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Biochemistry 35, 11612-11621. Nuclear multicatalytic proteinase subunit RRC3 is important for growth regulation in hepatocytes. 1996

Benedict, C.M. and Clawson, G.A.

Notes: RRC3 constructs with an HA epitope ligated to the C-terminal end were in vitro transcribed and translated using the TNT® T7 Coupled Reticulocyte Lysate System. The proteins were immunoprecipitated with mouse anti-HA. Stability of the different RRC3 proteins was examined by mixing transcription/translation reactions with cytosol. Proteins from all constructs were equally stable with little degradation. (1759)

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J. Biol. Chem. 271, 17167-17173. Peroxisome proliferator-activated receptor alpha inhibits hepatic S14 gene transcription. 1996

Ren, B., Thelen, A. and Jump, D.B.

Notes: Mouse peroxisome proliferator-activated receptor alpha (PPARalpha) and retinoid X receptor (RXRalpha) were synthesized in vitro with the TNT® Coupled Reticulocyte Lysate System. Gel shifts were performed using [32P]labeled acyl-CoA oxidase oligo. PPARalpha and RXRalpha bind the AOX-PPRE as a heterodimer, but neither binds the AOX-PPRE alone. PPARalpha inhibits TRbeta/RXRalpha heterodimer formation on a DR+4 T3 response element. (1815)

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Hum. Mutat. 8, 236-246. PKU mutation (D143G) associated with an apparent high residual enzyme activity: expression of a kinetic variant form of phenylalanine hydroxylase in three different systems. 1996

Knappskog, P.M., Eiken, H.G. , Martinez, A., Bruland, O., Apold, J., Flatmark, T.

Notes: A point mutation in the tyrosine hydroxylase (hTH) enzyme is responsible for a form of dystonia. Mutant and wildtype hTH were expressed by the TNT® Coupled Reticulocyte Lysate System. Characterization of the mutant enzyme reveals a reduction in activity to approximately 15% of wildtype. (0910)

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Nucl. Acids Res. 24, 3607-3613. Protein-protein interaction between the transcriptional repressor E4BP4 and the TBP-binding protein Dr1. 1996

Cowell, I. and Hurst, H.

Notes: Topoisomerase I, Dr1 (a TBP repressor) and various E4BP4 deletion mutant proteins were generated in the TNT® Coupled Reticulocyte Lysate System using promoters SP6, T3 or T7. The proteins were [35S]methionine-labeled and used in GST pull down assays. E4BP4 mutants that are unable to repress transcription are deficient in Dr1 binding. (1791)

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Hum. Mol. Genet. 5, 1023-1028. Recessively inherited L-DOPA-responsive parkinsonism in infancy caused by a point mutation (L205P) in the tyrosine hydroxylase gene. 1996

Ludecke, B., Knappskog, P.M., Clayton, P.T., Surtees, R.A., Clelland, J.D., Heales, S.J., Brand, M.P., Bartholome, K., Flatmark, T.

Notes: A point mutation in the tyrosine hydroxylase (hTH) enzyme is responsible for a form of dystonia. Mutant and wild type hTH were expressed by the TNT® Coupled Reticulocyte Lysate System. Characterization of the mutant enzyme reveals a reduction in activity to approximately 15% of wildtype. (0750)

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Cell 87, 437-446. Regions in beta-chemokine receptors CCR5 and CCR2b that determine HIV-1 cofactor specificity. 1996

Rucker, J., Samson, M., Doranz, B.J., Libert, F., Berson, J.F., Yi, Y., Smyth, R. J., Collman, R.G., Broder, C.C., Vassart, G., Doms, R.W., Parmentier, M.

Notes: The Luciferase T7 Control DNA from the TNT® Coupled Reticulocyte Lysate System was used to look at cofactors to CD4 necessary for entry of HIV-1. HeLa cells were infected with recombinant vaccinia virus vectors expressing the protein of interest and the T7 RNA Polymerase. Quail QT6 cells were transfected with plasmids encoding CD4, the desired cofactor, and the Luciferase T7 Control DNA. Fusion of the two cells was then quantitated by luciferase activity. (0462)

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Nucl. Acids Res. 24, 2176-2182. Regulation of in vitro gene expression using antisense oligonucleotides or antisense expression plasmids transfected using starburst PAMAM dendrimers. 1996

Kukowska Latallo, J.F., Johnson, J., Tomalia, D.A., Baker, J.R., Jr.

Notes: [35S]methionine-labeled RalGDS (guanine nucleotide dissociation stimulator) was transcribed and translated in the TNT® T7 Coupled Reticulocyte Lysate System. The lysate was incubated with GTPase samples and used in immunoprecipitation assays with anti-RalGDS antisera. GST-R-ras, GST-H-ras and GST-Rap (Ras-like GTPases) bind with full-length RalGDS in a GTP-dependent manner. (0860)

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Am. J. Hum. Genet. 59, 818-824. RNA-based mutation screening in hereditary nonpolyposis colorectal cancer. 1996

Kohonen-Corish, M., Ross, V.L., Doe, W.F., Kool, D.A., Edkins, E., Faragher, I., Wijnen, J., Khan, P.M., Macrae, F. and St. John, D.J.

Notes: This group evaluated RNA-based screening methods for detection of mutations in hMLH1 and hMLH2. Their results suggest that PTT is useful for preliminary screening tests to localize a mutation, which then can be confirmed by sequencing. The PTT was performed using T7 polymerase and the TNT® T7 Coupled Reticulocyte Lysate System. (0875)

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Oncogene 13, 1353-1357. Screening for mutations in exon 11 of the BRCA1 gene in 70 Italian breast and ovarian cancer patients by protein truncation test. 1996

De Benedetti, V. M. , Radice, P. , Mondini, P. , Spatti, G. , Conti, A. , Illeni, M. T. , Caligo, M. A. , Cipollini, G. , Bevilaqua, G. , Pilotti, S. , Pierotti, M. A.

Notes: The protein truncation tese (PTT) was used to analyze BRCA1 exon 11, which includes ~61% of the coding region of the gene. The observed frequencies of mutations in the BRCA1 gene were not significantly different from expected, based upon phenotype and family history. PTT was performed using the TNT® T7 Coupled Reticulocyte Lysate System. Each mutation was confirmed by an independent PCR amplification and PTT analysis. (1258)

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EMBO J. 11, 2760-2770. Selective interaction of JNK protein kinase isoforms with transcription factors. 1996

Gupta, S., Barrett, T., Whitmarsh, A.J., Cavanagh, J., Sluss, H.K., Derijard, B. and Davis, R.J.

Notes: [35S]labeled JNK2alpha2 protein was transcribed and translated in vitro using the TNT® T7 Coupled Reticulocyte Lysate System for use in binding assays with GST-c-Jun, GST-JunB and GST-JunD. The relative binding of JNK2alpha2 to c-Jun was highest, followed by JNK2alpha2 binding to GST-JunB. The binding of JNK2alpha2 to GST-JunD was extremely weak. The results indicate that c-Jun is an excellent substrate for and binds to JNK. Although Jun-B binds JNK, it is not a substrate for JNK. (1794)

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J. Med. Genet. 33, 935-939. Splicing mutations in DMD/BMD detected by RT-PCR/PTT: detection of a 19AA insertion in the cysteine rich domain of dystrophin compatible with BMD. 1996

Roest, P.A., Bout, M., van der Tuijn, A.C , Ginjaar, I.B., Bakker, E., Hogervorst, F. B., van Ommen, G.J., den Dunnen, J.T.

Notes: RT-PCR and the Protein Truncation Test (PTT) were used to identify DMD/BMD-associated point mutations that were not detected by DNA-based methods. The RNA method is more sensitive because many of the primers used for methods such as multiplex PCR do not permit the detection of mutations affecting splice sites. The RNA method used in this study screened the total coding region. PTT was performed with the TNT® Coupled Reticulocyte Lysate System. (0491)

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J. Biol. Chem. 271, 10405-10412. Suppression of the human erythropoietin gene expression by the TR2 orphan receptor, a member of the steroid receptor superfamily. 1996

Lee, H.J., Young, W.J., Shih, C.Y. and Chang, C.

Notes: The TR2 orphan receptor was produced in vitro using the TNT® Coupled Reticulocyte Lysate System. The protein was analyzed by SDS-PAGE and EMSAs. The binding affinity of TR2 for the +55 region of SV40 was then calculated using the ratio between specific DNA-protein binding and free DNA probe. (1841)

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J. Virol. 70, 3706-3715. Synthesis and assembly of retrovirus Gag precursors into immature capsids in vitro. 1996

Sakalian, M., Parker, S.D., Weldon, R.A. Jr, Hunter, E.

Notes: Gag polyproteins were synthesized using the TNT® Coupled Reticulocyte Lysate System in the presence of [35S]methionine. Sedimentation into sucrose gradients and electron microscopy of the lysates were performed to determine if capsid assembly could occur in vitro. The assembly of capsids in this in vitro system does occur and is sensitive to the same mutations that block assembly in vivo. (0430)

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Cell 84, 587-597. The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers. 1996

Ohta, M. , Inoue, H. , Cotticelli, M. G. , Kastury, K. , Baffa, R. , Palazzo, J. , Siprashvili, Z. , Mori, M. , McCue, P. , Druck, T. , et al.

Notes: These researchers cloned the FHIT gene by PCR and expressed it in the TNT® Coupled Reticulocyte Lysate System. Synthesized proteins were analyzed using SDS-PAGE and autoradiography. (0596)

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EMBO J. 15, 4613-4628. The fission yeast Cdc1 protein, a homologue of the small subunit of DNA polymerase delta, binds to Pol3 and Cdc27. 1996

MacNeill, S.A., Moreno, S., Reynolds, N., Nurse, P. and Fantes, P.A.

Notes: HA and Myc epitope tags were introduced at the C-terminus of Cdc1 and Cdc27 by oligo-directed mutagenesis. Cdc1tag and Cdc27tag were cloned into the pGEM®-4Z Vector and expressed in the TNT® T7 Coupled Reticulocyte Lysate System ± [35S]methionine. In GST coimmunoprecipitation assays, Cdc1 interacts with GST-Pol3, and the GST-Pol3 binds preferentially to a truncated Cdc1 translation product. To confirm an interaction observed between Cdc1 and Cdc27, the TNT®-expressed proteins were mixed together and co-immunoprecipitated using the anti-Cdc27 antibodies Using EGS (ethylene glycol-bis-succinimidylsuccinate) labeled, in vitro translated Cdc1 and Cdc27 were cross-linked. (1771)

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J. Biol. Chem. 271, 14800-14806. The major astrocytic phosphoprotein PEA-15 is encoded by two mRNAs conserved on their full length in mouse and human. 1996

Estelles, A. , Yokoyama, M. , Nothias, F. , Vincent, J. D. , Glowinski, J. , Vernier, P. , Chneiweiss, H.

Notes: PEA-15 cDNAs were cloned from a mouse astrocytic library, and the clones were linearized and expressed using T3 or T7 Polymerase in the TNT® Reticulocyte Lysate System either in the presence of [35S]methionine or Transcend™ tRNA. The resulting proteins were analyzed by SDS-PAGE and Western blotting, respectively. A unique 15kDa protein that was recognized by an antibody to PEA-15 was produced in the TNT® System. (1178)

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J. Virol. 70, 1191-1202. The major transcriptional transactivation domain of Simian virus 40 large T antigen associates nonconcurrently with multiple components of the transcriptional preinitiation complex. 1996

Johnston, S.D., Yu, X.M. and Mertz, J.E.

Notes: Labeled TBP, Rb, E1a, Sp1, TFIIB, TEF-1,SV40 large T antigen (Tag) and BMV were synthesized with [35S]methionine in the TNT® Coupled Reticulocyte Lysate System. Proteins were assayed for binding to GST-Tag fusion protein. Tag interacts with TBP, TFIIB, Sp1, TEF-1 and the 140kDa subunit of RNA polymerase. Because binding involves the same region of the protein, it probably does not transactivate via concurrent interactions with multiple proteins. (1798)

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J. Biol. Chem. 271, 7654-7658. The nuclear location signal of lymphoid enhancer factor-1 is recognized by two differentially expressed Srp1-nuclear localization sequence receptor proteins. 1996

Prieve, M.G., Guttridge, K.L., Munguia, J.E. and Waterman, M.L.

Notes: [35S]hLEF-1 (human lymphoid enhancer factor-1) protein fragments were generated in the TNT® Coupled Reticulocyte Lysate System. These proteins were incubated with GST-pendulin and GST-mSrp1 bound to glutathione Sepharose beads. Mouse pendulin and mSrp1 bind to the B box in the HMG DNA binding domain of hLEF-1. (1811)

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J. Biol. Chem. 271, 1935-1940. The sequence Glu1811–Lys1818 of human blood coagulation factor VIII comprises a binding site for activated factor IX. 1996

Lenting, P.J., van de Loo, J.W., Donath, M.J., van Mourik, J.A. and Mertens, K.

Notes: In this paper, transcription/translation was performed using the TNT® Coupled Reticulocyte Lysate System in the presence of [35S]methionine. Factor VIII fragments were incubated with mAbs and subjected to electrophoresis on 12% denaturing gels. These fragments helped to localize the epitope of the CLB-CagA antibody. (1766)

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Virology 221, 44-53. The transactivation and DNA binding domains of the BPV-1 E2 protein have different roles in cooperative origin binding with the e1 protein. 1996

Winokur, P. and McBride, A.

Notes: [35S]labeled E1 and E2 were expressed in the TNT® Coupled Reticulocyte Lysate System. The proteins were analyzed by SDS-PAGE and then used in DNA-protein immunoprecipitation assays. The E1 and E2 proteins were also tested for interaction in DNA binding assays. The E1 and E2 proteins cooperatively bind the origin, and this event requires E2 binding sites. (1835)

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Biochem. J. 315, 901-908. Tissue specific and androgen-regulated expression of human prostate-specific transglutaminase. 1996

Dubbink, H.J. , Verkaik, N.S. , Faber, P.W. , Trapman, J., Schroder, F.H., Romijn, J.C.

Notes: Transcription/translation of circular template was performed using the TNT® T7 Coupled Reticulocyte Lysate System. The results helped to characterize the human prostate-restricted transglutaminase. (1201)

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Hum. Genet. 98, 328-332. Transcript analysis of CFTR frameshift mutations in lymphocytes using the reverse transcription-polymerase chain reaction technique and the protein truncation test. 1996

Romey, M.C., Tuffery, S., Desgeorges, M., Bienvenu, T., Demaille, J. and Claustres, M.

Notes: RT-PCR and PTT detected truncated peptides produced from the mRNA transcripts of mutant CFTR genes. Due to the large size of the gene for CFTR, RT-PCR and the PTT are attractive tests for screening for frameshift mutations in the gene. The PTT was performed using an aliquot of nested PCR product (consisting of the CFTR cDNA fused to the T7-promoter) in the TNT® T7 Coupled Reticulocyte Lysate System. (1869)

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Proc. Natl. Acad. Sci. USA 93, 12845-12850. Transcriptional repression by YY1 is mediated by interaction with a mammalian homolog of the yeast global regulator RPD3. 1996

Yang, W. M. , Inouye, C. , Zeng, Y. , Bearss, D. , Seto, E.

Notes: The pGEM®-7zf(-) vector was used for subcloning cDNAs. The pCAT®3-Control Vector was used as a control in studies of CAT reporter constructs in CV1 and HeLa cells. GST and GST-YY1 fusion proteins were produced using the TNT® T7 Coupled Reticulocyte Lysate System. (0141)

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Cell 86, 391-399. XTcf-3 transcription factor mediates beta-catenin-induced axis formation in Xenopus embryos. 1996

Molenaar, M., van de Wetering, M., Oosterwegel, M., Peterson Maduro, J., Godsave, S., Korinek, V., Roose, J., Destree, O., Clevers, H.

Notes: XTcf-3, β-catenin and their derivatives were transcribed and translated in the T7 TNT® Coupled Reticulocyte Lysate System. Gel retardation assays were performed with dsDNA probe from the T cell receptor a enhancer. The β-catenin and DN/C/beta-catenin yielded supershifted bands. Deletion of the first 31 amino acids abrogated the interaction with beta-catenin. (0655)

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Nucl. Acids Res. 23, 2555-2562. Xenopus sonic hedgehog as a potential morphogen during embryogenesis and thyroid hormone-dependent metamorphosis. 1995

Stolow, M. A., Shi, Y. B.

Notes: Xenopus sonic hedgehog (XHH) cDNA clones were transcribed and translated using the TNT® T7 Coupled Reticulocyte Lysate System. Two smaller variants were found. The smaller proteins may represent proteolytic products. (0338)

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