Ilyas, M., Efstathiou, J.A., Straub, J., Kim, H.C., and Bodmer, W.F.
Notes: The presence of type II TGFβ receptor mutations in 12 colorectal cancer cell lines (LS174T, HCA7, LOVO, SW48, DLD1, HCT116, COLO201, COLO205, COLO320, HCA46, HT29, and CACO2) was examined. Six cell lines were found to have homozygous mutations. Growth inhibition upon TGFβ1 stimulation and changes in adhesion molecule expression of these cell lines was also studied. Cells were stimulated with Promega's human TGFβ1 at a concentration of 2.5 ng/ml and cell proliferation was measured using the CellTiter 96® AQueous Nonradioactive Cell Proliferation Assay. Two cell lines were shown to be inhibited by TGFβ1 despite homozygous mutations in the type II TGFβ receptor gene. As a control, TGFβ1 was bioneutralized with the Anti-TGFβ1 pAb at a 2:1 molar ratio of antibody to TGFβ1. (2457)