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J. Immunol. 162(11), 6880-6892. Human breast carcinoma patients develop clonable oncofetal antigen-specific effector and regulatory T lymphocytes. 1999

Rohrer, J.W., Barsoum, A.L., Dyess, D.L., Tucker, J.A., Coggin, JH, Jr.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay activated human T cells mediated cytotoxicity The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay thapsigargin mediated cytotoxicity. (2914)

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J. Immunol. 163(12), 6488-6493. IFN-gamma production and cytotoxicity of IL-2-activated murine NK cells are differentially regulated by MHC class I molecules. 1999

Kubota, A., Lian, R.H., Lohwasser, S., Salcedo, M., Takei, F.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay IL-2 activated murine NK cell mediated cytotoxicity. (2977)

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J. Immunol. 163(9), 4721-4727. In vivo priming by DNA injection occurs predominantly by antigen transfer. 1999

Corr, M., von, Damm, A., Lee, D.J., Tighe, H.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay IL -2 stimulated murine splenocyte mediated cytotoxicity. (2893)

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J. Biol. Chem. 274(27), 19011-19016. Involvement of cell cycle elements, cyclin-dependent kinases, pRb, and E2F x DP, in B-amyloid-induced neuronal death. 1999

Giovanni, A., Wirtz-Brugger, F., Keramaris, E., Slack, R., Park, D.S.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay amyloid beta peptide mediated cytotoxicity. (2962)

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Br. J. Pharmacol. 127(8), 1915-1921. Pharmacological characterization of ATP- and LPS-induced IL-1beta release in human monocytes. 1999

Grahames, C.B., Michel, A.D., Chessell, I.P., Humphrey, P.P.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay ATP mediated cytotoxicity. (2992)

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Brain Res. 839(2), 253-262. Sequence of neurodegeneration and accumulation of phosphorylated tau in cultured neurons after okadaic acid treatment. 1999

Kim, D., Su, J., Cotman, C.W.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay okadaic acid and calyculin mediated cytotoxicity. (2953)

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J. Immunol. 162(4), 2281-2290. The beta-glucan-binding lectin site of mouse CR3 (CD11b/CD18) and its function in generating a primed state of the receptor that mediates cytotoxic activation in response to iC3b-opsonized target cells. 1999

Xia, Y., Vetvicka, V., Yan, J., Hanikyrova, M., Mayadas, T., Ross, G.D.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay activated mouse neutrophil, macrophage and NK cell mediated cytotoxicity. (2973)

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J. Neurosci. 19, 2455-2463. The mitogen-activated protein kinase pathway mediates extrogen neuroprotection after glutamate toxicity in primary cortical neurons. 1999

Singer, C.A., Figueroa-Masot, X.A., Batchelor, R.H., Dorsa, D.M.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess LDH release from primary cortical neurons following 24hr exposure to glutamate. Various compounds including 2.5S Nerve Growth Factor were assessed for their ability to block the glutamate-induced cytotoxicity. One of the neuroprotective factors, estrogen, was also examined for induction protein tyrosine kinase activity with the the SignaTECT® Protein Tyrosine Kinase Assay System. The induction of PTK activity peaked at 1min and returned to normal within 10min. (0367)

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Proc. Natl. Acad. Sci. USA 95, 5299-5304. Mycobacterium bovis bacille Calmette-Guérin strains secreting listeriolysin of Listeria monocytogenes. 1998

Hess, J., Miko, D., Catic, A., Lehmensiek, V., Russell, D.G., Kaufmann, S.H.E.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to determine cytotoxicity of J774A.1 macrophages infected with various M. bovis bacille Calmette-Guérin constructs or L. monocytogenes. (1042)

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Blood 91(7), 2249-2258. A20 inhibits NF-kappaB activation in endothelial cells without sensitizing to tumor necrosis factor-mediated apoptosis. 1998

Ferran, C., Stroka, D.M., Badrichani, A.Z., Cooper, J.T., Wrighton, C.J., Soares, M., Grey, S.T., Bach, F.H.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay adenovirus mediated cytotoxicity. (2959)

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Science 282(5386), 121-125. An antimicrobial activity of cytolytic T cells mediated by granulysin. 1998

Stenger, S., Hanson, D.A., Teitelbaum, R., Dewan, P., Niazi, K.R., Froelich, C.J., Ganz, T., Thoma-Uszynski, S., Melian, A., Bogdan, C., Porcelli, S.A., Bloom, B.R., Krensky, A.M., Modlin, R.L.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay perforin and granulysin mediated cytotoxicity. (2941)

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Gastroenterology 114, 657-668. Autocrine regulation of epithelial permeability by hypoxia: Role for polarized release of tumor necrosis factor-alpha 1998

Taylor, C.T., Dzus, A.L., Colgan, S.P.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay System was used to monitor LDH release from T84 intestinal epithelial cells when treated with IFNgamma. (0256)

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Infect. Immun. 66(8), 3918-3924. Hemolysin-positive enteroaggregative and cell-detaching Escherichia coli strains cause oncosis of human monocyte-derived macrophages and apoptosis of murine J774 cells. 1998

Fernandez-Prada, C., Tall, B.D., Elliott, S.E., Hoover, D.L., Nataro, J.P., Venkatesan, MM.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay Eschericia and Shigella mediated cytotoxicity. (2938)

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Gene Ther. 5(1), 31-39. Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3+ CD56+ immunologic effector cells by receptor-mediated transfection with the interleukin-7 gene. 1998

Finke, S., Trojaneck, B., Lefterova, P., Csipai, M., Wagner, E., Kircheis, R., Neubauer, A., Huhn, D., Wittig, B., and Schmidt-Wolf, I.G.H.

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox® 96 Non-Radioactive Cytotoxicity Assay System. In all cases, the transfected CIK cells had greater cytotoxicity to the targets than the mock-transfected or untransfected CIK cells. An MTT reduction assay was used to demonstrate that the transfection was not toxic to the CIK cells. (1740)

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Gene Ther. 5, 31-39. Increase of proliferation rate and enhancement of antitumor cytotoxicity of expanded human CD3+CD56+ immunologic effector cells by receptor-mediated transfection with the interleukin-7 gene. 1998

Finke, S., Trojanek, B., Lefterova, P., Csipai, M., Wagner, E., Kircheis, r., Neubauer, A., Huhn,D., Wittig, B., Schmidt-Wolf, I.G.H.,

Notes: Cytokine-induced killer (CIK) cells were transfected with an IL-7 expression vector. Cell-mediated cytotoxicity of the CIK cells was tested against A-704 renal carcinoma cells, HT 144 melanoma cells and REH pre B cell leukemic cells using the CytoTox® 96 Non-Radioactive Cytotoxicity Assay. (1156)

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Am. J. Physiol. 275(4 Pt 1), G620-G628. Indomethacin increases susceptibility to injury in human gastric cells independent of PG synthesis inhibition. 1998

Kokoska, E.R., Smith, G.S., Deshpande, Y., Wolff, A.B., Miller, TA.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay indomethacin mediated cytotoxicity. (2865)

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J. Exp. Med. 187(3), 433-438. Induction of an antigen-specific, CD1-restricted cytotoxic T lymphocyte response In vivo. 1998

Lee, D.J., Abeyratne, A., Carson, D.A., Corr, M.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay IL -2, mitomycin C-treated murine T-cell mediated cytotoxicity. (2979)

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Infect. Immun. 66, 1432-1438. Mutation of invH, but not stn, reduces Salmonella-induced enteritis in cattle 1998

Watson, P.R., Galyov, E.E., Paulin, S.M., Jones, P.W., Wallis, T.S.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay System was used to monitor the Salmonella-induced lysis of bovine alveolar macrophages. (0171)

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J. Neurosci. 18, 4083-4095. Purification of a multipotent antideath activity from bovine liver and its identification as arginase: Nitric oxide-independent inhibition of neuronal apoptosis. 1998

Esch, F., Lin, K.I., Hills, A., Zaman, K., Baraban, J.M., Chatterjee, S., Rubin, L., Ash, D.E., Ratan, R.R.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to judge the protective, anti-apoptotic activity of various substances to treatment of mouse N18 neuroblastoma cells with Sindbis virus. (1176)

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Proc. Natl. Acad. Sci. USA 95(22), 13147-13152. Quantitative analysis of the immunopotency of genetically transfected dendritic cells. 1998

Timares, L., Takashima, A., Johnston, SA.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay activated mouse splenocyte mediated cytotoxicity. (2955)

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Am. J. Physiol. 275, G322-G330. Role of calcium in adaptive cytoprotection and cell injury induced by deoxycholate in human gastric cells. 1998

Kokoska, E.R., Smith, G.S., Wolff, A.B., Deshpande, Y., Rieckenberg, C.L., Banan, A., Miller, T.A.

Notes: The authors used the CytoTox 96® Non-Radioactive Cytotoxicity Assay system to study cytoprotection of of human gastric cells. (0876)

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J. Biol. Chem. 273(49), 32895-32900. Shigella-induced apoptosis is dependent on caspase-1 which binds to IpaB. 1998

Hilbi, H., Moss, J.E., Hersh, D., Chen, Y., Arondel, J., Banerjee, S., Flavell, R.A., Yuan, J., Sansonetti, P.J., Zychlinsky, A.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay Shigella mediated cytotoxicity. (2939)

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J. Neurosci. 17, 4612-4622. Calcium homeostasis and reactive oxygen species production in cells transformed by mitochondria from individuals with sporadic Alzheimer's disease. 1997

Sheehan, J. P., Swerdlow, R. H., Miller, S. W., Davis, R. E., Parks, J. K., Parker, W. D., Tuttle, J. B.

Notes: Rather than detect cytotoxicity, the CytoTox 96® Non-Radioactive Cytotoxicity Assay System was used to quantitate cell number. Cells were first reacted with a fluorescein dye to detect reactive oxygen species (ROS), then the cells were lysed and total LDH determined. The data was reported as ROS/LDH. Studies were performed in SH-SY5Y neuroblastomas depleted of their mitochondria by removing its mitochondrial DNA. These cells were fused with platelets obtained from Alzheimer's patients and disease-free controls. (0387)

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Blood 89(12), 4437-4447. Chimeric CLL-1 antibody fusion proteins containing granulocyte-macrophage colony-stimulating factor or interleukin-2 with specificity for B-cell malignancies exhibit enhanced effector functions while retaining tumor targeting properties blood. 1997

Hornick, J.L., Khawli, L.A., Hu, P., Lynch, M., Anderson, P.M. and Epstein A.L.

Notes: In this study, the CytoTox® 96 Non-Radioactive Cytotoxicity Assay was used to study antibody dependent cell-mediated cytotoxicity (ADCC). ARH-77 cells, plated at 2 x 104 cells per well in a 96-well plate, were used as target cells. Antibody or fusion proteins were added to the cells at 1μg/ml and ratios of 12.5:1 to 50:1 effector:target cells were made with MNC (bone marrow mononuclear) cells or PNC (neutrophilic polymorphonuclear) leukocytes. Plates were incubated at 37°C for 4 hours, after which supernatants were analyzed for LDH release. (3014)

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Blood 89(12), 4437-4447. Chimeric CLL-1 antibody fusion proteins containing granulocyte-macrophage colony-stimulating factor or interleukin-2 with specificity for B-cell malignancies exhibit enhanced effector functions while retaining tumor targeting properties. 1997

Hornick, J.L., Khawli, L.A., Hu, P., Lynch, M., Anderson, P.M., Epstein, AL.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay activated human PBMC mediated cytotoxicity. (2866)

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