA was isolated from uninfected Vero cells and Vero cells infected with either Simkania negevensis ZT or Chlamydia trachmatis using the SV Total RNA Isolation System. The isolated RNA was used in RT-PCR amplifications to determine the size of the unspliced intron form of the 23S rRNA to differentiate between a group I intron where the rRNA intron is spliced with religation and an intervening segment where the rRNA is fragmented to functionally remove the intervening segments. The isolated RNAs and Promega's RNA Markers were separated by electrophoresis to judge RNA integrity. (2302)

Expand Full Notes »

Am. J. Pathol. 155, 1977–1984. Basic fibroblast growth factor synthesis by human peritoneal mesothelial cells: Induction by interleukin-1. 1999

Cronauer, M.V., Stadlmann, S., Klocker, H., Abendstein, B., Eder, I.E., Rogatsch, H., Zeimet, A.G., Marth, C. and Offner, F.A.

Notes: The SV Total RNA Isolation System was used to isolate RNA from human peritoneal mesothelial cells. The isolated RNA was used for RT-PCR. (2164)

Expand Full Notes »

J. Physiol. 519, 323–333. Cloning and functional expression of a novel degenerin-like Na+ channel gene in mammals. 1999

Sakai, H., Lingueglia, E., Champigny, G., Mattei, M.-G. and Lazdunski, M.

Notes: Taq DNA Polymerase was used extensively to clone the BLINaC cDNA. The resulting clone was put into the pCI Mammalian Expression Vector. Total RNA was isolated from rat liver and primary hepatocytes with the SV Total RNA Isolation System. The RNA was used for RT-PCR. Northern blotting was performed with poly-A(+) RNA isolated with the PolyATtract® mRNA Isolation System. (0472)

Expand Full Notes »

Nucl. Acids Res. 27, e39. Comprehensive transcript analysis in small quantities of mRNA by SAGE-lite. 1999

Peters, D.G., Kassam, A.B., Yonas, H., O'Hare, E.H., Ferrell, R.E. and Brufsky, A.M.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human cerebrovascular tissue removed during surgery. The isolated RNA was used for first strand cDNA synthesis for the SAGE procedure. (2178)

Expand Full Notes »

Clin. Diagn. Lab. Immunol. 6, 471–478. Cytokine mRNA expression in lesions in cats with chronic gingivostomatitis. 1999

Harley, R., Helps, C.R., Harbour, D.A., Gruffydd-Jones, T.J. and Day, M.J.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from gingivostomatitis tissue. The RNA was eluted from the basket with 35µl of water, which was passed through the basket three times. Eleven microliters of the eluted material was used for two-step RT-PCR. One microliter of each eluted sample was analyzed for genomic DNA contamination by PCR, and any samples that did amplify were put through the SV RNA System again. As reported by the authors, 'Genomic DNA contamination was undetectable in the vast majority of sample eluates after a single RNA extraction procedure. The remaining samples were subsequently shown to be free from detectable genomic DNA after RNA reextraction.' (1064)

Expand Full Notes »

J. Biol. Chem. 274, 37070–37078. Cytosolic chaperonin is up-regulated during cell growth: Preferential expression and binding to tubulin at G1/S transition through early S phase. 1999

Yokota, S., Yanagi, H., Yura, T. and Kubota, H.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from various mouse cells lines: DA3 IL-3 dependent myeloid cells; 7TD1 IL-6-dependent B-cell hybridoma; FM3A mammary carcinoma; L5287Y lymphoma; WEHI-3 myeloma; LLC1 Lewis lung carcinoma; L929 fibroblast; J774A macrophage-like cells. Five micrograms of each RNA was used for Northern analysis. The Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) was used to determine if HL60 were apoptotic when treated with retionic acid to induce growth arrest. Less than 10% of the cells were positive in the TUNEL assay (data not shown). (0113)

Expand Full Notes »

J. Invest. Dermatol. 113, 43–48. Enhanced expression of eotaxin and CCR3 in atopic dermatitis. 1999

Yawalker, N., Uguccioni, M., Scharer, J., Braunwalder, J., Karlen, S., Dewald, B., Braathen, L.R. and Baggiolini, M.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from human skin biopsies. Two hundred nanograms of the isolated RNA was used for RT-PCR amplification with the Access RT-PCR System. For added sensitivity, nested PCR was performed as well. (0108)

Expand Full Notes »

J. Biol. Chem. 274, 26477–26484.. Functional characterization of the promoter of the x-linked ectodermal dysplasia gene. 1999

Pengue, G., Srivastava, A.K., Kere, J., Schlessinger, D. and Durmowicz, M.C.

Notes: Total RNA was isolated from transfected HeLa cells with the SV Total RNA Isolation System and used for primer extension analysis. Reporter studies were performed in HeLa, 293 and HaCaT cell lines with constructs prepared in the pGL2 Basic and Promoter Vectors. (0006)

Expand Full Notes »

J. Bacteriol. 181, 4592–4597. Genetic Characterization of a Cell Envelope-Associated Proteinase from Lactobacillus helveticus CNRZ32. 1999

Pederson, J.A., Mileski, G.J., Weimar, B.C., and J.L. Steele

Notes: Total RNA was isolated from Lactobacillus helveticus for use in 5´ RACE (Rapid Amplification of cDNA Ends) to map the 5' end of the prtH RNA which encodes a cell envelope associated proteinase involved in casein hydrolysis. (2303)

Expand Full Notes »

Science 285, 248–251. HMG-1 as a late mediator of endotixin lethality in mice. 1999

Wang, H., Bloom, O., Zhang, M., Vishnubhakat, J.M., Ombrellino, M., Che, J., Frazier, A., Yang, H., Ivanova, S., Borovikova, L., Manogue, K.R., Faist, E., Abraham, E., Andersson, J., Andersson, U., Molina, P.E., Abumrad, N.N., Sama, A. and Tracey, K.J.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from RAW 264.7 macrophages treated for various times with endotoxin. The isolated RNA was used for RT-PCR analysis of High Mobility Group-1 gene expression as well as β-actin. RT-PCR was performed with the Access RT-PCR System. (0198)

Expand Full Notes »

Biochem. J. 341, 453–460. Metabolic characteristics of a human hepatoma cell line stably transfected with hormone-sensitive lipase 1999

Pease, R.J., Wiggins, D., Saggerson, E.D., Tree, J., Gibbons, G.F.

Notes: The SV Total RNA Isolation System was used to isolate total RNA from transfected and untransfected HepG2 cells. The isolated RNA was used for Northern blot analysis. (0554)

Expand Full Notes »

J. Bacteriol. 181, 3310–3316. NahY, a Catabolic Plasmid-Encoded Receptor Required for Chemotaxis of Pseudomonas putida to the Aromatic Hydrocarbon Naphthalene. 1999

Grimm, A.C. and Harwood, C.S.

Notes: Total RNA was isolated from Pseudomonas putida using the SV Total RNA Isolation System. RT-PCR using the Access RT-PCR System allowed the authors to characterize the transcriptional organization of several genes involved in naphthalene degradation. (2307)

Expand Full Notes »

It appears that you have Javascript disabled. Our website requires Javascript to function correctly. For the best browsing experience, please enable Javascript.

Scientists at Your Service

Scientists at Your Service

We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.

Ask us! We are here to help you.