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Eur. J. Immunol. 23(12), 3217-23. Carrier-mediated uptake and presentation of a major histocompatibility complex class I-restricted peptide. 1993

Brander, C., Wyss-Coray, T., Mauri, D., Bettens, F. and Pichler, W.J.

Notes: The authors compared the CytoTox® 96 Non-Radioactive Cytotoxicity Assay to 51Cr and found the assays were equally sensitive and gave identical results. All cytotoxicity data shown are based on LDH release measurements. EBV-transformed B cell lines, flu peptide specific effector cells also were used (all human lines and cells). (1737)

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J. Neurosci. Methods 20(1), 83-90. Quantitative determination of glutamate mediated cortical neuronal injury in cell culture by lactate dehydrogenase efflux assay. 1987

Koh, J.Y. and Choi, D.W.

Notes: The authors found LDH release to be a simple means to assess glutamate mediated central neuronal cell injury of cortical cells (containing both neuronal and glial elements from fetal mice). They measured LDH by following NADH at 340nm (i.e., non-colorimetric) and found that LDH is extremely stable, even without serum, in culture medium when stored at 37°C. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to measure LDH release. (1746)

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