Tumor-associated zinc finger mutations in the CTCF transcription factor
selectively alter its DNA-binding specificity.
Filippova, G.N., Qi, C-F., Ulmer, J.E., Moore, J.M., Ward, M.D., Hu, Y.J., Loukinov, D.I., Pugacheva, E.M., Klenova, E.M., Grundy, P.E., Feinberg, A.P., Cleton-Jansen, A-M., Moerland, E.W., Cornelisse, C.J., Suzuki, H., Komiya, A., Lindblom, A., Dorion-Bonnet, F., Neiman, P.E., Morse, H.C., Collins, S.J. and Lobanenkov V.V.
Notes: In this study, mutations in the CTCF zinc finger transcription factor were found by screening tumor samples from cancer patients. These same mutations were introduced into a cloned version of the gene and the encoded proteins were translated in vitro using the TNT® T7 Coupled Rabbit Reticulocyte Lysate System. The translated proteins were used in gel shift assays with radiolabeled CTCF target DNA. The gel-shift binding buffer was supplemented with 0.1mM zinc sulfate. (3133)
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