Ito, M., Jameson, J.L., Ito, M.
Notes: Mutant and wildtype arginine vasopressin (AVP) constructs were co-transfected with a CAT-expression vector containing a neo resistance gene. Stable transformants of Neuro2A cells were generated with the aid of the Transfectam® Reagent and G418 sulfate. CAT activity in stably transfected clones was determined with the CAT Enzyme Assay System. The effect of the mutant AVP constructs on cell viability was determined with the CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS). Several constructs displayed marked decreases in cell viability. The cause was necrosis rather than apoptosis due to the absence of DNA laddering and a negative reaction in the Apoptosis Detection System, Fluoroscein (data not shown). The name of the Apoptosis Detection System, Fluorescein, has changed to DeadEnd™ Fluorometric TUNEL System. (1009)