Koh, J.Y. and Choi, D.W.
Notes: The authors found LDH release to be a simple means to assess glutamate mediated central neuronal cell injury of cortical cells (containing both neuronal and glial elements from fetal mice). They measured LDH by following NADH at 340nm (i.e., non-colorimetric) and found that LDH is extremely stable, even without serum, in culture medium when stored at 37°C. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to measure LDH release. (1746)