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Promega Corporation

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Assay Drug Dev. Technol. 2, 51-62. Use of multiple assay endpoints to investigate the effects of incubation time, dose of toxin, and plating density in cell-based cytotoxicity assays. 2004

Riss, T.L. and Moravec, R.A.

Notes: The effects of tamoxifen and vinblastine were assayed on HepG2 and HL-60 cells using a variety of Promega’s cell-based assays. The CellTiter-Glo® Luminescent Cell Viability Assay, CellTiter 96® AQueous One Solution Cell Proliferation Assay, and CellTiter-Blue® Cell Viability Assay were used to test the effects of 0-100µM tamoxifen on HepG2 cell viability after an incubation period ranging from 0-24 hours. Cell membrane integrity was assessed with the CytoTox-ONE™ Homogeneous Membrane Integrity Assay. The Caspase-Glo® 3/7 Assay was also used to measure Caspase 3/7 activity after 0-100µM tamoxifen treatment. (3189)

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Clin. Can. Res. 9, 2933-2939. Expression of constitutively active Akt-3 in MCF-7 breast cancer cells reverses the estrogen and tamoxifen responsivity of these cells in vivo 2003

Faridi, J., Wang, L., Endermann, G., Roth, R.A.

Notes: MCF-7 cells were transfected with a plasmid encoding constitutively active Akt-3. To assess estrogen-related transcriptional activity, the cells were transfected with an ERE-luciferase (firefly) reporter and a Renilla luciferase control plasmid. Dual luciferase assays were performed to determine the effect of estrogen on transcription in these Akt-3 expressing cells. Additionally, proliferation assays were performed using the CellTiter® AQueous One Solution Cell Proliferation Assay and showed that the Akt-3 expression confers serum-independent growth on the cells. (2709)

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Assay Drug Dev. Technol. 3, 469-477. HTS Compatible Assay for Antioxidative Agents Using Primary Cultured Hepatocytes 2003

Gaunitz, F. and Heise, K.

Notes: Primary hepatocytes from Sprauge-Dawley rats were incubated with various concentrations of tert-butylhydroperoxide to induce oxidative stress and then cell viability, cytotoxicity and apoptosis were assayed with Promega's CellTiter 96 AQueous One Solution Cell Proliferation Assay, CellTiter-Blue Cell Viability Assay, CellTiter-Glo Luminescent Cell Viability Assay, CytoTox-ONE Homogeneous Membrane Integrity Assay, Apo-ONE Homogeneous Caspase-3/7 Assay and assay systems. (2969)

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Clin. Can. Res. 9, 1528-1534. Possible role of placental leucine aminopeptidase in the antiproliferative effect of oxytocin in human endometrial adenocarinoma 2003

Suzuki, Y., Shibata, K., Kikkawa, F., Kajiyama, H., Kazuhiko, I., Nomura, S., Tsujumoto, M., Mizutani, S.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay  was used to evaluate the effect of oxytocin on proliferation of human endometrial  endometriod adenocarcinoma cell lines. (2654)

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J. Biol. Chem. 278 (52), 52307-52314. Silencing of RNA helicase II/Gu inhibits mammalian ribosomal RNA production. 2003

Henning, D., So, R.B., Jin, R., Lau, L.F. and Valdez, B.C.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to analyze the effect of siRNA transfection on HeLa cell proliferation. HeLa cells were transfected twice with 10nM of a siRNA for RNA helicase II/Guα, a scrambled sequence siRNA, or mock transfected with the transfection reagent. Transfected cells (50,000-100,000) were seeded in 6-well culture plates for 72 hours. After this incubation, cells were plated at 625-20,000 cells per well in 96-well culture plates, and were then assayed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay.  (3036)

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Clin. Can. Res. 8, 1234-1240. Induction of apoptosis by bexarotene in cutaneous T-Cell lymphoma cells: Relevance to mechanism of therapeutic action 2002

Zhang, C., Hazarika, P., Ni, X., Weidner, D., Duvic, M.

Notes: The authors investigated the mechanism of action of the synthetic rexinoid, bexarotene, in the treatment of cutaneous T-Cell Lymphoma (CTCL). They used the CellTiter 96® AQueous One Solution Cell Proliferation Assay to assess the effect of bexarotene on three human CTCL cell lines (MJ, Hut78 and HH). Cells were treated with or without 0.1, 1, or 10μM bexarotene for varying time periods. Results indicated significant inhibition of cell growth as dosage increased. (2448)

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Br. J. Cancer 87, 98-105. Novel substituted methylenedioxy lignan suppresses proliferation of cancer cells by inhibiting telomerase and activation of c-myc and caspases leading to apoptosis. 2002

Giridharan, P., Somasundaram, S.T., Perumal, K., Vishwakarma, R.A., Karthikeyan, N.P., Velmurugan, R., and Balakrishnan, A.

Notes: In this paper, the CellTiter 96® Aqueous Assay was used to assess cell proliferation. (2525)

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Nature 418, 386. Screening inhibitors of anthrax lethal factor. 2002

Tonello, F., Seweso, M., Marin, O., Mock, M., and Montecucco, C.

Notes: This brief communication discusses substrates of anthrax lethal factor that can be used for high-throughput screening of potential inhibitors. The CellTiter® Aqueous Cell Proliferation Assay was used to assess the effect of selected inhibitors on cytotoxicity of lethal factor in RAW264.7 cells. (2555)

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Clin. Can. Res. 8, 2448-2454. Selective inhibition of epidermal growth factor receptor by ZD1839 decreases the growth and invasion of ovarian clear cell adenocarcinoma cells 2002

Fujimura, M. Hidaka, T. and Shigeru, S.

Notes: The authors investigated the effect of several growth factors that bind tyrosine kinase receptors on the growth and invasion of three ovarian clear cell carcinoma cell lines. They used the CellTiter® AQueous One Solution Cell Proliferation Assay to measure cell proliferation after treatments. The epidermal growth factor ZD1839 showed inhibitory effects. (2496)

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Mol. Cell. Biol. 2001, 8385-8397. Neuropeptide-induced androgen independence in prostate cancer cells: Roles of noreceptor tyrosine kinases Etk/Bmx, Src, and Focal Adhesion Kinase 2001

Lee, L-F., Guan, J., Qiu, Y., Kung, H-J.

Notes: The authors investigated the inhibitory effect of flutamide on bombesin-induced cell proliferation in LNCaP prostate epithelial cells. Cells were preincubated in the presence or absence of flutamide and then treated with either R1881 (control) or bombesin for 72 hours under charcoal-stripped serum conditions. Cell proliferation was measured using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2527)

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J. Neurochem. 74, 2278-2287. 4-Hydroxynonenal induces oxidative stress and death of cultured spinal cord neurons 2000

Malecki, A., Garrido, R., Mattson, M.P., Hennig, B., Toborek, M.

Notes: The authors treated mouse spinal cord neurons with different doses of 4-hydroxynonenal (HNE) and assessed cytotoxicity using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (2533)

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J. Biol. Chem. 275, 9095-9098. Activated protein C directly activates human endothelial gelatinase A. 2000

Nguyen, M. , Arkell, J. , Jackson, C.J.

Notes: In this paper, the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to normalize data to the number of cells present in each well. Equal numbers of cells were grown in each well and the conditioned media removed for enzymatic assays. The relative number of human umbilical vein endothelial cells remaining were measured with the assay. (0608)

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J. Immunol. 165(5), 2500-2510. Apoptosis induced by TGF-beta 1 in Burkitt's lymphoma cells is caspase 8 dependent but is death receptor independent. 2000

Inman, G. J. , and Allday, M. J.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the ability of different soluble receptors to block apoptotic agents. A soluble DR5/Fc fusion blocked TRAIL-mediated apoptosis but not TGFbeta1-mediated apoptosis. (0011)

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J. Biol. Chem. 275, 8772-8778. Chaperones Hsp70 and Hsp40 suppress aggregate formation and apoptosis in cultured neuronal cells expressing truncated androgen receptor protein with expanded polyglutamine tract. 2000

Kobayashi, Y., Kume, A., Li, M., Doyu, M., Hata, M., Ohtsuka, K., Sobue, G.

Notes: The DeadEnd™ Colorimetric Apoptosis Detection System was used to detect apoptosis in transfected Neuro 2a cells. The cells were transfected with truncated androgen receptors that aggregate in either the nuclei or the cytoplasm. The cells with nuclear aggregates were apoptotic and those with cytoplasmic aggregates were mostly nonapoptotic. The location of the aggregates was determined with GFP fusions. The basic protocol of the DeadEnd™ Colorimetric Apoptosis Detection System was modified to use streptavidin-Texas Red® for fluorometric detection rather than colorimetric detection. Good detail is provided for the modifications. Neuro 2a cells expressing the aggregating AR mutations as well as various chaperonins were analyzed for cell viability at 24, 48 and 72 hours with the CellTiter 96® AQueous One Solution (MTS/PES). The name of the DeadEnd™ Colorimetric Apoptosis Detection System has been changed to DeadEnd™ Colorimetric TUNEL System. (0913)

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FASEB J. 14, 1801-1813. Definition of endotoxin binding sites in horseshoe crab Factor C recombinant sushi proteins and neutralization of endotoxin by sushi peptides. 2000

Tan, N.S., Ng, M.L., Yau, Y.H., Chong, P.K., Ho, B. and Ding, J.L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to assess toxicity of peptides in cell culture. The cells were THP-1 monocytes, and the peptide concentrations used were 1.25 to 320µM. (2127)

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Brain Res. 857, 265-274. Glial growth factor 2 induces proliferation and structural changes in ensheathing cells. 2000

Chuah, M. I. , Cossins, J. , Woodhall, E. , Tennent, R. , Nash, G. , and West, A. K.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to examine the proliferation of rat olfactory bulb ensheathing cells to glial growth factor 2. (0009)

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FASEB J. 14, 859-870. High-affinity LPS binding domain(s) in recombinant factor C of a horseshoe crab neutralizes LPS-induced lethality 2000

Tan, N.S., Ho, B., Ding, J.L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to measure the cytotoxic effect of a biologically active, recombinant fragment of factor C. Factor C is an endotoxin-sensitive serine protease. Various concentrations of the factor C fragment were added to THP-1 cells and allowed to react for 60 minutes prior to the assay. (2545)

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Neurosci. Lett. 288(1), 37-40. Induction of rat L-phosphoserine phosphatase by amyloid-beta (1-42) is inhibited by interleukin-11. 2000

Heese, K. , Nagai, Y. , and Sawada, T.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to judge the percent survival of B104 rat neuroblastoma cells in response to beta-amyloid peptides as well as glutamate and serine combinations. (0015)

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Brain Res. Dev. Brain Res. 122, 97-109. Inhibition of mitogen-activated protein kinase kinase blocks proliferation of neural progenitor cells. 2000

Learish, R.D., Bruss, M.D., and Haak-Frendscho, M.

Notes: A primary cell line from rat subventricular zone which remains undifferentiated over time was developed as a model for MAPK activation. Cell were stimulated with 20 ng/ml bFGF and 20 ng/ml EGF (Promega) to activate MAPK. The MAPK pathway was inhibited with either U0126 (Promega) or  PD98059. Immunocytochemistry was performed with the Anti-ERK 1/2 pAb (1:100 dilution) to detect both active and inactive forms of MAPK proteins and with the Anti-ACTIVE™ MAPK pAb (1:100 dilution) to specifically detect the dually phosphorylated, active forms of MAPK. Cells were also immunostained with the neuron specific marker Anti-III-tubulin mAb (0.5 µg/ml). Cell proliferation was monitored with the CellTiter 96® AQueous One Solution Cell Proliferation Assay System. Apoptosis within the cell population was monitored using the DeadEnd™ Fluorometric TUNEL System. (2391)

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J. Clin. Invest. 106(4), 501-509. Leptin enhances wound re-epithelialization and constitutes a direct function of leptin in skin repair. 2000

Frank, S. , Stallmeyer, B. , Kampfer, H. , Kolb, N. , and Pfeilschifter, J.

Notes: Proliferation of HaCat and primary human keratinocytes to EGF, KGF and leptin was examined with the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES). (0012)

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Proc. Natl. Acad. Sci. USA 97(10), 5416-5421. Myogenic stem cell function is impaired in mice lacking the forkhead/winged helix protein MNF. 2000

Garry, D. J. , Meeson, A. , Elterman, J. , Zhao, Y. , Yang, P. , Bassel Duby, R. , and Williams, R. S.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the proliferation of primary myocytes derived from normal and knockout mice to serum. (0017)

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Neurosci. Lett. 282(3), 153-156. P2 Purinoceptor expression and functional changes of hypoxia-activated cultured rat retinal microglia. 2000

Morigiwa, K. , Quan, M. , Murakami, M. , Yamashita, M. , and Fukuda, Y.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the survival of the rat retinal microglia to P2 receptor agonists and antagonists. (0010)

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Brain Res. 868(2), 230-240. Parathyroid hormone-related protein is expressed by transformed and fetal human astrocytes and inhibits cell proliferation. 2000

Shankar, P. P. , Wei, H. , Davee, S. M. , and Funk, J. L.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to show that TNFalpha nor EGF were toxic to cultured primary human astrocytes or U-373 MG transformed human astrocytoma cells. (0016)

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Mol. Cell 6(1), 99-108. Site-specific serine phosphorylation of the IL-3 receptor is required for hemopoietic cell survival. 2000

Guthridge, M. A. , Stomski, F. C. , Barry, E. F. , Winnall, W. , Woodcock, J. M. , McClure, B. J. , Dottore, M. , Berndt, M. C. , and Lopez, A. F.

Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS/PES) was used to assess the response of transfected CTL-EN cells (a IL-2 dependent subline of CTLL-2 cells) to IL-3. The cells were transfected with either wildtype or mutant IL-3 receptor subunits. (0014)

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J. Biol. Chem. 275, 17605-17610. Targeted inactivation of the isoprenylcysteine carboxyl methyltransferase gene causes mislocalizatioin of K-Ras in mammalian cells 2000

Bergo, M.O., Leung, G.K., Ambroziak, P. Otto, J.C., Casey, P.J., and Young, S.G.

Notes: The CellTiter 96® AQueous One Solution Assay was used to look at the differential proliferation of normal and knockout isoprenylcysteine carboxyl methyltransferase cells. The normal cells proliferated at a greater rate. (2485)

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