Li, Y., Pan, Y., Qi, F., and Caufield, P.W.
Notes: The Wizard® Genomic DNA Purification Kit was used to purify genomic DNA from various Streptococcal strains (Streptococcus sanguinis, S. oralis, S. gordonii, S. cristatus, S. mitis, S. parasanguinis, S. pneumoniae, S. mutans, S. salivarius, S. sobrinus, S. ratti, and S. vestibularis), Lactobacillus acidophilus, Actinomyces naeslundii, and E. coli JM109. The genomic DNA was diluted to 50μg/ml and used in Arbitrarily Primed PCR (AP-PCR) experiments to identify common amplimers from clinical Streptococcus sanguinis samples. The authors were able to identify a specific probe that may be helpful in identification of S. sanguinis clinical isolates. (2836)