Henning, D., So, R.B., Jin, R., Lau, L.F. and Valdez, B.C.
Notes: The CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to analyze the effect of siRNA transfection on HeLa cell proliferation. HeLa cells were transfected twice with 10nM of a siRNA for RNA helicase II/Guα, a scrambled sequence siRNA, or mock transfected with the transfection reagent. Transfected cells (50,000-100,000) were seeded in 6-well culture plates for 72 hours. After this incubation, cells were plated at 625-20,000 cells per well in 96-well culture plates, and were then assayed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay. (3036)