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Anaerobe 18, 566–75. Morphological, biochemical, physiological and molecular aspects of the response of Fusobacterium nucleatum exposed to subinhibitory concentrations of antimicrobials. 2012

de Souza Filho, J.A. et al.

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Fusobacterium nucleatum. (4335)

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Plasmid 68, 1–12. Requirements for Borrelia burgdorferi plasmid maintenance. 2012

Tilly, K., Checroun, C. and Rosa, P.A.

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Borrelia burgdorferi. (4315)

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J. Clin. Microbiol. 50(5), 1580–5. Sensitive and rapid detection of the New Delhi metallo-beta-lactamase gene by loop-mediated isothermal amplification. 2012

Liu, W. et al.

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Acinetobacter baumannii. (4274)

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Appl. Environ. Microbiol. 78, 6121–7. The physiological opportunism of Desulfitobacterium hafniense strain TCE1 towards organohalide respiration with tetrachloroethene. 2012

Duret, A., Holliger, C. and Maillard J.

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Desulfitobacterium hafniense. (4326)

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Vet. Parasitol. 188, 160–3. Use of a real time PCR for detecting subspecies of Babesia canis. 2012

Costa, L.M., Jr. et al.

Notes: In this study the Wizard® Genomic DNA Purification Kit was used to isolate DNA from Babesia canis. (4311)

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Food Control 23, 400-404. Development and validation of fast Real-Time PCR assays for species identification in raw and cooked meat mixtures. 2012

Cammà, C., Di Domenico, M., and Monaco, F.

Notes: These authors used the Maxwell® 16 Tissue DNA Purification Kit to extract DNA from 200µl samples of raw meat homogenate from beef, pork, poultry and lamb samples. They also used the Wizard® Genomic DNA Isolation System to extract DNA from cooked meat samples. The extracted DNA was used in real-time, quantitative PCR assays to identify species-specific DNA spiked at 1% in mixed DNA samples. (4353)

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J. Microbiol. Methods 81, 127-134. Comparative analysis of fecal DNA extraction methods with phylogenetic microarray: effective recovery of bacterial and archaeal DNA using mechanical cell lysis. 2011

Salonen, A., Nikkilä, J., Jalanka-Tuovinen, J., Immonen, O., Rajilić-Stojanović, M., Kekkonen, R.A., Palva, A., and de Vos, W.M.

Notes: These authors compared the performance of four DNA purification methods for recovery of bacterial and archaeal DNA from fecal material. One of the methods tested was the Wizard® Genomic DNA Purification Kit, which uses a solution-based, enzymatic method for extraction. The Wizard® Genomic method was rated highly on extraction speed, and gave the highest DNA yields. A second method involving mechanical disruption (repeated bead beating) was rated more highly on extraction efficiency from archaea and some bacterial species. The criteria for performance comparison are described fully in the paper. (4219)

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Arch. Med. Sci. 7, 501-507. Frequency of Firmicutes and Bacteroidetes in gut microbiota in obese and normal weight Egyptian children and adults. 2011

Ismail, N.A., Ragab, S.H., Elbaky, A.A, Shoeib, A.R., Alhosary, Y., and Fekry, D.

Notes: These authors investigated the differences in gut microbial flora between obese and normal-weight subjects. They used the Wizard® Genomic DNA Purification Kit to extract DNA from diluted fecal extracts. The extracted DNA was analyzed by PCR to identify Bacteroidetes and Firmicutes. Differences in distribution of these phyla was between the subject groups were identified. (4220)

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Appl. Environ. Microbiol. 76, 829–42. Lysogeny and sporulation in Bacillus isolates from the Gulf of Mexico. 2010

Mobberley, J., Authement, R.N., Segall, A.M., Edwards, R.A., Slepecky, R.A. and Paul, J.H.

Notes: Certain bacteriophages can promote host cell sporulation under unfavorable conditions to increase survival of the host and prophage. These types of phages, known as spore-converting phages, have been found in terrestrial Bacillus species. In this article the authors examined the effect of prophages on sporulation of 11 Bacillus isolates from the Gulf of Mexico. Potential prophages in the Bacillus isolates were detected by PCR using unique PCR primer sets for each prophage genome and GoTaq® Green Master Mix. One of these isolates, B14905, was examined in more detail; the genome of this isolate was isolated using the Wizard® Genomic DNA Purification Kit, then sequenced. (4091)

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Nucl. Acids Res. 37, 7468–7482. Enhanced gene repair mediated by methyl-CpG-modified single-stranded oligonucleotides. 2009

Bertoni, C., Rustagi, A. and Rando, T.A.

Notes: This paper explored the effect of methyl-CpGmodified single-stranded oligonucleotides (ssODN) on gene repair. The Wizard® Genomic DNA Purification Kit was used to isolate gDNA from methylCpG-ssODN-treated myoblasts derived from limb muscle of neonatal C57 mice that stably expressed the mismatch repair site. One microgram of purified gDNA was digested overnight with 5U of restriction enzyme, purified, resuspended in 20µl of water and 5µl used in real-time PCR to determine if the target had been repaired. (4062)

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Proc. Natl. Acad. Sci. USA 106, 12279–12282. Genetic identification of putative remains of the famous astronomer Nicolaus Copernicus. 2009

Bogdanowicz, W., Allen, M., Branicki, W., Lembring, M., Gajewska, M. and Kupiec, T.

Notes: The authors were interested on confirming the identity of the exhumed skeletal remains thought to be Nicolaus Copernicus. DNA isolation from teeth was performed by pulverizing the tooth in liquid nitrogen, soaking the powdered sample in 0.5M EDTA, 5% SDS and 3 mg proteinase K at 37 °C and extracting genomic DNA using the Wizard® Genomic DNA Purification Kit. Bone and other tooth samples were extracted using another method and the Wizard® Genomic DNA Purification Kit used for a salting-out method. Purified DNA was subjected to mitochondrial, autosomal and Y chromosome STR amplification and analysis. (4063)

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J. Gen. Virol. 90, 1461–1470. Murid herpesvirus-4 lacking thymidine kinase reveals route-dependent requirements for host colonization. 2009

Gill, M.B., Wright, D.E., Smith, C.M., May, J.S. and Stevenson, P.G.

Notes: The authors examined the role of thymidine kinase (TK) in establishing a herpesvirus infection via the upper respiratory tract. DNA was purified from ex vivo organs of female BALB/c mice infected with a murid herpesvirus-4 (MuHV-4) TK knockout using the Wizard® Genomic DNA Purification Kit. Real-time PCR was used with 50–80ng of purified DNA to determine viral load of the animals. (4015)

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Nucl. Acids Res. 37, e9. Mutagenic inverted repeat assisted genome engineering (MIRAGE). 2009

Nair, N.U. and Zhao, H.

Notes: In this paper, the researchers describe and demonstrate a new method for creating precise genome modifications in Saccharomyces cerevisiae. The mutagenic inverted repeat assisted genome engineering (MIRAGE) was tested in S. cerevisiae W303a by deleting gal7 as well as point and frameshift mutations. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit, amplified and modifications verified by gel analysis or DNA sequencing. (4014)

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Vet. Rec. 164, 44–47. Prevalence of thermophilic Campylobacter species in household cats and dogs in Ireland. 2009

Acke, E., McGill, K., Golden, O., Jones, B.R., Fanning, S. and Whyte, P.

Notes: To examine the prevalence of Campylobacter species in asymtomatic carriers that can pass the bacteria onto humans, rectal swabs were collected from 147 dogs and 35 cats in Ireland and cultured on various diagnostic plates. The Wizard® Genomic DNA Purification System was used to isolate DNA from any suspect Campylobacter cultures. The purified DNA was used in multiplex PCR and RFLP to determine which species of Campylobacter was present. (4016)

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Nucl. Acids Res. 37, 1951–1961. Reactive oxygen species generated by thiopurine/UVA cause irreparable transcription-blocking DNA lesions. 2009

Brem, R., Li, F. and Karran, P.

Notes: The association of thiopurines (anticancer drugs) with acute skin sensitivity to ultraviolet A (UVA) radiation and a high risk of skin cancer was tested using six human fibroblast and lymphoid cell lines. The thiopurine 6-thioguanine (6-TG) was added at 0.8 or 0.6mM to each of six cell lines and incubated for 48 hours to ensure incorporation. DNA and RNA were extracted and 40µg of nucleic acid were digested to nucleosides, separated by HPLC, and the 6-TG 20-deoxy and ribonucleosides quantified by absorbance at 342 nm. The same DNA isolation and digestion method was used when the cells were treated with 1µM of 6-TG and then irradiated with 5 kJ/m2 UVA after 48 hours. The Wizard® Genomic DNA Purification Kit was used for DNA extraction. (4013)

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Clin. Can. Res. 14(19), 6062–72. Androgen-regulated and highly tumorigenic human prostate cancer cell line established from a transplantable primary CWR22 tumor. 2008

Dagvadorj, A., Tan, S.H., Liao, Z., Cavalli, L.R., Haddad, B.R. and Nevalainen, M.T.

Notes: The authors developed a new human prostate cancer cell line, CWR22Pc, that is both androgen-dependent and able to produce tumors in dihydrotestosterone-supplemented nude mice. To confirm that CWR22Pc cells are derived from primary CWR22 human prostate xenograft tumors, the authors performed genotyping at 8 STR loci and amelogenin using the PowerPlex® 1.2 System. DNA purification from the cell line and original tumor samples was performed using the Wizard® Genomic DNA Purification Kit. (4041)

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Appl. Environ. Microbiol. 74, 312-318. Characterization of two new genes, amoR and amoD, in the amo operon of the marine ammonia oxidizer Nitrosococcus oceani ATCC 19707. 2008

El Sheikh, A.F., Poret-Peterson, A.T. and Klotz, M.G.

Notes: These authors investigated the amo operon of the marine ammonia oxidizer Nitrosococcus oceani. The bacteria were grown at 30°C for 3 weeks in 200-400ml batch cultures in artificial seawater in the dark without shaking. Genomic DNA was isolated from cells in stationary phase using the Wizard® Genomic DNA Purification Kit. The isolated DNA was then used for PCR analysis. (3740)

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Plant Physiol. 148, 479–489. Eukaryotic translation initiation factor 5A is involved in pathogen-induced cell death and development of disease. 2008

Hopkins, M.T., Lampi, Y., Wang, T.W., Liu, Z. and Thompson, J.E.

Notes: Genomic DNA from Arabidopsis thaliana was isolated using the Wizard® Genomic DNA Purification Kit. The extracted DNA was used to amplify the 3´ UTR of AteIF5A-2, A. thaliana translation initiation factor 5A, or the entire gene for creating transgenic plants. Leaf discs from wild-type Arabidopsis were infected with Pseudomonas syringae pv tomato DC3000, a virulent strain regulated by AteIF5A-2, using a syringe. After 24 and 72 hours, 0.4cm leaf discs were fixed, labeled using the DeadEnd™ Fluorometric TUNEL System and stained for AteIF5A-2 protein. (3979)

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J. Clin. Microbiol. 46, 1741–1746. High-throughput genotyping of Salmonella enterica serovar Typhi allowing geographical assignment of haplotypes and and pathotypes within an urban District of Jakarta, Indonesia. 2008

Baker, S., Holt, K., van de Vosse, E., Roumagnac, P., Whitehead, S., King, E., Ewels, P., Keniry, A., Weill, F.X., Lightfoot, D., van Dissel, J.T., Sanderson, K.E., Farrar, J., Achtman, M., Deloukas, P. and Dougan, G.

Notes: The authors examined strains of Salmonella enterica serovar Typhi isolated from typhoid cases originating in or around Indonesia or from travelers returning from Indonesia to examine if serovar Typhi from this area has a greater level of genetic diversity compared to other countries. Genomic DNA was isolated using the Wizard® Genomic DNA Purification Kit, diluted to 4 ng/µl and used in locus-specific PCR genotyping. (3980)

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Proc. Natl. Acad. Sci. USA 105, 7141-7146. Lack of aldose 1-epimerase in Hypocrea jecorina (anamorph Trichoderma reesei): A key to cellulase gene expression on lactose 2008

Fekete, E., Seiboth, B., Kubicek, C.P., Szentirmai, A., Karaffa, L.

Notes: To amplify yeast mutarotase, S. cerevisiae was used, and E. coli strain JM109 (Promega Cat.# L2001) was used for plasmid propagation. Fungal mycelia were harvested by filtration, washed, frozen and ground under liquid nitrogen. Genomic DNA was extracted using the Wizard Genomic DNA Purification System (Promega Cat.# A1120). RNA for hybridization and RT-PCR was extracted from mycelia using the SV Total RNA Isolation System (Promega Cat.# Z3101) and plasmid DNA isolated using the PureYield(TM) Plasmid Midiprep System (Cat.# A2492). (3919)

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J. Biol. Chem. 283, 18158–18166. MicroRNA miR-199a* regulates the MET proto-oncogene and the downstream extracellular signal-regulated kinase 2. 2008

Kim, S., Lee, U.J., Kim, M.N., Lee, E.J., Kim, J.Y., Lee, M.Y., Choung, S., Kim, Y.J. and Choi, Y.C.

Notes: To examine the methylation state of DNA of cultured cells, genomic DNA was purified using the Wizard® Genomic DNA Purification Kit. The extracted DNA was restriction enzyme digested and then treated with bisulfite. (3978)

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Int. Congr. Ser. 1239, 207–12. Population data on Powerplex 2.1 (FGA, vWA, TPOX, THO1, Penta E, D18S51, D21S11, D3S1358, D8S1179) and Gammastar (D16S539, D7S820, D13S317, D5S818) in a sample of Caucasian-Mestizos from Colombia. 2008

Yunis, J.J., García, O., Moreno, S., Pineda, C., Rodriguez, C., Uriarte, I. and Yunis, E.J.

Notes: The authors generated population data for unrelated Caucasian-Mestizos from Columbia using the PowerPlex® 2.1 System and the GammaSTR® Multiplex. DNA was isolated from whole blood using the Wizard® Genomic DNA Purification Kit or ReadyAmp™ Genomic DNA Purification System, then amplified per the manufacturer's recommendations. Amplified fragments were detected using a Hitachi FMBIO® II fluorescence imaging system. (3856)

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J. Bacteriol. 190, 1649–1657. Structural and biological characterization of a capsular polysaccharide produced by Staphylococcus haemolyticus. 2008

Flahaut, S., Vinogradov, E., Kelley, K.A., Brennan, S., Hiramatsu, K. and Lee, J.C.

Notes: The authors wanted to purify and characterize the capsular polysaccharide (CP) produced by Staphylococcus haemolyticus strain JCSC1435. S. haemolyticus strains grown in TSB cultures were harvested, lysed with lysozyme and lysostaphin and genomic DNA isolated using the Wizard® Genomic DNA Purification Kit. The DNA was then used in CP gene PCR. Total RNA was isolated from exponential, postexponential, and stationary phases of S. haemolyticus growth and used in RT-PCR using the Access RT-PCR System. (3977)

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Appl. Environ. Microbiol. 74, 811-817. High frequency of histamine-producing bacteria in enological environment and instability of the phenotype. 2007

Lucas, P.M., Claisse, O. and Lonvaud-Funel, A.

Notes: Because of concerns about the consumption of histamine in wine (which makes histamine more potent), the quantity of lactic acid bacteria (LAB), which can produce histamine during winemaking, was determined in 264 samples of red wine from 116 wineries. DNA was isolated from LAB strains grown in De Man Rogosa Sharpe (MRS) broth using the Wizard® Genomic DNA Purification Kit. Glass beads were used to disrupt the cells, and a modified Wizard® Genomic DNA Purification protocol that included PVP treatment was performed. The purified DNA was used in qPCR analysis. (3741)

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Emerging Infect. Dis. 13, 1756-1758. Human Bocavirus infection in children with gastroenteritis, Brazil. 2007

Albuquerque, M.C., Rocha, L.N., Benati, F.J., Soares, C.C., Maranhão, A.G., Ramírez, M.L, Erdman, D., and Santos, N.

Notes: In this study, the Wizard® Genomic DNA Purification Kit was used to extract DNA from diluted fecal samples. The extracted DNA was used in PCR with specific primers to detect viral sequences. PCR fragments were gel-purified using the SV Gel and PCR Clean-Up System prior to sequencing to confirm the Bocavirus DNA identity. (4221)

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