Johann, S., Blumel, G., Lipp, M., and Forster, R.
Notes: In describing their modifications for a fluorescent dye label cytotoxicity assay, the authors compared it to Promega's CytoTox® 96 Non-Radioactive Cytotoxicity Assay. With 4 hour NK activity experiments, the results (% cytoxicity) obtained by flow cytometry agreed well with the results obtained by the CytoTox 96® Assay. They also investigated extended periods of incubation, because in some domestic animal models, NK activity requires an overnight incubation for lysis to occur. For long assays (16-20 hours of co-culture), there were instances when the LDH assay did not give meaningful data. In particular, PBMLs isolated from some individual animals yielded a high proportion of porcine PBML cells that died during this overnight assay. However, certain experiments were successful (better PBML overnight survival) and good correlation was observed with the CytoTox 96® Assay and their flow cytometry assay. (1744)