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J. Pharmacol. Exp. Ther. 286, 366-380. Pharmacology and intracellular signaling mechanisms of the native human orphan receptor BRS-3 in lung cancer cells 1998

Ryan, R.R., Weber, H.C., Mantey, S.A., How, W., Hilburger, M.E., Pradhan, T.K., Coy, D.H., Jensen, R.T.

Notes: The ability of hyuman bombesin receptor subtype 3 (hBRS-3) to stimulate cell proliferation in the human lung cancer cell line, NCI-N417 was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2532)

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Am. J. Physiol. 275, c1216-c1223. Regulation of angiotensin II-induced JAK2 tyrosine phosphorylation: Roles of SHP-1 and SHP-2 1998

Marrero, M.B., Venema, V.J., Ju, H., Eaton, D.C., Venema, R.C.

Notes: The effect of anti-SHP-2 antibody electroporation on ANG II-induced vascular smooth muscle cell proliferation was determined using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2552)

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J. Clin. Invest. 102, 653-662. Retinoic acid uses divergent mechanisms to activate or suppress mitogenesis in rat aortic smooth muscle cells 1998

Chen, S., Gardner, D.G.

Notes: Rat aortic smooth muscle cells  were cultured in 96-well plates and growth arrested. The quiescent cells were treated with a variety of concentrations of all-trans retinoic acid with our without endothelin. Cell proliferation of the cells was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2551)

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Mol. Cell. Biol. 18, 6245-6252. Synergistic regulation of Schwann cell proliferation by heregulin and forskolin 1998

Rahmatullah, M., Schroering, A., Rothblum, K., Stahl, R.C., Urban, B., Carey, D.J.

Notes: Schwann cells were incubated for 6 days in the absence or presence of heregulin peptide, either with or without forskolin, and cell proliferation was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2542)

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J. Biol. Chem. 273, 25987-25995. The CC chemokine monocyte chemotactic peptide-1 activates both the class I p85/p110 phosphatidylinositol 3-kinase and the class II PI3K-C2α 1998

Turner, S.J., Domin, J., Watefield, M.D., Ward, S.G., Westwick, J.

Notes: Chemotaxis in THP-1 human monocytic cells expressing the chemokine receptor 2 was examined using a 96-well chemotaxis chamber. Cell migration was assessed by using the CellTiter 96® AQueous Non-Radioactive Assay. (2544)

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Cell Death Differ. 5, 381-389. The growth arrest and downregulation of c-myc transcription induced by ceramide are related events dependent on p21 induction, Rb underphosphorylation and E2F sequestering. 1998

Alesse, E., Zazzeroni, F., Angelucci, A., Giannini, G., Di Marcotullio, L. and Gulino, A.

Notes: The CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS) was used to monitor cell viability of the Hs27 human diploid fibroblast cell line following treatment with ceramide. (1507)

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Am. J. Pathol. 153, 381-394. Vascular endothelial growth factor-C (VEGF-C/VEGF-2) promotes angiogenesis in the setting of tissue ischemia. 1998

Witzenbichler, B., Asahara, T., Murohara, T., Silver, M., Spyridopoulos, I., Magner, M., Principe, N., Kearney, M., Hu, J.-S., Isner, J.M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to monitor the proliferative response of human umbilical vein endothelial cells to VEGF-C. RT-PCR was performed in the presence of RNasin® Ribonuclease Inhibitor. (0146)

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J. Neurosci. 17, 6114-6121. Apolipoprotein E binds to and potentiates the biological activity of ciliary neurotrophic factor. 1997

Gutman, C. R. , Strittmatter, W. J. , Weisgraber, K. H. , Matthew, W. D.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to assess the viability of rat embryonic hippocampal neurons 72 hours after treatment with apoE and CNTF. (1191)

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Proc. Natl. Acad. Sci. USA 94, 10907-10912. Association of arsenic-induced malignant transformation with DNA hypomethylation and aberrant gene expression 1997

Zhao, C.Q., Young, M.R., Diwan, B.A., Coogan, T.P., Waalkes, M.P.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to quantify the cytotoxicity of sodium arsenite in TRL 1215 cells. (2547)

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J. Immunol. 159(8), 4024-4034. Attenuation of IL-5-mediated signal transduction, eosinophil survival, and inflammatory mediator release by a soluble human IL-5 receptor. 1997

Monohan, J., Siegel, N., Keith, R., Caparon, M., Christine, L., Compton, R., Cusik, S., Hirsch, J., Huynh, M., Devine, C., Polazzi, J., Rangwala, S., Tsai, B. and Portanova, J.

Notes: The MTS-based CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to measure the effects of soluble IL-5Ra or anti-IL-5 mAb (TRFK-5) on survial of human eosinophils. (2077)

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J. Biol. Chem. 272(30), 18827-18833. Characterization of FAS (Apo-1, CD95)-Fas ligand interaction 1997

Schneider, P., Bodmer, J-L., Holler, N., Mattmann, C., Scuderi, P, Terskikh, A., Peitsch, M., and Tschopp, J.

Notes: The CellTiter 96® AQueous Assay (MTS Reagent) was used to measure the cytotoxic activity of wildtype and mutant soluble Fas ligand (sFasL) on murine B lymphoma A20 cells and human T lymphoblastoma Jurkat cells. (1706)

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J. Biol. Chem. 272(30), 18827-18833. Characterization of Fas (Apo-1, CD95)-Fas ligand interaction. 1997

Schneider, P., Bodmer, J.L., Holler, N., Mattmann, C., Scuderi, P., Terskikh, A., Peitsch, M.C., Tschopp, J.

Notes: The CellTiter 96® AQueous Assay (MTS reagent) was used to measure the cytotoxic activity of wild type and mutant soluble Fas ligand (sFasL) on murine B lymphoma A20 cells and human T lymphoblastoma Jurkatt cells. (2861)

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Blood 90, 3456-3461. Chronic thrombocytopenia is induced in dogs by development of cross- reacting antibodies to the MpL ligand 1997

Dale, D. C. , Nichol, J. L. , Rich, D. A. , Best, D. M. , Slichter, S. J. , Sheridan, W. , Hunt, P.

Notes: The CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS) was used to measure the IC50 for anti-MpL ligand. Murine 32D cells that were transfected with the human MpL receptor. (1251)

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Stem Cells 15, 275-285. Coexpression of Kit and the receptors for erythropoietin, interleukin 6 and GM-CSF on hemopoietic cells 1997

de Jong, M.O., Westerman, Y., Wagemaker, G., Wognum, A.W.

Notes: Various growth factors were labeled with digoxigenin or biotin. The authors tested the ability of the labeled growth factors to stimulate proliferation of the human megakaryoblastic leukemia cell line, M-07e; the human erythroleukemia cell line, TF1; and the human plasmacytoma cell line T1165. Cell proliferation was assayed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2531)

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J. Biol. Chem. 272, 14532-14541. Cytotoxicity and apoptosis produced by arachidonic acid in Hep G2 cells overexpressing human cytochrome P4502E1. 1997

Chen, Q. , Galleano, M. , Cederbaum, A. I.

Notes: Both sense and antisense bcl-2 and cytochrome P4502E1 cDNAs were stably expressed in a HepG2 derived cell line using the pCI-Neo Mammalian Expression Vector. Cytotoxicity of arachidonic acid to transfected and control cells was assessed using the CellTiter 96® Non-Radioactive Cell Proliferation Assay. (1328)

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Blood 90, 1751-1767. Development of a candidate HLA A*0201 restricted peptide-based vaccine against human cytomegalovirus infection. 1997

Diamond, D.J. , York, J. , Sun, J.Y. , Wright, C.L. , Forman, S. J.

Notes: Fragments of the pp65 protein cDNA were subcloned into a modified pCI-neo Mammalian Expression Vector containing an enterokinase cleavage site and 6XHis tag. These fusion vectors were co-transfected into COS cells with an HLA A*0201 gene-expressing pCI-Neo Vector construct. The transfected cells were assayed for TNFα production. TNFalpha was quantified by its cytotoxicity to WEHI-164 cells using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS). (1231)

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J. Clin. Invest. 100(1), 149-157. Ectopic expression of decorin protein core causes a generalized growth suppression in neoplastic cells of various histogenetic origin and requires endogenous p21, an inhibitor of cyclin-dependent kinases. 1997

Santra, M., Mann, D.M., Mercer, E.W., Skorski, T., Calabretta, B., and Iozzo, R.V.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to measure suppression of the number of proliferating cells expressing or growing in the presence of decorin. Various cell types were used including Saos-2 osteosarcoma, HL-60 promyelocytic, HT-1080 fibrosarcoma, HeLa cervical carcinoma, M2 mouse melanoma, and HCT116 colon carcinoma cells. (1703)

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J. Clin. Invest. 100, 149-157. Ectopic Expression of Decorin Protein Core Causes a Generalized Growth Suppression in Neoplastic Cells of Various Histogenetic Origin andRequires Endogenous p21, an Inhibitor of Cyclin-dependent Kinases 1997

Santra, M., Mann, D.M., Mercer, E.W., Skorski, T., Calabretta, B., Iozzo, R.V.

Notes: CellTiter96® AQueous Non-Radioactive Cell Proliferation Assay was used to measure proliferative responses in various cancer cell lines: HeLa (cervical carcinoma); Saos-2 (osteosarcoma); ht-1080 (Fibrosarcoma); M2 (melanoma); and HCT116 (colon carcinoma). (0439)

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J. Biol. Chem. 272, 16540-16547. Epidermal growth factor and okadaic acid stimulate Sp1 proteolysis. 1997

Mortensen, E.R., Marks, P.A., Shiotani, A., Merchant, J.L.

Notes: Used the Luciferase Assay System and the CellTiter 96® AQueous Cell Proliferation Assay (MTS/PMS) in their studies on GH4 rat pituitary adenoma cells. Reporter constructs were prepared in the pGL2 Basic Vector. (0667)

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Blood 89(7), 2443-2452. Human cytomegalovirus-associated immunosuppression is mediated through Interferon-alpha. 1997

Noraz, N., Lathey, J.L. and Spector, S.A.

Notes: The CellTiter 96® AQueous (MTS/PMS) Non-Radioactive Cell Proliferation Assay was used to measure HCMV-associated suppression of oxidative activity of peripheral blood mononuclear cells (PBMC) from healthy human donors. (2078)

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Am. J. Physiol. 273, C1987-C1994. Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-beta 1. 1997

Chang, N. S.

Notes: In this paper, the CellTiter® 96 AQueous Non-Radioactive Cell Proliferation Assay was used to study TNF-induced toxicity and rescue by TGF-Beta1 of  LNCap, L929, and Mv 1 Lu cells. (1361)

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J. Mol. Neurosci. 9, 211-222. Identification of VIP/PACAP receptors on rat astrocytes using antisense oligodeoxynucleotides. 1997

Ashur-Fabian, O., Giladi, E., Brenneman, D.E., and Gozes, I.

Notes: The MTS-based CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to measure viability of rat glial cells treated with antisense oligodeoxynucleotide. (1539)

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Nature 388, 190-195. Inhibition of death receptor signals by cellular FLIP 1997

Irmler, M., Thome, M., Hahne, M., Schneider, P., Hofmann, K., Steiner, V., Bodmer, J-L., Schröter, Burns, K., Mattman, C., Rimoldi, D., French, L.E., Tschopp, J.

Notes: This paper reports the characterization of the apoptosis inhibitor protein, FLIP. FLIP-transfected Jurkat and Raji clones were assayed for sensitivity to FasL, sTRAIL, and staurosporine using the CellTiter 96® AQueous Non-Radioactive Assay. Staurosporine-induced apoptosis was not inhibited by FLIPs. (2517)

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Am. J. Respir. Cell Mol. Biol. 17(5), 541-551. Interleukin-4 alters epithelial cell differentiation and surfactant homeostasis in the postnatal mouse lung. 1997

Jain-Vora, S., Wert, S.E., Temann, U.A., Rankin, J.A., Whitsett, J.A.

Notes: The MTS-based CellTiter 96® AQueous System was used to assess the proliferative response of T-cells (20,000 per well) to mitomycin C-treated splenocyte antigen-presenting cells (10,000 per well) and Concanavalin A. T-cells isolated from the lung and spleen of normal mice were compared to transgenic mice expressing IL-4 constitutively in lung tissue. The lung T-cells from the transgenic mice proliferated in response to the antigen-presenting cells. (2989)

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J. Cell Biol. 139, 1017-1023. Lack of correlation between activation of Jun-NH2-terminal kinase and induction of apoptosis after detachment of epithelial cells. 1997

Khwaja, A., Downward, J.

Notes: The CellTiter® 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to measure cell MDCK cell survival during an anoikis assay. (0938)

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