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J. Biol. Chem. 275, 3328-3334. Truncation of the β-catenin binding domain of E-caherin precedes epithelial apoptosis during prostate and mammary involution 2000

Vallorosi, C.J., Day, K.C., Zhao, X., Rashid, M.G., Rubin, M.A., Johnson, K.R., Wheelock, M.J., Day, M.L.

Notes: The authors used the CellTiter 96® AQueous Assay to measure the cell viability of cultured LNCaP prostate epitheilial cells or SUM185 mammary epithelial cells. (2526)

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J. Biol. Chem. 275, 7138-7143. Uncoupling ceramide glycosylation by transfection of glucosylceramide synthase antisense reverses adriamycin resistance. 2000

Liu, Y.Y., Han, T.Y., Giuliano, A.E., Hansen, N., Cabot, M.C.

Notes: The CellTiter 96® AQueous Assay (MTS/PMS) was used to follow the cytotoxic effect of adriamycin and ceramide on MCF-7 cells transfected with either the sense or antisense adriamycin receptor. (0782)

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J. Biol. Chem. 274, 8669-8677. A cell type-specific constitutive point mutant of the common β-subunit of the human granulocyte-macrophage colony-stimulating factor (GM-CSF), Interleukin (IL)-3, and IL-5 receptors requires the GM-CSF receptor α-subunit for activation. 1999

Jenkins, B.J., Le, F., Gonda, T.J.

Notes: The CellTiter 96® Non-Radioactive Cell Proliferation Assay was used to assay the proliferation of BAF-B03, a normally IL-3-dependent cell line, infected with a retrovirus encoding the murine GM-CSF receptor α subunit (GMRa) only or GMRa and the mutant β-chain signal transduction fragment containing the mutation I374N. The infected cell line with the wildtype receptor could now proliferate in response to GM-CSF or Il-3 but did not grow in the absence of either factor. The cell line infected with the I374N mutant and the GM-CSF receptor α subunit could proliferate without either factor. These two mutants also gave self-proliferative properties to CTLL-2 cells. The mutant β chain protein was generated with the Altered Sites® II in vitro Mutagenesis System. (0973)

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J. Immunol. 163, 250-258. A single-chain IL-12 IgG3 antibody fusion protein retains antibody specificity and IL-12 bioactivity and demonstrates antitumor activity 1999

Peng, L.S., Penichet, M.L., Morrison, S.L.

Notes: Cell proliferation of mouse peripheral mononuclear cells was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2539)

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Proc. Natl. Acad. Sci. USA 96, 2811-2816. Angiostatin binds ATP synthase on the surface of human endothelial cells 1999

Moser, T.L., Stack, M.S., Asplin, I., Enghild, J.J., Horjup, P., Everitt, L., Hubchak, S., Schnaper, H.W., Pizzo, S.V.

Notes: The proliferative effect of angiostatin on Human Umbilical Vein Endothelial Cells (HUVEC) was investigated using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. Cells were incubated with angiostatin in the presence or absence of antibodies raised against the α-subunit of ATP synthase (a binding site for angiostatin). The antibodies blocked the anti-proliferative effect of angiostatin on HUVEC. (2508)

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Biochem. J. 341, 765-769. Autocrine regulation of growth stimulation in human epithelial ovarian carcinoma by serine-proteinase-catalysed release of the urinary-type-plasminogen-activator N-terminal fragment. 1999

Fishman, D. A. , Kearns, A. , Larsh, S. , Enghild, J. J. , Stack, M. S.

Notes: The CellTiter 96® AQueous Assay (MTS/PMS) was used to measure the proliferation of the DOV13 human ovarian epithelial cell carcinoma cell in the presence of n-terminal fragment of urinary-type plasminogen activator and antibodies to the fragment. (1158)

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Mol. Cell. Biol. 19, 1171-1181. Biological effects of c-Mer receptor tyrosine kinase in hematopoietic cells depend on the Grb2 binding site in the receptor and activation of NF-kappaB. 1999

Georgescu, M. M. , Kirsch, K. H. , Shishido, T. , Zong, C. , Hanafusa, H.

Notes: The IL3-dependent Ba/F3 cells were transfected with the c-Mer tyrosine kinase receptor made constitutively active by linking the CD8 extracellular domain and the cMer intracellular domain. Essentially the cells became IL3-independent. Cell proliferation was measured with the CellTiter 96® AQueous Assay (MTS/PMS). (1149)

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J. Biol. Chem. 274, 16582-16589. CCAAT/enhancer-binding protein delta regulates mammary epithelial cell G0 growth arrest and apoptosis 1999

O'Rourke, J.P., Newbound, G.C., Hutt, J.A., DeWille, J.

Notes: The authors investigated the effect of altered C/EBPδ content on G0 growth arrest and apoptosis in mammary epithelial cell lines. The pGEM-4Z cloning vector was used in a cloning strategy to create a RNA antisense plasmid. For overexpression, HC11 cells were transfected with an overexpression plasmid using Promega's Transfectam® Reagent. Cell proliferation of HC11, HC11/antisense and HC11/overexpression cell lines was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (0565)

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Biochem. J. 344, 39-46. Cellular activation by Ca2+ release from stores in the endoplasmic reticulum but not by increased free Ca2+ in the cytosol. 1999

Strayer, D. S., Hoek, J. B., Thomas, A. P., White, M. K.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to assess the viability of the primary rat type II aveolar pneumocytes to calcium chelator BAPTA. No significant decrease in viability was observed. (0345)

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J. Neurosci. 19, 5159-5172. Cellular defects and altered gene expression in PC12 cells stably expressing mutant Huntingtin 1999

Li, S-H., Cheng, A.L., Li, H., Li, X-J.

Notes: PC12 were cells stably transfected with the Huntingtin exon-1 protein with expanded polyglutamine (150Q), and cell viability was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay.  Apoptosis was also measured using TUNEL staining with the DeadEnd™ Colorimetric TUNEL System. (2541)

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FASEB J. 13, 1950-1960. Fas (CD95, APO-1) antigen expression and function in murine liver endothelial cells: implications for the regulation of apoptosis in liver endothelial cells. 1999

Cardier, J. E. , Schulte, T. , Kammer, H. , Kwak, J. , Cardier, M.

Notes: The CellTiter 96® AQueous Assay (MTS/PMS) was used to examine the toxicity of anti-Fas on the LEC-1, mouse liver endothelial cell line. (1345)

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Biochem. J. 338, 637-642. Heparin and heparan sulphate protect basic fibroblast growth factor from non-enzymic glycosylation. 1999

Nissen, N. N. , Shankar, R. , Gamelli, R. L. , Singh, A. , DiPietro, L. A.

Notes: Basic FGF was glycosylated by non-enzymatic means and tested for HUVEC cell proliferation. The proliferation was measured with the procedure of the CellTiter 96® AQueous Non-Radioactive Assay using reagents prepared for MTS Powder (Promega) and phenazine methosulphate (PMS; Sigma). (0621)

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J. Biol. Chem. 274, 32382-32386. Hyperglycemia enhances angiotensin II-induced janus-activated kinase/STAT signaling in vascular smooth muscle cells. 1999

Amiri, F., Venema, V.J., Wang, X., Ju, H., Venema, R.C. and Marrero, M.B.

Notes: The CellTiter 96® AQueous Cell Proliferation Assay was used to monitor the proliferation of rat vascular smooth muscle cells (VSMCs) in response to angiotensin II and high glucose media, angiotensin II and normal glucose media, and high glucose media alone. (1510)

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J. Biol. Chem. 274(43), 30459-3-467. Identification of glucagon-like peptide-2(GLP-2)-activated signaling pathways in baby hamster kidney fibroblasts expressing the rat GLP-2 receptor 1999

Yusta, B., Somwar, R., Wang, F., Munroe, D., Grinstein, S., Klip, A., Drucker, D.J.

Notes: The authors investigated the mechanism of stimulation of growth in intestinal epitheilium by glucagon-like peptide 1 (GLP-2). Baby hamster kidney fibroblasts (BHK) were transfected with the rat GLP-2 receptor and assayed for downstream effects upon treatment with GLP-2.  Cell proliferation assays to determine the effects of GLP-2 on cell viability were carried out using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2480)

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J. Immunol. 163, 6132-6138. Inhibition of oligodendrocyte apoptosis by sublytic C5b-9 is associated with enhanced synthesis of Bcl-2 and mediated by inhibition of caspase-3 activation 1999

Soane, L., Rus, H., Niculescu, F., Shin, M.L.

Notes: Viability of O-2A cells during differentiation and the effect of the C5b-9 membrane attack complex of complement was determined using the CellTiter 96® AQueous Cell Proliferation Assay. (2538)

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Proc. Natl. Acad. Sci. USA 96, 2678-2681. Lysozyme and RNases as anti-HIV components in β-core preparations of human chorionic gonadotropin 1999

Lee-Huang, S., Huang, P.L., Sun, Y., Huang, P.L., Kung, H-F., Blithe, D. L., Chen, H-C.

Notes: The authors used the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay to determine whether cell viability of  chronically HIV-infected ACH-2 lymphocytes and U1 monocytes was affected by anti-viral lysozyme and anti-viral RNase obtained from human chorionic gonadotropin preparations. (2477)

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J. Biol. Chem. 274, 29944-29950. Mutants of interleukin 13 with altered reactivity toward interleukin 13 receptors 1999

Thompson, J.P., Debinski, W.

Notes: TF-1 pre-leukemic human B cells were grown in the presence of wildtype or mutant IL-13. After 72 hours in the growth media, the cell proliferation was determined with the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. Cell proliferation was reported as percent increase over control, no interleukin values. (0262)

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J. Immunol. 162, 5337-5344. NF-κB-inducing kinase is a common mediatorof IL-17, TNF-α, and IL-1-β- induced chemokine promoter activation in intestinal epithelial cells 1999

Awane, M., Andres, P.G., Li, D.J., Reinecker, H-C.

Notes: IEC-6 cells were seeded into 96-well plates and cultured in the presence of various concentrations of human IL-17. Cell proliferation was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. The pGL3 vector was used to clone a PCR-amplified piece of the 5' flanking region of the CINC gene, and the Luciferase Assay System was used to assess promoter activation. All transfections of IEC-6 cells used the pSV-β-galactosidase vector as a control for transfection efficiency. Total RNA for Northern blotting was isolated using the Poly ATtract System. (2510)

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EMBO J. 18, 1824-1831. Oncogenic Ras inhibits Fas ligand-mediated apoptosis by downregulating the expression of Fas. 1999

Peli, J., Schroter, M., Rudaz, C., Hahne, M., Meyer, C., Reichmann, E., Tschopp, J.

Notes: The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay (MTS/PMS) was used to analyze the effect of expression of H-ras in Ep mouse mammary epithelial cells and NIH-3T3 cells. The H-ras-expressing cells demonstrated marked resistance to the Fas ligand when compared to their untransfected counterparts. (0557)

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EMBO J. 18, 5601-5608. p38 MAP kinase is required for STAT1 serine phosphorylation and transcriptional activation induced by interferons. 1999

Goh, K.C., Haque, S.J., Williams, B.R.

Notes: The CellTiter 96® AQueous Assay (MTS/PMS) was used to assess the viability of the cells expressing mutant or wildtype p38 kinases on IFN-γ protection of viral killing. (1108)

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Biochem. J. 339, 729-736. Peroxynitrite induces haem oxygenase-1 in vascular endothelial cells: a link to apoptosis. 1999

Foresti, R. , Sarathchandra, P. , Clark, J. E. , Green, C. J. , Motterlini, R.

Notes: The CellTiter 96® AQueous Assay (MTS/PMS) was used to measure the cytotoxic effects of various compounds including peroxynitrite, n-acetylcysteine, uric acid and protoporphyrin IX to bovine aortic endothelial cells. (1164)

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Proc. Natl. Acad. Sci. USA 96, 3496-3501. Phthalascidin, a synthetic antitumor agent with potency and mode of action comparable to ecteinascidin 743 1999

Martinez, E.J., Owa, T., Screiber, S. L., Corey, E.J.

Notes: The authors used Promega's CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay to investigate the ability of phthalascidin, ecteinascidin 743 and a key intermediate to inhibit cell proliferation in a variety of human cancer cell lines. Cell lines used in the study were purchased from ATCC and included: A-549 and NCI-H22 lung carinoma lines; HCT116 and COLO205 colon carinoma lines; MCF-7 and T-47D breast carcinoma lines; A375 malignant melanoma; and PC-3 prostate carcinoma. (2474)

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J. Clin. Invest. 104, 1469-1480. Regulation of smooth muschle cell migration and integrin expression by the Gax transcription factor 1999

Witzenbichler, B., Kureishi, Y., Luo, Z., Le Roux, A., Branellec, D., Walsh, K.

Notes: Cell viability of rat vascular smooth muscle cells infected with recombinant Adenovirus containing the rat gax gene, human p16 or human p21, or the β-galactosidase gene was assessed using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2548)

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Proc. Natl. Acad. Sci. USA 96, 4325-4329. Selective killing of transformed cells by cyclin/cyclin-dependent kinase 2 antagonists 1999

Cehn, Y-N.P., Sharma, S.K., Ramsey, T.M., Jiang, L., Martin, M.S., Baker, K., Adams, P.D., Bair, K.W., Kaelin, W.G.

Notes: Cells were incubated with peptides containing a motif that serves as a docking site for cyclin/cyclin-dependent kinase 2 complexes. The authors measured the viability of cells incubated with these peptides using the CellTiter® 96 AQueous Non-Radioactive Cytotoxicity Assay. (2500)

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Proc. Natl. Acad. Sci. USA 96, 3700-3705. Suppression of breast cancer growth and metastasis by a serpin myoepithelium-derived serine proteinase inhibitor expressed in the mammary myoepithelial cells. 1999

Xiao, G., Liu, Y.E., Gentz, R., Sang, Q.A., Ni, J., Goldberg, I.D., Shi, Y.E.

Notes: Exponentially growing cultures of different MDA-MB-435 clones were detached with trypsin, and the trypsin was neutralized with DMEM/10% serum. Cells were counted, diluted, and seeded in triplicate. Cell growth was measured using the CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay. (2528)

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