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J. Immunol. 167(11), 6286–6291. Kinetics of antigen-induced phenotypic and functional maturation of human monocyte-derived dendritic cells. 2001

Hsieh, S.M., Pan, S.C., Hung, C.C., Tsai, H.C., Chen, M.Y., Lee, C.N., Chang, SC.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess the cytolytic activity of CMV antigen-treated monocytes after incubation with CD8+ T cells at various effector to target ratios. (2891)

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J. Gen. Virol. 82(Pt 7), 1667–1675. The degree of attenuation of tick-borne encephalitis virus depends on the cumulative effects of point mutations. 2001

Gritsun, T.S., Desai, A., Gould, EA.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cytopathogenicity of tick-borne encephalitis complex virus and mutant variants. Instead of assaying the media, the researchers evaluated cell lysates. (2972)

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Blood 96(5), 1914-1920. Acute myeloblastic leukemic cells acquire cellular cytotoxicity under genotoxic stress: implication of granzyme B and perforin. 2000

Bruno, A.P., Lautier, D., d'Orgeix, A.T., Laurent, G., Quillet-Mary, A.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess effector cells (KG1a, TF1, HEL and PBL/IL-2) pretreated with cytotoxic agents then mixed with target cells at various effector-to-target ratios in triplicates. (2923)

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Mol. Biol. Cell 11(2), 453-466. Beta1 integrins regulate keratinocyte adhesion and differentiation by distinct mechanisms. 2000

Levy, L., Broad, S., Diekmann, D., Evans, R.D., Watt, FM.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used for a laminin adherence assay. (2926)

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FASEB J. 14, 1508-17. Carboxyl-terminal fragment of Alzheimer's APP destabilizes calcium homeostasis and renders neuronal cells vulnerable to excitotoxicity. 2000

Kim, H.S., Park, C.H., Cha, S.H., Lee, J.H., Lee, S., Kim, Y., Rah, J.C., Jeong, S.J. and Suh, Y.H.

Notes: The CytoTox® 96 Non-Radioactive Cytotoxicity Assay was used to determine the toxicity of two peptides, a C-terminal fragment of APP (CT 105), and Amyloid β protein (Aβ 1-42). Cells were treated with the peptides in the presence or absence of neuroprotective agents cholesterol, MK801, nifedipine, or verapamil. Studies were done in PC12 cells and in SK-N-SH human neuroblastoma cells. (2134)

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FASEB J. 14(3), 555-564. Endothelial preconditioning by transient oxidative stress reduces inflammatory responses of cultured endothelial cells to TNF-alpha. 2000

Zahler, S., Kupatt, C., Becker, BF.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay IL -6 and IL-8 mediated cytotoxicity. (2916)

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J. Virol. 74(13), 5997-6005. Enhancement of primary and secondary cellular immune responses against human immunodeficiency virus type 1 gag by using DNA expression vectors that target Gag antigen to the secretory pathway. 2000

Qiu, J.T., Liu, B., Tian, C., Pavlakis, G.N., Yu, XF.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay activated mouse splenocyte mediated cytotoxicity. (2957)

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J. Cell Biol. 150, 165-175. Glutamate slows axonal transport of neurofilaments in transfected neurons. 2000

Ackerley, S., Grierson, A.J., Brownlees, J., Thornhill, P., Anderton, B.H., Leigh, P.N., Shaw, C.E., and Miller C.C.

Notes: The authors seek to determine the role of glutamate in excitotoxicity and neurofilament accumulation seen in some neurodegenerative diseases. Neurofilament light, middle, and heavy chains were expressed from rat cDNAs cloned into the pCI-neo Mammalian Expression vector in SW-13 cells. Primary rat cortical neurons were transfected with a neurofilament middle chain and green fluorescent fusion protein. SW13 cells and primary rat cortical neurons were transfected with the ProFection® Mammalian Transfection System–Calcium Phosphate. The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to monitor glutamate toxicity in these cell. To determine the role of the MAPK and JNK signaling pathways,  SW13- cells and primary neuronal cells were immunostained for dually phosphorylated MAPK and JNK using Promega's Anti-ACTIVE® MAPK pAb and Anti-ACTIVE® JNK pAb, respectively. Cells were fixed in 4% paraformaldehyde, permeabilized in 0.1% Triton® X-100 in PBS, blocked with  0.2% Tween® 20 in TBS, and incubated with primary antibodies diluted in blocking solution. Western blot analyses were performed on the primary cortical neurons to quantitate the level of dually phosphorylated MAPK protein  The blots were also probed with a pan MAPK antibody that detects total (active and inactive) MAPK. (2382)

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J. Immunol. 165(7), 4120 – 4126. Impairment of STAT activation by IL-12 in a patient with atypical mycobacterial and staphylococcal infections. 2000

Gollob, J.A., Veenstra, K.G., Jyonouchi, H., Kelly, A.M., Ferrieri, P., Panka, D.J., Altare, F., Fieschi, C., Casanova, J.L., Frank, D.A., Mier, JW.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cytotoxicity using human PBMC targeting K562 cells. Whole PBMC first incubated overnight in U-bottom 96-well plates with medium alone, 1 nM IL-12, 100 U/ml IL-2, or IL-12 plus IL-2. Then K562 cells were then added to the PBMC at a 10:1 E:T cell ratio and incubated for 4 hours. (2924)

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J. Nutr. 130(1), 111–115. Implanted tumor growth is suppressed and survival is prolonged in sixty percent of food-restricted mice. 2000

Matsuzaki, J., Yamaji, R., Kiyomiya, K., Kurebe, M., Inui, H., Nakano, Y.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cell-mediated cytotoxicity of natural killer cells isolated from mice that were food restricted after injection with tumor-inducing cells. The NK cells were coincubated with splenocytes at 100:1 effector to target ratio. (2988)

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J. Neurochem. 74(5), 2079-2086. In vitro antioxidant neuroprotective activity of BN 80933, a dual inhibitor of neuronal nitric oxide synthase and lipid peroxidation. 2000

Demerle-Pallardy, C., Gillard-Roubert, V., Marin, J.G., Auguet, M., Chabrier, PE.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay the affect of various compounds on hypoxia-induced cytotoxicity. (2887)

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J. Lipid Res. 41(12), 1969-1979. Modulation of hepatic lipoprotein synthesis and secretion by taxifolin, a plant flavonoid. 2000

Theriault, A., Wang, Q., Van, Iderstine, S.C., Chen, B., Franke, A.A., Adeli, K.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess the cytotoxic effect of taxifolin on cells without compromising viability. (2908)

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J. Biol. Chem. 275(8), 5535-5544. Phosphorylation of microtubule-associated protein tau is regulated by protein phosphatase 2A in mammalian brain. Implications for neurofibrillary degeneration in Alzheimer's disease. 2000

Gong, C.X., Lidsky, T., Wegiel, J., Zuck, L., Grundke-Iqbal, I., Iqbal, K.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay response of rat brain slices to protein phosphatase inhibitors. (2877)

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J. Leukoc. Biol. 68(1), 131-136. Proteasome-mediated regulation of interleukin-1beta turnover and export in human monocytes. 2000

Moors, M.A., Mizel, S.B.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay if proteosome inhibitors were cytotoxic to cells. (2993)

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Antimicrob. Agents Chemother. 44(6), 1588-1597. Recombinant green fluorescent protein-expressing human cytomegalovirus as a tool for screening antiviral agents. 2000

Marschall, M., Freitag, M., Weiler, S., Sorg, G., Stamminger, T.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay Zn2+ mediated cytotoxicity. (2900)

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Brain Res. 853(2), 299-309. Regulation of phosphorylation of neuronal microtubule-associated proteins MAP1b and MAP2 by protein phosphatase-2A and -2B in rat brain. 2000

Gong, C.X., Wegiel, J., Lidsky, T., Zuck, L., Avila, J., Wisniewski, H.M., Grundke-Iqbal, I., Iqbal, K.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay response of rat brain slices to protein phosphatase inhibitors. (2876)

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Infect. Immun. 68(10), 5702–5709. Salmonella pathogenicity island 1-independent induction of apoptosis in infected macrophages by Salmonella enterica serotype typhimurium. 2000

van, der, Velden, A.W., Lindgren, S.W., Worley, M.J., Heffron, F.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cell cytotoxicity after infection with late-log or stationary phase Salmonella typhimurium and various strains. (2920)

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Br. J. Pharmacol. 131(7), 1429 - 1437. Selective mGluR5 antagonists MPEP and SIB-1893 decrease NMDA or glutamate-mediated neuronal toxicity through actions that reflect NMDA receptor antagonism. 2000

O'Leary, D.M., Movsesyan, V., Vicini, S., Faden, AI.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess the cellular cytotoxicity when treated with either lovastatin or cerivastatin. (2903)

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J. Neurochem. 74(1), 60-69. Spermine-induced toxicity in cerebellar granule neurons is independent of its actions at NMDA receptors. 2000

Segal, J.A., Skolnick, P.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay spermine and Glutamate mediated cytotoxicity. (2952)

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EMBO J. 19, 3325-3336. The apoptotic signaling pathway activated by Toll-like receptor-2 2000

Aliprantis, A.O., Yang, R.-B., Weiss, D.S., Godowski, P. and Zychlinsky, A.

Notes: Apoptosis was studied by TUNEL assay using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL System) and PI staining. Amount of apoptosis was quantitated by flow cytometry. Test compounds that activate the TLR2 pathway were tested for cytotoxicity using the CytoTox 96® Non-Radioactive Cytotoxicity Assay. The cells were at 2.4x104 cells/well. (2128)

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Neuroscience 91(3), 1171-1181. A study of the mechanism of the release of ATP from rat cortical astroglial cells evoked by activation of glutamate receptors. 1999

Queiroz, G., Meyer, D.K., Meyer, A., Starke, K., von, Kugelgen, I.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay glutamate receptor agonist mediated cytotoxicity. (2868)

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Science 285, 736-739. Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2. 1999

Aliprantis, A.O., Yang, R.-B., Mark, M.R., Suggett, S., Devaux, B., Radolf, J.D., Klimpel, G.R., Godowski, P. and Zychlinsky, A.

Notes: 293 cells stably expressing the human toll-like receptor-2 were challenged with various agents and analyzed for apoptosis. One test was a visual inspection for blebbing and the other was quantitation by TUNEL staining, using the Apoptosis Detection System, Fluorescein (DeadEnd™ Fluorometric TUNEL Assay), followed by FACS analysis. In another assay for THP-1 cell death, the CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to monitor LDH release in response to bacterial lipoproteins. (1508)

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Proc. Natl. Acad. Sci. USA 96(20), 11358-11363. Cholesterol efflux to apolipoprotein AI involves endocytosis and resecretion in a calcium-dependent pathway. 1999

Takahashi, Y., Smith, JD.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay 8-bromo-cAMP mediated cytotoxicity. (2975)

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J. Biol. Chem. 274(30), 20909-20915. Cytotoxicity and site-specific DNA damage induced by nitroxyl anion (NO(-)) in the presence of hydrogen peroxide. Implications for various pathophysiological conditions. 1999

Chazotte-Aubert, L., Oikawa, S., Gilibert, I., Bianchini, F., Kawanishi, S., Ohshima, H.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assay hydrogen Peroxide; Angeli's salt, and Diethylamine-NONOate mediated cytotoxicity. (2943)

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Eur. J. Biochem. 264(2), 569-576. Determinants in the N-terminal domains of galectin-3 for secretion by a novel pathway circumventing the endoplasmic reticulum-Golgi complex. 1999

Menon, R.P., Hughes, RC.

Notes: The CytoTox 96® Non-Radioactive Cytotoxicity Assay was used to assess cell lysis after pulse chase experiments were used to assay secretion of chimeric proteins. (2888)

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