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Infect. Immun. 70, 1984-1990. Helicobacter pylori uses motility for initial colonization and to attain robust infection. 2002

Ottemann, K.M. and Lowenthal, A.C.

Notes: Genomic DNA was isolated from H. pylori with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used in both PCR amplification and Southern blotting. (2292)

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Cell 109, 257-266. Asymmetric cell division in B. subtilis involves a spiral-like intermediate of the cytokinetic protein FtsZ. 2002

Ben-Yehuda, S. and Losick, R.

Notes: In this study, the Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Bacillus subtilis. (2428)

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Infect. Immun. 70, 4389–4398. Characterization of Pit, a Streptococcus pneumoniae iron uptake ABC transporter. 2002

Brown, J.S., Gilliland, S.M., Ruiz-Albert, J. and Holden, D.W.

Notes: The authors describe using the Wizard® Genomic DNA Purification kit and the SV Total RNA Isolation System to isolate genomic DNA and total RNA, respectively, from Streptococcus pneumoniae. The researchers also added RNasin® Ribonuclease Inhibitor to purified total RNA before storing it and using it for later RT-PCR reactions performed with the Access RT-PCR System. (2835)

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Genetics 160, 225-234. Cloning and characterization of the Tribolium cataneum eye-color genes encoding tryptophan oxygenase and kynurenine 3-monooxygenase 2002

Lorenzen, M.D., Brown, S.J., Dennell, R.E., and Beeman, R.W.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from individual Tribolium cataneum flour beetles. The isolated genomic DNA was used for PCR amplification for recombinational mapping related to eye color, PCR-based deletion breakpoint analysis, and was EcoR I-digested for Southern blotting analysis. (2504)

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Science 295, 483-485. Electrode-reducing microorganisms that harvest energy from marine sediments 2002

Bond, D.R., Holmes, D.E., Tender, L.M., Lovley, D.R.

Notes: The authors report an enrichment of microorganisms belonging to the Geobacteraceae family on energy-harvesting anodes from marine sediments. Electrodes were rinsed under sterile marine buffer then scraped with sterile razor blades to remove microorganisms. DNA was extracted using an organic extraction method and then further purified using a Wizard® Genomic DNA Purification Kit. (2592)

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Antimicrob. Agents Chemother. 46, 615-624. Molecular evaluation of the plasma membrane proton pump from Aspergillus fumigatus. 2002

Burghoorn, H.P., Soteropoulos, P., Paderu, P., Kashiwazaki, R., and Perlin, D.S.

Notes: The Wizard® Genomic DNA Purification Kit was used to purify DNA from two strains of Aspergillus fumigatus.  A modified protocol was used for the purification.  Cells were filtered through a 0.2μm SFCA filter, frozen on dry ice and then ground with a mortar and pestle.  The ground cells were then incubated  in Nuclei Lysis Solution at 37°C for 1 h before continuing with the Wizard® Genomic DNA Tissue Culture Cell procedure.  The purified DNA was used in PCR with primers for the A. fumigatus PMA1 gene. (3048)

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Infect. Immun. 70, 323-334. Novel 45-kilodalton leptospiral protein that is processed to a 31-kilodalton growth-phase-regulated peripheral membrane protein. 2002

Matsunaga, J., Young, T.A., Barnett, J.K., Barnett, D., Bolin, C.A. and Haake, D.A.

Notes: Genomic DNA was isolated from Leptospira kirschneri with the Wizard® Genomic DNA Purification Kit.  The isolated DNA was used for Southern blotting. (2296)

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Infect. Immun. 70, 481-490. Role of the dermonecrotic toxin of Bordetella bronchiseptica in the pathogenesis of respiratory disease in swine. 2002

Brockmeier, S.L., Register, K.B., Magyar, T., Lax, A.J., Pullinger, G.D., and Kunkle, R.A.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from wildtype and engineered mutants of B. bronchiseptica. The isolated genomic DNA was used in PCR to analyze the size of the dnt gene. The engineered deletion mutants produced a 1.7kb PCR product and the wildtype gene produced a 2.3kb product. (2293)

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EMBO J. 20, 1164-1172. Mutations in DnaA protein suppress the growth arrest of acidic phospholipid-deficient Eschericia coli cells. 2001

Zheng, W., Li, Z., Skarstad, K. and Crooke, E.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from 5ml exponentially growing E. coli cultures.  The isolated DNA was used for Southern blotting. (2298)

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Appl. Environ. Microbiol. 67, 4225-4232. Photoreactivation in airborne Mycobacterium parafortuitum. 2001

Peccia, J. and Hernandez, M.

Notes: Genomic DNA was isolated from Serratia marcescens and Mycobacterium parafortuitum with the Wizard® Genomic DNA Purification Kit.  Isolation from Serratia was straightforward following the recommended protocol in Technical Manual TM050.  Mycobacterium cells were first treated with proteinase K and lysozyme followed by addition of glass beads. After vigorous shaking in a bead beater, genomic DNA was isolated using the standard protocol from then on. The isolated DNA was quantitated by a DAPI-based quantitation for irradiated and nonirradiated DNA. (2290)

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J. Bacteriol. 183, 7094-7101. Quantification of expression of Staphylococcus epidermidis housekeeping genes with Taqman quantitative PCR during in vitro growth and under different conditions. 2001

Vandecasteele, S.J., Peetermans, W.E., Merckx, R. and Van Eldere, J.

Notes: M-MLV Reverse Transcriptase and RNasin® Ribonuclease Inhibitor were used in a reaction to generate first strand cDNA.  The generated cDNA was used as a template for quantitative PCR in a Taqman® Assay.  The pGEM®-T Vector System was used to clone copies of each target gene so that standards could be generated.  The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from the S. epidermidis to generate a standard curve for quantitation of genomic DNA contamination of samples. (2291)

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Appl. Environ. Microbiol. 67, 3295-3298. Rapid approach to determine rrn arrangement in Salmonella serovars. 2001

Helm, R.A. and Maloy, S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from Salmonella enterica serovars.  The isolated DNA was used as template in PCR reactions with 49 primer sets to examine the structure of the rrn operon. Some amplimers were up to 7kb in size. (2297)

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EMBO J. 20, 3229-3237. Telomere resolution in the Lyme disease spirochete. 2001

Chaconas, G., Stewart, P.E., Tilly, K., Bono, J.L., and Rosa, P.

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from 5ml early stationary phase cultures of Borrelia burgdorferi.  Isolated genomic DNA was used in both Southern blotting and PCR. (2294)

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Genome Res. 11, 1706-1715. Whole genome comparison of Campylobacter jejuni human isolates using a low-cost microarray reveals extensive genetic diversity. 2001

Dorrell, N., Mangan, J.A., Laing, K.G., Hinds, J., Linton, D., Al-Ghusein, H., Barrell, B.G., Parkhill, J., Stoker, N.G., Karlyshev, A.V., Butcher, P.D., and Wren, B.W.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate target genomic DNA from C. jejuni human isolates for microarray analysis.  The isolated DNA was used as a template for Cy®3- and Cy®5-dCTP incorporation by random primed labeling reactions with Klenow. (2295)

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J. Biol. Chem. 275(33), 25690-25699. A Novel Human Gene Similar to the Protein Kinase (PK) Coding Domain of the Large Subunit of Herpes Simplex Virus Type 2 Ribonucleotide Reductase (ICP10) Codes for a Serine-Threonine PK and Is Expressed in Melanoma Cells 2000

Smith, C.C., Yu, Y.X., Kulka, M., and Aurelian, L.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from 5 x 107 human G361 melanoma cells. The isolated DNA was used for Southern blotting. Probes were constructed with the DNA 5'-End Labeling System. The cDNA for the H11 protein was expressed in 293 HEK cells using the pCI Mammalian Expression Vector. (0075)

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Appl. Environ. Microbiol. 66, 671-677. Cytochrome c(3) mutants of Desulfovibrio desulfuricans 2000

Rapp-Giles, B.J., Casalot, L., English, R.S., Ringbauer, J.A.Jr., Dolla., A., Wall, J.D.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from D. desulfuricans. The isolated DNA was used for Southern analysis and cosmid library construction. (0499)

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J. Biol. Chem. 275, 3343-3347. Effects of aging on mitochondrial DNA copy number and cytochrome c oxidase gene expression in rat skeletal muscle, liver and heart 2000

Barazzoni, R., Short, K.R. and Nair, K.S.

Notes: In this paper, the Wizard® Genomic DNA Purification Kit was used to isolate total DNA from various tissues including rat skeletal muscle, liver and heart. Purification was performed with 30-50mg of tissue. The isolated tissue was used for Southern analysis of mitochondrial DNA sequences. (2233)

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J. Bacteriol. 182, 1356-1363. Gene families encoding phase- and size-variable surface lipoproteins of Mycoplasma hyorhinis. 2000

Citti, C., Watson-McKown, R., Droesse, M., Wise, K.S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolate genomic DNA from Mycoplasma hyorhinis. The isolated DNA was used for PCR. (1306)

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Appl. Environ. Microbiol. 66, 1796–1800. In situ reverse transcription-PCR for monitoring gene expression in individual Methanosarcina mazei S-6 Cells. 2000

Lange, M., Tolker-Nielsen, T., Molin, S. and Ahring, B.K.

Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from the archaeabacterium, Methanosarcina mazei S-6.  The isolated genomic DNA was used in PCR to create templates for sequencing reactions.  (3106)

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Infect. Immun. 68, 7180-7185. Phylogeny of Vibrio cholerae based on recA sequences. 2000

Stine, O.C., Sozhamannan, S., Gou, Q., Zheng, S., Morris, J.G., Jr., and Johnson, J.A.

Notes: Chromosomal DNA was isolated from V. cholerae with the Wizard® Genomic DNA Purification Kit prior to PCR.  The PCR products were directly purified with the Wizard® PCR Preps System.  The purified 788bp amplimers were used directly for fluorescent Big Dye sequencing. (2299)

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J. Clin. Microbiol. 38(8), 2829-2836. Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon 2000

Park, S., Wong, M., Marras, S. A. E., Cross, E.W., Kiehn, T.E., Chaturvedi, V., Tyagi, S., and Perlin, D.S.

Notes: Various Candida sp. were treated with a zymolase solution then genomic DNA was isolated with the Wizard® Genomic DNA Purification Kit. The isolated DNA was used for RAPD analysis with Promega's Taq DNA Polymerase, 10X Thermophilic Reaction Buffer and Nuclease-Free Water. Further diagnostic procedures used Promega's dNTP's and Promega's PCR Nucleotide Mix. (0074)

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Genetics 155, 1149-1160. RNA editing of the Drosophila para Na+ channel transcript: Evolutionary conservation and developmental regulation. 2000

Hanrahan, C.J., Palladino, M.J., Ganetsky, B. and Reenan, R.A.

Notes: Genomic DNA was extracted from 100 Drosophila melanogaster and 100 Drosophila virilis using the Wizard® Genomic DNA Purification Kit.  The isolated genomic DNA was used for PCR amplification and direct sequencing.  RT-PCR was also performed in the presence of RNasin® Ribonuclease Inhibitor and cloned amplimers were purified with the Wizard® Plus Minipreps DNA Purification System prior to fluorescent sequencing. (2505)

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Proc. Natl. Acad. Sci. USA 96, 2285-2290. Activation of target-tissue immune-recognition molecules by double-stranded polynucleotides. 1999

Suzuki, K., Mori, A., Ishii, K.J., Saito, J., Singer, D.S., Klinman, D.M., Krause, P.R. and Kohn, L.D.

Notes: The authors used the Wizard® Genomic DNA Purification Kit to isolate genomic DNA from rat FRTL-5 thyroid cells. (0282)

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J. Bacteriol. 181, 6907-6913. Anaerobic growth of Paracoccus denitrificans requires cobalamin: Characterization of cobK and cobJ genes. 1999

Shearer, N., Hinsley, A.P., Van Spanning, R.J.M., Spiro, S.

Notes: The Wizard® Genomic DNA Purification Kit was used to isolated genomic DNA from P. denitrificans. The isolated DNA was used for Southern analysis. (0386)

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J. Immunol. 162, 1566-1572. Atypical VH-D-JH rearrangements in newborn autoimmune MRL mice. 1999

Klonowski, K.D., Primiano, L.L., Monestier, M.

Notes: Authors use the Wizard® Genomic DNA Purification Kit to isolate DNA from newborn mice livers. They performed nested PCR on V(H)-D-J(H) genes and cloned these into the pGEM®-T Vector and sequenced the rearrangements using the fmol® DNA Cycle Sequencing System. (0908)

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