Johnston, S.D., Yu, X.M. and Mertz, J.E.
Notes: Labeled TBP, Rb, E1a, Sp1, TFIIB, TEF-1,SV40 large T antigen (Tag) and BMV were synthesized with [35S]methionine in the TNT® Coupled Reticulocyte Lysate System. Proteins were assayed for binding to GST-Tag fusion protein. Tag interacts with TBP, TFIIB, Sp1, TEF-1 and the 140kDa subunit of RNA polymerase. Because binding involves the same region of the protein, it probably does not transactivate via concurrent interactions with multiple proteins. (1798)