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Blood 93, 537-553. Synergistic activation of mitogen-activated protein kinase by cyclic AMP and myeloid growth factors opposes cyclic AMP's growth-inhibitory effects. 1999

Lee, A. W.

Notes: The 32D cell line was transfected with the colony stimulated factor-1 receptor. The cells were cultured with either IL-3 or CSF-1 with various amounts of dibutryl-cAMP or forskolin. The CSF-1 treated cells proliferated more closely to untreated cells than the IL-3-treated cells. Proliferation was measured with the CellTiter 96® AQueous Assay (MTS/PMS). (0807)

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J. Biol. Chem. 274, 24987-24994. The carboxyl terminal extension of the Drosophila insulin receptor homologue binds IRS-1 and influences cell survival 1999

Marin-Hincapie, M., Garofalo, R.S.

Notes: MDCK and HEK 293 cells were transfected with DNA encoding the beta subunit of the Drosophila homolog of the insulin receptor or with DNA encoding the subunit with the C-terminal extension deleted. The authors used the CellTiter® 96 AQueous Non-Radioactive Cell Proliferation Assay to assess the effect of expression of these two constructs on cell growth. (2501)

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J. Immunol. 163, 5954-5963. The CXC chemokine stromal cell-derived factor activates a Gi-coupled phosphoinositide 3-kinase in T lymphocytes 1999

Sotsios, Y., Whittaker, G.C., Westwick, J., Ward, S.G.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to determine the amount of Jurkat cells that had migrated through a chemotaxis chamber. (0357)

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Proc. Natl. Acad. Sci. USA 96(6), 3087-91. Transforming growth factor beta stimulation of colorectal cancer cell lines: type II receptor bypass and changes in adhesion molecule expression. 1999

Ilyas, M., Efstathiou, J.A., Straub, J., Kim, H.C., and Bodmer, W.F.

Notes: The presence of type II TGFβ receptor mutations in 12 colorectal cancer cell lines (LS174T, HCA7, LOVO, SW48, DLD1, HCT116, COLO201, COLO205, COLO320, HCA46, HT29, and CACO2) was examined. Six cell lines were found to have homozygous mutations. Growth inhibition upon TGFβ1 stimulation and changes in adhesion molecule expression of these cell lines was also studied. Cells were stimulated with Promega's human TGFβ1 at a concentration of 2.5 ng/ml and cell proliferation was measured using the CellTiter 96® AQueous Nonradioactive Cell Proliferation Assay. Two cell lines were shown to be inhibited by TGFβ1 despite homozygous mutations in the type II TGFβ receptor gene. As a control, TGFβ1 was bioneutralized with the Anti-TGFβ1 pAb at a 2:1 molar ratio of antibody to TGFβ1. (2457)

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J. Biol. Chem. 274, 34811-34818. Tumor cell viability in clear cell sarcoma requires DNA binding activity of EWS/ATF1 fusion protein 1999

Bosilevac, J.M., Olsen, R.J., Bridge, J.A., Hinrichs, S.H.

Notes: Contribution of the EWS/ATF1 chimeric protein to tumor phenotype was investigated by expressing an inhibitory anti-AFT1 single chain antibody in cells derived from clear cell sarcoma. Cell viability was monitored using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2543)

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Biochemistry 37, 6533-6540. A novel tetraester construct that reduces cationic lipid-associated cytotoxicity, implication for the onset of cytotoxicity 1998

Aberle, A.M., Tablin, F., Zhu, J., Walker, N.J., Gruenert, D.C. and Nantz, M.H.

Notes: Various lipid formulations were used to transfect NIH 3T3 cells with the pGL3 Control Vector to monitor the transfection. The cytotoxic effect of these lipid combinations were measured with the CellTiter 96® AQueous Proliferation Assay (MTS/PMS). (2054)

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Blood 91, 1625-1632. A proteasome inhibitor, an antioxidant, or a salicylate, but not a glucocorticoid, blocks constitutive and cytokine-inducible expression of P-selectin in human endothelial cells 1998

Xia, L. , Pan, J. , Yao, L. , McEver, R. P.

Notes: The CellTiter® 96 AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to monitor the viability of human umbilical vein epithelial cells and bovine aortic endothelial cells following treatment with various pharmacological reagents. (0163)

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Mol. Pharmacol. 54, 825-833. Aryl hydrocarbon receptor regulation of cytochrome P450 1B1 in rat mammary fibroblasts: Evidence for transcriptional repression by glucocorticoids 1998

Brake, P.B., Zhang, L., Jefcoate, C.R.

Notes: The authors investigated the effect of the glucocorticoid, dexamethasone, on the proliferation of primary cultures of rat mammary fibroblasts (RMF) using the  CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2488)

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J. Biol. Chem. 273, 2322-2328. Cell to cell contact is not required for bystander cell killing by Escherichia coli purine nucleoside phosphorylase. 1998

Hughes, B.W., King, S.A., Allan, P.W., Parker, W.B., Sorscher, E.J.

Notes: The authors used CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) with human and mouse glioma cells (0987)

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J. Biol. Chem. 273, 18514-18521. Chemotactic properties of angiopoietin-1 and -2, ligands for the endothelial-specific receptor tyrosine kinase Tie2 1998

Witzenbichler, B., Maisonpierre, P.C., Jones, P., Yancopoulos, G.D., Isner, J.M.

Notes: HUVECs were stimulated with either recombinant angiopoetin-1 or -2 or VEGF, and cell proliferation was assayed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2511)

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J. Exp. Med. 187, 1205-1213. Conversion of membrane-bound Fas (CD95) ligand to its soluble form is associated with downregulation of its proapoptotic activity and loss of liver toxicity. 1998

Schneider, P., Holler,N., Bodmer,J.-L., Hahne, M., Frei, K., Fontana, A., Tschopp, J.

Notes: The CellTiter 96® AQueous Cell Proliferation Assay was used to determine the viability of Jurkat, BJAB, HT-29, A20 and WEHI164  cells in response to various soluble factors like soluble Fas Ligand and soluble TNF-related apoptosis-inducing ligand. Antibody-induced aggregation of the ligand increased the cytotoxicity. Good detail is provided for the assay. (0413)

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Antimicrob. Agents Chemother. 42, 843-848. Cyclosporin analogs inhibit in vitro growth of Cryptosporidium parvum 1998

Perkins, M.E., Wu, T.W., LeBlanco, S.M.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to demonstrate that the cyclosporin treatment had no cytotoxic effect on the Cryptosporidium parvum host cell, Caco-2 cells. (0560)

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J. Biol. Chem. 273, 19411-19418. Cytoplasmic domains of the human granulocyte-macrophage colony-stimulating factor (GM-CSF) receptor β chain (hβc) responsible for human GM-CSF-induced myeloid cell differentiation 1998

Matsuguchi, T., Lilly, M.D., Kraft, A.S.

Notes: WT19 (myeloblast-like mouse cells) expressing the α-subunit along with wildtype or mutant β-subunit of the GM-CSF receptor were incubated with various concentrations of human GM-CSF and cell proliferation was assayed using the CellTiter 96® AQueous Cell Proliferation Assay. (2554)

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J. Clin. Invest. 101, 406-412. Decorin suppresses tumor cell growth by activating the epidermal growth factor receptor 1998

Mostatello, D.K., Santra, M., Mann, D.M., McQuillan, D.J., Wong, A.J., Iozzo, R.V.

Notes: The authors investigated the mechanism through which the extracellular matrix proteoglycan, decorin, inhibits cell cycle progression. The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to determine growth curves for A431 squamous carcinoma cells transfected with a variety of decorin clones. The assay was also used to determine the effects of exogenous human recombinant decorin, decorin protein or biglycan on wildtype A431 cells. The authors also measured the ability of tyrphostin AG1468 to prevent the growth-inhibitory effects of decorin in A431 using the same assay. (2475)

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J. Med. Chem. 41, 981-987. Design, synthesis and evaluation of the multidrug resistance-reversing activity of D-glucose mimetics of hapalosin. 1998

Dinh, T.Q., Smith, C. D., Du, X. and Armstrong, R. W.

Notes: The MTS-based CellTiter 96® AQueous One Solution Cell Proliferation Assay was used to follow the survival of HL-60/ADR cells to the cytotoxic agent, vincristine. Various compounds were tested to inhibit the multidrug resistance protein-mediated survival of the cells. (1501)

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J. Biol. Chem. 273, 19685-19690. Differential effects of peptide histidine isoleucine (PHI) and related peptides on stimulation and suppression of neuroblastoma cell proliferation: A novel VIP-independent action of PHI via MAP kinase. 1998

Lelièvre, V., Pineau, N., Du, J., Wen, C.-H., Nguyen, T., Janet, T., Muller, J.-M., Waschek, J.A.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to measure the proliferation of Neuro2a cells in response to various peptides. The cells were plated at 8000 cell/well 24hr prior to addition of the peptides. Forty-eight hours later, the media was removed new media added and the proliferation assay performed. (0826)

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Antimicrob. Agents Chemother. 42, 1959-1965. Efficacy of nitaoxanide against Cryptosporidium parvum in cell culture and animal models 1998

Theodos, C.M., Griffiths, J.K., D'onfro, J., Fairfield, A., Tzipori, S.

Notes: The cytotoxicities of nitaoxanide and paromomycin to MDBK cells were assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2529)

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J. Immunol. 161, 3936-3942. FLIP prevents apoptosis induced by death receptors but not by perforin/granzyme B, chemotherapeutic drugs, and gamma irradiation 1998

Kataoka, T., Schroter, M., Hahne, M., Schneider, P., Irmler, M., Thome, M., Froelick, C.J., Tschopp, J.

Notes: To assay for activation-induced cell death, Jurkat cells were plated in wells coated with anti-human CD3 antibodies. Cell viability was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2512)

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Am. J. Physiol. 44, c1087-c1094. Heme oxygenase-1 induction in skeletal muscle cell; hemin and soidum nitroprusside are regulators in vitro 1998

Vesely, M.J., Exon, D.J., Clark, J.E., Foresti, R., Green, C.J., Motterlini, R.

Notes: L6.G8 cells were seeded in 96-well plates and grown to confluence and then incubated with hemin or sodium nitroprusside for 6 hours. Cell viability was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2520)

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Gastroenterology 114, 930-939. Inhibition of epidermal growth factor receptor kinase induces protease-dependent apoptosis in human colon cancer cells. 1998

Karnes, Jr., W.E., Weller, SS.G., Adjei, P.N., Kottke, T.J., Glenn, K.S., Gores, G.J., Kaufmann, S.H.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay was used to assess changes in viable cell mass of SNU-C1, SNU-C4 and SNU-C5 colon cancer cell lines following treatment with EGFR tyrosine kinase inhibitors. (0961)

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Blood 91, 4265-4272. Interleukin-15 (IL-15) can replace the IL-2 signal in IL-2-dependent adult T-cell leukemia (ATL) cell lines: Expression o f IL-15 receptor α on ATL cells 1998

Yamada, Y., Sugawara, K., Hata, T., Tsuruta, K., Moriuchi, R., Maeda, T., Atogami, S., Murata, K., Fujimoto, K., Kohno, T., Tsukasaki, K., Tomonaga, M., Hirakata, Y., Kamihira, S.

Notes: Four adult T-cell leukemia cell lines were cultured with rIL-2, and cell proliferation was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2519)

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Hepatology 27, 1233-1240. Loss of butyrate-induced apoptosis in human hepatoma cell lines HCC-M and HCC-T having substantial Bcl-2 expression 1998

Saito, H., Ebinuma, H., Takahashi, M., Kaneko, F., Wakabayashi, K., Nakamura, M., Ishii, H.

Notes: Cell growth of human hepatoma cell lines, HCC-M and HCC-T was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2516)

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J. Biol. Chem. 273, 3654-3660. Midkine induces tumor cell proliferation and binds to a high affinity signaling receptor associated with JAK tyrosine kinases. 1998

Ratovitski, E.A., Kotzbauer, P.T , Milbrandt, J., Lowenstein, C.J. , Burrow, C.R.

Notes: The CellTiter® 96 AQueous Cell Proliferation Assay (MTS/PMS) was used to assess the proliferative activity of Pleiotrophin and Midkine on NIH3T3, normal rat kidney cells, and the G401 kidney rhabdoid tumor cells. The assay was also used to assay the mitogenic activity of Pleiotrophin and midkine on G401 cells from 4-120 hours. (0501)

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Stem Cells 16, 54-60. Neutralization of biological activity and inhibition of receptor binding by antibodies against human thrombopoietin 1998

Tahara, T., Kuwaki, T., Matsumoto, A., Morita, H., Watari, H., Inagaki, Y., Ohashi, H., Ogami, K., Miyazaki, H., Kato, T.

Notes: Thrombopoietin (TPO)  is a cytokine that regulates megakaryocytopoiesis and thrombopoiesis. These authors raised antibodies to peptides derived from TPO and rhTPO in order to characterize immunologically distinct regions of TPO. They assessed the ability of the antibodies to interfere with TPO-stimulated cell proliferation of FDCP-2 cells that constitutively express human Mpl, the receptor for TPO, using the CellTiter 96® AQueous Non-Radioactive Cell Proliferatioin Assay. They show dose-dependent inhibition of rhTPO-induced growth of these cells by specific antibodies. (2486)

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Oncogene 16, 2087-2094. Overexpression of both p185c-erbB2 and p170mdr-1 renders breast cancer cells highly resistant to taxol. 1998

Yu, D., Liu, B., Jing, T., Sun, D., Price, J.E., Singletary, S.E., Ibrahim, H., Hortobagyi, G.N., Hung, M.-C.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to monitor the viablity of various breast cancer cell line following treatment with taxol. (0119)

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