Hornick, J.L., Khawli, L.A., Hu, P., Lynch, M., Anderson, P.M. and Epstein A.L.
Notes: In this study, the CytoTox® 96 Non-Radioactive Cytotoxicity Assay was used to study antibody dependent cell-mediated cytotoxicity (ADCC). ARH-77 cells, plated at 2 x 104 cells per well in a 96-well plate, were used as target cells. Antibody or fusion proteins were added to the cells at 1μg/ml and ratios of 12.5:1 to 50:1 effector:target cells were made with MNC (bone marrow mononuclear) cells or PNC (neutrophilic polymorphonuclear) leukocytes. Plates were incubated at 37°C for 4 hours, after which supernatants were analyzed for LDH release. (3014)